中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2014年
8期
455-459
,共5页
胡燕%王宏萍%张璐%胡芸文%周晓明
鬍燕%王宏萍%張璐%鬍蕓文%週曉明
호연%왕굉평%장로%호예문%주효명
呼吸道感染%副流感病毒3型,人%HN基因%系统进化分析
呼吸道感染%副流感病毒3型,人%HN基因%繫統進化分析
호흡도감염%부류감병독3형,인%HN기인%계통진화분석
Respiratory tract infections%Parainfluenza virus 3,human%HN gene%Phylogenetic analysis
目的 了解3型副流感病毒(HPIV-3)的流行特点,以期为制定防制措施提供科学依据.方法 2009年6月到2010年6月上海交通大学医学院附属新华医院送上海市公共卫生临床中心检测的急性呼吸道感染患儿的鼻咽分泌物标本164份,反转录PCR法检测标本中的HPIV-3.对5份阳性标本的血凝素-神经氨酸酶(HN)基因编码区全长(1 719 bp)进行基因序列测定和进化分析.两组间比较采用精确卡方检验(双侧).结果 164份急性呼吸道感染患儿标本中HPIV阳性数为70份,其中HPIV-3阳性23份,阳性率为32.86%.感染多发生于春、夏季,且多数HPIV-3感染发生在13~36个月的患儿中.基于HN基因建立的系统进化树将5株上海分离株和36株来自不同国家和地区的参考株序列分成3个进化群(A、B和C).5株上海株和5株北京分离株归属于C3a群,上海株之间核苷酸和氨基酸的同源性分别为99.0%~99.5%和99.7%~100.0%.结论 HPIV-3在此次急性呼吸道感染患儿中所占比例较高,株系主要为C3a群,其进化很可能具有区域相关性.
目的 瞭解3型副流感病毒(HPIV-3)的流行特點,以期為製定防製措施提供科學依據.方法 2009年6月到2010年6月上海交通大學醫學院附屬新華醫院送上海市公共衛生臨床中心檢測的急性呼吸道感染患兒的鼻嚥分泌物標本164份,反轉錄PCR法檢測標本中的HPIV-3.對5份暘性標本的血凝素-神經氨痠酶(HN)基因編碼區全長(1 719 bp)進行基因序列測定和進化分析.兩組間比較採用精確卡方檢驗(雙側).結果 164份急性呼吸道感染患兒標本中HPIV暘性數為70份,其中HPIV-3暘性23份,暘性率為32.86%.感染多髮生于春、夏季,且多數HPIV-3感染髮生在13~36箇月的患兒中.基于HN基因建立的繫統進化樹將5株上海分離株和36株來自不同國傢和地區的參攷株序列分成3箇進化群(A、B和C).5株上海株和5株北京分離株歸屬于C3a群,上海株之間覈苷痠和氨基痠的同源性分彆為99.0%~99.5%和99.7%~100.0%.結論 HPIV-3在此次急性呼吸道感染患兒中所佔比例較高,株繫主要為C3a群,其進化很可能具有區域相關性.
목적 료해3형부류감병독(HPIV-3)적류행특점,이기위제정방제조시제공과학의거.방법 2009년6월도2010년6월상해교통대학의학원부속신화의원송상해시공공위생림상중심검측적급성호흡도감염환인적비인분비물표본164빈,반전록PCR법검측표본중적HPIV-3.대5빈양성표본적혈응소-신경안산매(HN)기인편마구전장(1 719 bp)진행기인서렬측정화진화분석.량조간비교채용정학잡방검험(쌍측).결과 164빈급성호흡도감염환인표본중HPIV양성수위70빈,기중HPIV-3양성23빈,양성솔위32.86%.감염다발생우춘、하계,차다수HPIV-3감염발생재13~36개월적환인중.기우HN기인건립적계통진화수장5주상해분리주화36주래자불동국가화지구적삼고주서렬분성3개진화군(A、B화C).5주상해주화5주북경분리주귀속우C3a군,상해주지간핵감산화안기산적동원성분별위99.0%~99.5%화99.7%~100.0%.결론 HPIV-3재차차급성호흡도감염환인중소점비례교고,주계주요위C3a군,기진화흔가능구유구역상관성.
Objective To understand the epidemiologic feature of human parainfluenza virus type 3 (HPIV-3) in Shanghai,and to provide scientific evidence for formulating prevention and control measures in the future.Methods A total of 164 nasopharyngeal aspirates samples taken from children with acute respiratory infection (ARI) were collected from Xinhua Hospital Affiliated to Shanghai Jiaotong University and sent to Shanghai Public Health Clinical Center from June 2009 to June 2010.Samples were detected for HPIV-3 by reversed transcription-polymerase chain reaction (RT-PCR).Full-length hemagglutininneuraminidase (HN) gene (1 719 bp) of five positive samples were sequenced for phylogenetic analysis.Comparison between two groups was evaluated by the precise chi-square test (two sided).Results Of 164 samples,70 samples were infected with parainfluenza virus,and HPIV-3 was detected positive in 23 samples with the positive rate of 32.86%.HPIV-3 infections were most common in spring and summer,and most of infections were mainly found in 13-36 month-old infants.Five Shanghai isolates and 36 reference sequences from different countries and areas were divided by HN gene-based phylogenetic tree into three clusters (A,B and C).Five Shanghai isolates and five Beijing isolates belonged to C3a group.The homologies of nucleotide and amino acid sequences between five Shanghai isolates and five Beijing isolates were 99.0%-99.5% and 99.7%-100.0%,respectively.Conclusions HPIV-3 accounts for a high proportion in children with ARI in Shanghai.C3a group may be the main lineage of HPIV-3,which suggests that HPIV-3 may be of regionally correlation.