中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2014年
4期
199-203
,共5页
顾绍庆%李文静%韦苇%马晓蒙%Rupa Rana Shahi%赵媛
顧紹慶%李文靜%韋葦%馬曉矇%Rupa Rana Shahi%趙媛
고소경%리문정%위위%마효몽%Rupa Rana Shahi%조원
巨细胞病毒,人%杀伤细胞,天然%受体,细胞表面
巨細胞病毒,人%殺傷細胞,天然%受體,細胞錶麵
거세포병독,인%살상세포,천연%수체,세포표면
Cytomegalovirus,human%Killer cells,natural%Receptors,cell surface
目的 观察人CMV对NK细胞表面自然杀伤细胞2族(NKG2)成员,包括NKG2A、NKG2C和NKG2D表达的影响.方法 免疫磁珠法提取健康人PBMC中的NK细胞,与感染人CMV的人胚肺成纤维细胞(HELF)共培养24 h;同时设未感染CMV的HELF与NK细胞共培养组和NK细胞组.流式细胞技术检测各组NK细胞NKG2A、NKG2C和NKG2D的表达.组间比较采用Wilcoxon检验.结果 感染CMV的HELF+ NK细胞共培养组NKG2C和NKG2D表达水平为34.26％±7.99％和24.94％±5.24％,高于HELF+NK细胞共培养组的26.31％±6.41％(Z=-3.285,P=0.001)和20.02％±6.80％(Z=-3.285,P=0.001),也高于NK细胞组的25.78％±6.62％(Z=-3.211,P=0.001)和20.12％±6.75％(Z=-3.285,P=0.001);而HELF+NK细胞共培养组与NK细胞组比较差异无统计学意义(均P＞0.05).NKG2A在各组间表达差异亦无统计学意义(均P＞0.05).感染CMV的HELF+NK细胞共培养组NKG2A+ NKG2C-为2.99％±2.62％,NKG2A+NKG2D-为6.13％±2.44％,NKG2C+ NKG2A-为26.62％±7.63％,NKG2D+ NKG2A为20.44％±5.68％,而HELF+ NK细胞组分别为4.44％±4.25％(Z=-2.240,P=0.025)、6.99％±2.33％(Z=-2.053,P=0.040)、19.52％±6.80％(Z=-2.800,P=0.005)和15.78％±7.48％(Z=-2.875,P=0.004).NKG2A+ NKG2C+和NKG2A+ NKG2D+在各组间比较差异均无统计学意义(均P＞0.05).结论 人CMV感染可改变NK细胞受体的表达量,使NKG2C、NKG2D转导的活化性信号增强,NKG2A转导的抑制性信号减弱.
目的 觀察人CMV對NK細胞錶麵自然殺傷細胞2族(NKG2)成員,包括NKG2A、NKG2C和NKG2D錶達的影響.方法 免疫磁珠法提取健康人PBMC中的NK細胞,與感染人CMV的人胚肺成纖維細胞(HELF)共培養24 h;同時設未感染CMV的HELF與NK細胞共培養組和NK細胞組.流式細胞技術檢測各組NK細胞NKG2A、NKG2C和NKG2D的錶達.組間比較採用Wilcoxon檢驗.結果 感染CMV的HELF+ NK細胞共培養組NKG2C和NKG2D錶達水平為34.26％±7.99％和24.94％±5.24％,高于HELF+NK細胞共培養組的26.31％±6.41％(Z=-3.285,P=0.001)和20.02％±6.80％(Z=-3.285,P=0.001),也高于NK細胞組的25.78％±6.62％(Z=-3.211,P=0.001)和20.12％±6.75％(Z=-3.285,P=0.001);而HELF+NK細胞共培養組與NK細胞組比較差異無統計學意義(均P＞0.05).NKG2A在各組間錶達差異亦無統計學意義(均P＞0.05).感染CMV的HELF+NK細胞共培養組NKG2A+ NKG2C-為2.99％±2.62％,NKG2A+NKG2D-為6.13％±2.44％,NKG2C+ NKG2A-為26.62％±7.63％,NKG2D+ NKG2A為20.44％±5.68％,而HELF+ NK細胞組分彆為4.44％±4.25％(Z=-2.240,P=0.025)、6.99％±2.33％(Z=-2.053,P=0.040)、19.52％±6.80％(Z=-2.800,P=0.005)和15.78％±7.48％(Z=-2.875,P=0.004).NKG2A+ NKG2C+和NKG2A+ NKG2D+在各組間比較差異均無統計學意義(均P＞0.05).結論 人CMV感染可改變NK細胞受體的錶達量,使NKG2C、NKG2D轉導的活化性信號增彊,NKG2A轉導的抑製性信號減弱.
