中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2014年
4期
209-213
,共5页
莫瑞东%项晓刚%王芃%赖荣陶%赵钢德%刘昱含%周慧娟%郭斯敏%韩艳
莫瑞東%項曉剛%王芃%賴榮陶%趙鋼德%劉昱含%週慧娟%郭斯敏%韓豔
막서동%항효강%왕봉%뢰영도%조강덕%류욱함%주혜연%곽사민%한염
T淋巴细胞,辅助诱导%Th22细胞%白细胞介素22%肝炎,乙型,慢性%肝功能衰竭
T淋巴細胞,輔助誘導%Th22細胞%白細胞介素22%肝炎,乙型,慢性%肝功能衰竭
T림파세포,보조유도%Th22세포%백세포개소22%간염,을형,만성%간공능쇠갈
T-Lymphocytes,helper-inducer%Th22 cells%Interleukin-22%Hepatitis B,chronic%Liver failure
目的 探讨Th1 、Th17和Th22及其效应分子γ干扰素、IL-17和IL-22在CHB活动期及慢加急性肝衰竭(ACLF)中的作用.方法 上海交通大学医学院附属瑞金医院感染科住院的CHB患者45例,ACLF患者38例,同时收集健康志愿者(HC)26名.采集患者及健康志愿者新鲜全血,体外刺激培养后细胞表面及胞内染色,行流式细胞分析;同时收集受试者血浆,ELISA检测血浆IL-22等细胞因子表达.两组比较采用非配对t检验,相关性分析采用Spearman检验.结果 CHB组和ACLF组患者Th1频数分别为18.17％±9.01％和15.49％±9.06％,较健康对照组的22.45％±7.90％明显降低(t值分别为2.017和3.175,P值分别为0.048和0.002).ACLF组Th17频数为2.96％±1.30％,健康对照组为2.04％±0.91％,两组比较差异有统计学意义(t=3.121,P=0.003);同时ACLF组与CHB组比较差异亦有统计学意义(t=2.875,P=0.005).CHB组和ACLF组患者Th22频数较健康对照组明显升高(t值分别为2.128和3.995,P值分别为0.037和0.000).CHB组患者血浆γ干扰素水平为(25.65±10.18) pg/mL,IL-22水平为(24.06±13.11) pg/mL,明显高于健康对照组的(12.95±6.63) pg/mL和(15.08±8.51) pg/mL(t值分别为5.390和2.961,P值分别为0.000和0.004).ACLF组患者血浆IL-17和IL-22水平与健康对照组比较差异均有统计学意义(t值分别为2.654和4.123,P值分别为0.011和0.000).IL-22水平与外周血中Th22频数呈正相关(r=0.549,P=0.012).CHB组Th1频数与A LT、AST呈负相关(r值分别为-0.300和-0.384,P值分别为0.045和0.009).ACLF组患者Th22频数与终末期肝病模型评分呈正相关(r=0.345,P=0.034).结论 Th22和IL-22可能在CHB活动期和ACLF中起重要作用,深入研究Th22和IL-22在CHB和ACLF中的作用机制及其与疾病预后转归的关系,具有重要意义.
目的 探討Th1 、Th17和Th22及其效應分子γ榦擾素、IL-17和IL-22在CHB活動期及慢加急性肝衰竭(ACLF)中的作用.方法 上海交通大學醫學院附屬瑞金醫院感染科住院的CHB患者45例,ACLF患者38例,同時收集健康誌願者(HC)26名.採集患者及健康誌願者新鮮全血,體外刺激培養後細胞錶麵及胞內染色,行流式細胞分析;同時收集受試者血漿,ELISA檢測血漿IL-22等細胞因子錶達.兩組比較採用非配對t檢驗,相關性分析採用Spearman檢驗.結果 CHB組和ACLF組患者Th1頻數分彆為18.17％±9.01％和15.49％±9.06％,較健康對照組的22.45％±7.90％明顯降低(t值分彆為2.017和3.175,P值分彆為0.048和0.002).ACLF組Th17頻數為2.96％±1.30％,健康對照組為2.04％±0.91％,兩組比較差異有統計學意義(t=3.121,P=0.003);同時ACLF組與CHB組比較差異亦有統計學意義(t=2.875,P=0.005).CHB組和ACLF組患者Th22頻數較健康對照組明顯升高(t值分彆為2.128和3.995,P值分彆為0.037和0.000).CHB組患者血漿γ榦擾素水平為(25.65±10.18) pg/mL,IL-22水平為(24.06±13.11) pg/mL,明顯高于健康對照組的(12.95±6.63) pg/mL和(15.08±8.51) pg/mL(t值分彆為5.390和2.961,P值分彆為0.000和0.004).ACLF組患者血漿IL-17和IL-22水平與健康對照組比較差異均有統計學意義(t值分彆為2.654和4.123,P值分彆為0.011和0.000).IL-22水平與外週血中Th22頻數呈正相關(r=0.549,P=0.012).CHB組Th1頻數與A LT、AST呈負相關(r值分彆為-0.300和-0.384,P值分彆為0.045和0.009).ACLF組患者Th22頻數與終末期肝病模型評分呈正相關(r=0.345,P=0.034).結論 Th22和IL-22可能在CHB活動期和ACLF中起重要作用,深入研究Th22和IL-22在CHB和ACLF中的作用機製及其與疾病預後轉歸的關繫,具有重要意義.
