CAJ | 학술논문

目的利用组织工程技术体外构建组织工程化神经复合物,对大鼠损伤脊髓进行修复并观察其效果. 方法 (1)分离培养大鼠神经干细胞(neural stem cells,NSCs),制备改性胶原支架,体外构建NSCs/改性胶原支架复合物,以胚胎脊髓提取液对NSCs进行诱导分化,通过组织化学检测和扫描电镜观察其结构.(2)制作大鼠脊髓半横断损伤模型,植入组织工程化神经复合物,术后每2周进行功能学检查,并于术后3周及8周进行组织学检查以评估修复脊髓损伤(spinal cord injury,SCI的效果. 结果改性胶原支架构建的组织工程化神经复合物移植至大鼠体内后,其种子细胞存活并进一步分化,形成功能活跃的神经元,分化的胶质细胞形成规则的网状结构.大鼠损伤脊髓平面以下运动功能获得了较明显的恢复. 结论以改性胶原为支架的组织工程化神经复合物对大鼠损伤脊髓的结构重建和功能恢复都有一定的作用.
목적 이용조직공정기술체외구건조직공정화신경복합물,대대서손상척수진행수복병관찰기효과. 방법 (1)분리배양대서신경간세포(neural stem cells,NSCs),제비개성효원지가,체외구건NSCs/개성효원지가복합물,이배태척수제취액대NSCs진행유도분화,통과조직화학검측화소묘전경관찰기결구.(2)제작대서척수반횡단손상모형,식입조직공정화신경복합물,술후매2주진행공능학검사,병우술후3주급8주진행조직학검사이평고수복척수손상(spinal cord injury,SCI적효과. 결과 개성효원지가구건적조직공정화신경복합물이식지대서체내후,기충자세포존활병진일보분화,형성공능활약적신경원,분화적효질세포형성규칙적망상결구.대서손상척수평면이하운동공능획득료교명현적회복. 결론 이개성효원위지가적조직공정화신경복합물대대서손상척수적결구중건화공능회복도유일정적작용.
Objective To repair the injured spinal cord in adult rats using tissue-engineered neural complex constructed in vitro by tissue engineering techniques and discuss the treatment effect.Methods Neural stem cells (NSCs) from rats were cultured separately and modified collagen scaffold was also prepared to construct complex of NSCs /modified collagen scaffold in vitro.Embryo spine cord extract was administered to induce differentiation of NSCs,the structure of which was revealed by histochemical technique and scanning electron microscope.The rat models of hemisected spinal cord injury were built and grafted with tissue-engineered neural complex.Functional recovery of the models was evaluated every two weeks postoperatively.Effect in repair of spinal cord injury was assessed by histological method at postoperative three and eight weeks.Results After tissue-engineered neural complex planted in the modified collagen scaffold was grafted into the injured spinal cord in adult rats,the seed cells survived and further differentiated into functionally active neurons.The differentiated glia cells presented quite regular reticular structure.Motor function below the level of the injured spinal cord in adult rats obtained obvious recovery.Conclusion Tissue-engineered neural complex seeded in the modified collagen scaffold takes effect in structural reconstruction and functional recovery of the injured spinal cord in adult rats.