목적 관찰인CMV대NK세포표면자연살상세포2족(NKG2)성원,포괄NKG2A、NKG2C화NKG2D표체적영향.방법 면역자주법제취건강인PBMC중적NK세포,여감염인CMV적인배폐성섬유세포(HELF)공배양24 h;동시설미감염CMV적HELF여NK세포공배양조화NK세포조.류식세포기술검측각조NK세포NKG2A、NKG2C화NKG2D적표체.조간비교채용Wilcoxon검험.결과 감염CMV적HELF+ NK세포공배양조NKG2C화NKG2D표체수평위34.26％±7.99％화24.94％±5.24％,고우HELF+NK세포공배양조적26.31％±6.41％(Z=-3.285,P=0.001)화20.02％±6.80％(Z=-3.285,P=0.001),야고우NK세포조적25.78％±6.62％(Z=-3.211,P=0.001)화20.12％±6.75％(Z=-3.285,P=0.001);이HELF+NK세포공배양조여NK세포조비교차이무통계학의의(균P＞0.05).NKG2A재각조간표체차이역무통계학의의(균P＞0.05).감염CMV적HELF+NK세포공배양조NKG2A+ NKG2C-위2.99％±2.62％,NKG2A+NKG2D-위6.13％±2.44％,NKG2C+ NKG2A-위26.62％±7.63％,NKG2D+ NKG2A위20.44％±5.68％,이HELF+ NK세포조분별위4.44％±4.25％(Z=-2.240,P=0.025)、6.99％±2.33％(Z=-2.053,P=0.040)、19.52％±6.80％(Z=-2.800,P=0.005)화15.78％±7.48％(Z=-2.875,P=0.004).NKG2A+ NKG2C+화NKG2A+ NKG2D+재각조간비교차이균무통계학의의(균P＞0.05).결론 인CMV감염가개변NK세포수체적표체량,사NKG2C、NKG2D전도적활화성신호증강,NKG2A전도적억제성신호감약.
Objective To examine the effect of human cytomegalovirus (CMV) on the expressions of natural killer group 2 members (NKG2),including natural-killer group 2-member A (NKG2A),natural-killer group 2-member C (NKG2C) and natural-killer group 2 member D (NKG2D) receptors on the natural killer (NK) cells.Methods NK cells were isolated from the peripheral blood mononuclear cells of 20 healthy individuals using EasySep magnetic beads.NK cells and CMV-infected human embryonic lung fibroblast (HELF) were co-cultured for 24 h and regarded as experimental group.While CMV-uninfected NK cells co-cultured with HELF and only NK cells were taken as control groups.Flow cytometry was used to detect the expressions of the NK cell receptors in experimental and control groups.The comparison between groups was done by Wilcoxon rank sum test.Results The percentages of NK cells expressing NKG2C and NKG2D in group of CMV-infected NK cells co-cultured with HELF were 34.26％±7.99％ and 24.94％ ± 5.24％,respectively,which were significantly higher than those in CMVuninfected NK cells co cultured with HELF (26.31％ ±6.41％; Z=-3.285,P=0.001 and 20.02％±6.80％ ; Z=-3.285,P=0.001,respectively) and NK cells alone (25.78％± 6.62％ ; Z=-3.211,P=0.001 and 20.12 ％ ± 6.75 ％ ; Z=-3.285,P =0.001,respectively),while no significant changes were found in the expressions of the two receptors between groups of CMV-uninfected NK cells co-cultured with HELF and NK cells alone (both P＞0.05).Moreover,no significant changes were found in the expression of NKG2A among groups (all P＞0.05).The levels of NKG2A+ NKG2C,NKG2A+NKG2D,NKG2C+ NKG2A-and NKG2D+ NKG2A-cells in CMV infected NK cells co-cultured with HELFgroup were 2.99％ ±2.62％,6.13％±2.44％,26.62％ ±7.63％ and 20.44％±5.68％,respectively,while those in group of CMV-uninfected NK cells co-cultured with HELF were 4.44％ ± 4.25％ (Z=-2.240,P=0.025),6.99％±2.33％ (Z=-2.053,P=0.040),19.52％±6.80％ (Z=-2.800,P=0.005) and 15.78％±7.48％ (Z=-2.875,P=0.004),respectively.However,no significant changes were found in the expressions of NKG2A+ NKG2C+ and NKG2A+ NKG2D+ among groups (all P＞0.05).Conclusions CMV infection may change the NK receptor expressions,then stimulatory NKG2C and NKG2D signal transductions are enhanced and the inhibitory NKG2A signal conduction is weakened during CMV infection.