목적 탐토Th1 、Th17화Th22급기효응분자γ간우소、IL-17화IL-22재CHB활동기급만가급성간쇠갈(ACLF)중적작용.방법 상해교통대학의학원부속서금의원감염과주원적CHB환자45례,ACLF환자38례,동시수집건강지원자(HC)26명.채집환자급건강지원자신선전혈,체외자격배양후세포표면급포내염색,행류식세포분석;동시수집수시자혈장,ELISA검측혈장IL-22등세포인자표체.량조비교채용비배대t검험,상관성분석채용Spearman검험.결과 CHB조화ACLF조환자Th1빈수분별위18.17％±9.01％화15.49％±9.06％,교건강대조조적22.45％±7.90％명현강저(t치분별위2.017화3.175,P치분별위0.048화0.002).ACLF조Th17빈수위2.96％±1.30％,건강대조조위2.04％±0.91％,량조비교차이유통계학의의(t=3.121,P=0.003);동시ACLF조여CHB조비교차이역유통계학의의(t=2.875,P=0.005).CHB조화ACLF조환자Th22빈수교건강대조조명현승고(t치분별위2.128화3.995,P치분별위0.037화0.000).CHB조환자혈장γ간우소수평위(25.65±10.18) pg/mL,IL-22수평위(24.06±13.11) pg/mL,명현고우건강대조조적(12.95±6.63) pg/mL화(15.08±8.51) pg/mL(t치분별위5.390화2.961,P치분별위0.000화0.004).ACLF조환자혈장IL-17화IL-22수평여건강대조조비교차이균유통계학의의(t치분별위2.654화4.123,P치분별위0.011화0.000).IL-22수평여외주혈중Th22빈수정정상관(r=0.549,P=0.012).CHB조Th1빈수여A LT、AST정부상관(r치분별위-0.300화-0.384,P치분별위0.045화0.009).ACLF조환자Th22빈수여종말기간병모형평분정정상관(r=0.345,P=0.034).결론 Th22화IL-22가능재CHB활동기화ACLF중기중요작용,심입연구Th22화IL-22재CHB화ACLF중적작용궤제급기여질병예후전귀적관계,구유중요의의.
Objective To investigate the role of helper T cell (Th)1,Th17 and Th22 and their effector molecules including interferon-gamma,interleukin (IL)-17 and IL-22 in active phase of chronic hepatitis B (CHB) and acute-on-chronic liver failure (ACLF).Methods Forty-five CHB patients and 38 ACLF patients at Ruijin Hospital of Shanghai Jiaotong University School of Medicine were included,and 26 healthy controls (HC) were also enrolled.Fresh heparinized peripheral blood was obtained and stimulated in vitro with the phorbol myristate acetate and ionomycin.Surface and intracellular staining were performed,then detected by flow cytometry.Plasma was also collected from all subjects for the detection of cytokine concentration such as IL-22 by enzyme-linked immunosorbent assay.Difference between two groups was analyzed by unpaired t test.Spearman correlation test was used for correlation analysis.Results The Th1 frequencies in CHB and ACLF groups were 18.17 ％ ±9.01 ％ and 15.49 ％ ± 9.06％,respectively,which were both significantly decreased compared with HC group (22.45％ ± 7.90％; t=2.017,P=0.048 andt=3.175,P=0.002,respectively).The frequency of Th17 in ACLF patients (2.96％±1.30％) was significantly increased compared with HC (2.04％±0.91％)(t=3.121,P=0.003) and CHB group (t=2.875,P =0.005).Both ACLF group and CHB group had significant higher frequencies of Th22 than HC (t=2.128,P=0.037 and t=3.995,P=0.000,respectively).The level of interferon-gamma was (25.65 ± 10.18) pg/mL and IL-22 was (24.06 ± 13.11) pg/mL in CHB patients,which were both increased significantly compared with that in HC ([12.95±6.63] pg/mL,t=5.390,P=0.000 and [15.08±8.51] pg/mL,t=2.961,P=0.004,respectively).And the plasma levels of IL-17 and IL-22 in ACLF patients were also significantly increased compared with that in HC (t=2.654,P=0.011 and t =4.123,P=0.000,respectively).The level of IL-22 in plasma was positively correlated with the frequency of Th22 in ACLF patients (r =0.549,P =0.012).In addition,the frequency of Th1 was negatively correlated with alanine aminotransferase or aspartate aminotransferase in CHBgroup (r=-0.300,P=0.045 and r=-0.384,P=0.009,respectively).The frequency of Th22 was positively correlated with the model for end stage liver disease score of ACLF patients (r=0.345,P=0.034).Conclusions Th22 and IL-22 may play a role in active phase of CHB and ACLF.Thus,it is of great importance to investigate how Th22 and IL-22 affect the disease activation and aggravation,and further to determine whether Th22 and IL-22 could impact the prognosis of the disease.
