中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2014年
2期
85-88
,共4页
陈燕%时红%顾琳%彭晓谋
陳燕%時紅%顧琳%彭曉謀
진연%시홍%고림%팽효모
前蛋白转化酶类%RNA,信使%肿瘤坏死因子α%肝炎病毒,乙型%病毒复制
前蛋白轉化酶類%RNA,信使%腫瘤壞死因子α%肝炎病毒,乙型%病毒複製
전단백전화매류%RNA,신사%종류배사인자α%간염병독,을형%병독복제
Proprotein convertases%RNA,messenger%Tumor necrosis factor-alpha%Hepatitis B virus%Virus replication
目的 探讨TNF-α对原蛋白转化酶(PC)表达的影响,以及与其抑制HBV复制作用的关系.方法 常规培养的HepG2.2.15细胞加20 μg/L重组TNF-α或(和)20 μmol/L PC抑制剂(DEC),作用18h,收集细胞,提取细胞总RNA和衣壳相关HBV DNA,采用荧光定量PCR技术检测PC mRNA和HBV DNA.计量资料比较采用t检验.结果 以空白对照组的表达水平为基数(1),20 μg/L重组TNF-α作用18 h后,HepG2.2.15细胞内7种PC mRNA均显著上调(PC1/3、PC2、furin、PC4、PC5/6、PACE4和PC7/8 mRNA的表达水平分别为3.3±0.7、79.3±3.3、77.5±1.3、19.2±3.1、1.3±0.1、1.4±0.2、274.8±7.1,均P＜0.05).与空白对照组相比,20 μg/L重组TNF-α作用18h后,衣壳相关HBV DNA水平明显降低(0.21∶1,t=8.79,P=0.002),20 μmol/L DEC显著升高衣壳相关HBV DNA水平(3.84∶1,t=7.67,P=0.004),而DEC和TNF-α联合处理组的衣壳相关HBV DNA水平显著高于TNF-α处理组(0.31∶021,t=10.49,P=0.007).结论 TNF-α上调PC mRNA表达是其抑制HBV复制的重要中间环节.
目的 探討TNF-α對原蛋白轉化酶(PC)錶達的影響,以及與其抑製HBV複製作用的關繫.方法 常規培養的HepG2.2.15細胞加20 μg/L重組TNF-α或(和)20 μmol/L PC抑製劑(DEC),作用18h,收集細胞,提取細胞總RNA和衣殼相關HBV DNA,採用熒光定量PCR技術檢測PC mRNA和HBV DNA.計量資料比較採用t檢驗.結果 以空白對照組的錶達水平為基數(1),20 μg/L重組TNF-α作用18 h後,HepG2.2.15細胞內7種PC mRNA均顯著上調(PC1/3、PC2、furin、PC4、PC5/6、PACE4和PC7/8 mRNA的錶達水平分彆為3.3±0.7、79.3±3.3、77.5±1.3、19.2±3.1、1.3±0.1、1.4±0.2、274.8±7.1,均P＜0.05).與空白對照組相比,20 μg/L重組TNF-α作用18h後,衣殼相關HBV DNA水平明顯降低(0.21∶1,t=8.79,P=0.002),20 μmol/L DEC顯著升高衣殼相關HBV DNA水平(3.84∶1,t=7.67,P=0.004),而DEC和TNF-α聯閤處理組的衣殼相關HBV DNA水平顯著高于TNF-α處理組(0.31∶021,t=10.49,P=0.007).結論 TNF-α上調PC mRNA錶達是其抑製HBV複製的重要中間環節.
목적 탐토TNF-α대원단백전화매(PC)표체적영향,이급여기억제HBV복제작용적관계.방법 상규배양적HepG2.2.15세포가20 μg/L중조TNF-α혹(화)20 μmol/L PC억제제(DEC),작용18h,수집세포,제취세포총RNA화의각상관HBV DNA,채용형광정량PCR기술검측PC mRNA화HBV DNA.계량자료비교채용t검험.결과 이공백대조조적표체수평위기수(1),20 μg/L중조TNF-α작용18 h후,HepG2.2.15세포내7충PC mRNA균현저상조(PC1/3、PC2、furin、PC4、PC5/6、PACE4화PC7/8 mRNA적표체수평분별위3.3±0.7、79.3±3.3、77.5±1.3、19.2±3.1、1.3±0.1、1.4±0.2、274.8±7.1,균P＜0.05).여공백대조조상비,20 μg/L중조TNF-α작용18h후,의각상관HBV DNA수평명현강저(0.21∶1,t=8.79,P=0.002),20 μmol/L DEC현저승고의각상관HBV DNA수평(3.84∶1,t=7.67,P=0.004),이DEC화TNF-α연합처리조적의각상관HBV DNA수평현저고우TNF-α처리조(0.31∶021,t=10.49,P=0.007).결론 TNF-α상조PC mRNA표체시기억제HBV복제적중요중간배절.
Objective To investigate the effects of tumor-necrosis factor α (TNF-α) on the expressions of proprotein convertases (PC) and its relationship with the inhibition of hepatitis B virus (HBV) replication.Methods HepG2.2.15 cells cultured routinely were exposed to 20 μg/L recombinant TNF-α and/or 20 μmol/L PC inhibitor (DEC) for 18 h.Then Followed cells werecollected and cell total RNA and HBV DNA were extracted.PC mRNA and core-associated HBV DNA were measured using real-time polymerase chain reaction (PCR) techniques.Measurement data was compared using t-test.Results When PC mRNA expressions in the blank group was as to 1,the expressions of PC1/3、PC2、furin、PC4 、PC5/6 、PACE4 and PC7/8 mRNA in HepG2.2.15 cells treated with 20μg/L TNF-α treatment for 18 h were all up-regulated,which were 3.3±0.7、79.3±3.3、77.5±1.3、19.2±3.1、1.3±0.1、1.4± 0.2、274.8± 7.1,respectively (all P＜0.05).Treatment of 20 μg/L recombinant TNF-α for 18 h significantly reduced core-associated HBV DNA compared with blank gourp (0.21∶1,t =8.79,P =0.002),while 20 μmol/L DEC significantly up-regulated core-associated HBV DNA (3.84∶ 1,t=7.67,P=0.004).Moreover,core-associated HBV DNA in group of DEC and TNF-α treatment was significantly higher than group of TNF-α treatment (0.31∶0.21,t=10.49,P=0.007).Conclusion Up-regulated PC mRNA expression induced by TNF-α is significantly associated with the inhibition of HBV replication.
