中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2013年
4期
359-363
,共5页
张海宁%王英振%吕成昱%周峰%续宗耀
張海寧%王英振%呂成昱%週峰%續宗耀
장해저%왕영진%려성욱%주봉%속종요
半月板,胫骨%基因%组织工程%胰岛素样生长因子Ⅰ
半月闆,脛骨%基因%組織工程%胰島素樣生長因子Ⅰ
반월판,경골%기인%조직공정%이도소양생장인자Ⅰ
Menisci,tibial%Genes%Tissue engineering%Insulin-like growth factor Ⅰ
目的 建立全层无血运区半月板缺损模型,观察人胰岛素样生长因子Ⅰ (human insulin-like growth factor Ⅰ,hIGF-Ⅰ)基因修饰骨髓间充质干细胞(bone-marrow mesenchymal stem cells,BMSCs)复合可注射藻酸钙凝胶修复半月板缺损的效果. 方法 制造成年山羊半月板前角无血运区全层缺损模型.实验分为四组,即基因增强组织工程(gene-enhanced tissue engineering,GETE)组(GETE组):用hIGF-Ⅰ基因转染的BMSCs复合可注射藻酸钙凝胶修复半月板缺损;BMSCs组:用BMSCs复合藻酸钙凝胶修复缺损;空载体组:用藻酸钙凝胶空载体修复缺损;对照组:缺损旷置,不做任何修复.术后4,8,16周时相点行大体观察;光镜、电镜观察修复组织变化;测定修复组织中蛋白聚糖含量. 结果 GETE组修复半月板缺损在4~ 16周效果逐渐改善,缺损完全被修复组织填充,色白质韧,结合紧密,与正常半月板组织相似,大体观察优于其他各组;光镜下可见细胞随凝胶纤维排列分布,载体纤维间隙大多为细胞分泌基质所充填,细胞排列密集,基质分布均匀;扫描电镜观察到纤维排列规整紧密,纤维间缝隙为致密的细胞外基质填充;修复组织中蛋白聚糖含量较高,但与正常半月板仍有差异. 结论 转染hIGF-Ⅰ基因的BMSCs复合可注射藻酸钙凝胶可改善全层半月板缺损的修复效果.
目的 建立全層無血運區半月闆缺損模型,觀察人胰島素樣生長因子Ⅰ (human insulin-like growth factor Ⅰ,hIGF-Ⅰ)基因脩飾骨髓間充質榦細胞(bone-marrow mesenchymal stem cells,BMSCs)複閤可註射藻痠鈣凝膠脩複半月闆缺損的效果. 方法 製造成年山羊半月闆前角無血運區全層缺損模型.實驗分為四組,即基因增彊組織工程(gene-enhanced tissue engineering,GETE)組(GETE組):用hIGF-Ⅰ基因轉染的BMSCs複閤可註射藻痠鈣凝膠脩複半月闆缺損;BMSCs組:用BMSCs複閤藻痠鈣凝膠脩複缺損;空載體組:用藻痠鈣凝膠空載體脩複缺損;對照組:缺損曠置,不做任何脩複.術後4,8,16週時相點行大體觀察;光鏡、電鏡觀察脩複組織變化;測定脩複組織中蛋白聚糖含量. 結果 GETE組脩複半月闆缺損在4~ 16週效果逐漸改善,缺損完全被脩複組織填充,色白質韌,結閤緊密,與正常半月闆組織相似,大體觀察優于其他各組;光鏡下可見細胞隨凝膠纖維排列分佈,載體纖維間隙大多為細胞分泌基質所充填,細胞排列密集,基質分佈均勻;掃描電鏡觀察到纖維排列規整緊密,纖維間縫隙為緻密的細胞外基質填充;脩複組織中蛋白聚糖含量較高,但與正常半月闆仍有差異. 結論 轉染hIGF-Ⅰ基因的BMSCs複閤可註射藻痠鈣凝膠可改善全層半月闆缺損的脩複效果.
목적 건립전층무혈운구반월판결손모형,관찰인이도소양생장인자Ⅰ (human insulin-like growth factor Ⅰ,hIGF-Ⅰ)기인수식골수간충질간세포(bone-marrow mesenchymal stem cells,BMSCs)복합가주사조산개응효수복반월판결손적효과. 방법 제조성년산양반월판전각무혈운구전층결손모형.실험분위사조,즉기인증강조직공정(gene-enhanced tissue engineering,GETE)조(GETE조):용hIGF-Ⅰ기인전염적BMSCs복합가주사조산개응효수복반월판결손;BMSCs조:용BMSCs복합조산개응효수복결손;공재체조:용조산개응효공재체수복결손;대조조:결손광치,불주임하수복.술후4,8,16주시상점행대체관찰;광경、전경관찰수복조직변화;측정수복조직중단백취당함량. 결과 GETE조수복반월판결손재4~ 16주효과축점개선,결손완전피수복조직전충,색백질인,결합긴밀,여정상반월판조직상사,대체관찰우우기타각조;광경하가견세포수응효섬유배렬분포,재체섬유간극대다위세포분비기질소충전,세포배렬밀집,기질분포균균;소묘전경관찰도섬유배렬규정긴밀,섬유간봉극위치밀적세포외기질전충;수복조직중단백취당함량교고,단여정상반월판잉유차이. 결론 전염hIGF-Ⅰ기인적BMSCs복합가주사조산개응효가개선전층반월판결손적수복효과.
Objective To establish a model of full-thickness avascular meniscal defect to assess outcome of bone-marrow mesenchymal stem cells (BMSCs) modified with human insulin-like growth factor Ⅰ (hIGF-Ⅰ) gene and compounded with injectable calcium alginate gel in repair of meniscal defect.Methods Models of full-thickness defect were created in the anterior comer of meniscus in goats,an area lacking of blood supply.The trial categorized the models to four groups:gene-ehanced tissue engineering (GETE) group (hIGF-Ⅰ transfected BMSCs were mixed with calcium alginate gel),BMSCs group (BMSCs were mixed with calcium alginate gel),empty group (calcium alginate gel was used alone) and control group (the defect was excluded from repair).Macroscopy was done at 4,8,and 16 weeks after operation.Variation of repair tissue was observed by light and scanning electric microscopy and aggrecan in repair tissue was determined as well.Results Meniscal defect was on the mend at 4-16 weeks after operation in GETE group,with the defect area being thoroughly filled with the white,elastic and tight repair tissue similar to normal meniscal tissue.Macroscopic examination showed a better result in GETE group than that in other groups.Light microscopy showed that repair tissue which was mainly fibrochondrocytes was arranged in line with calcium alginate fibers and that space between the fibers was mostly crammed with the matrix secreted by those cells.At the same time,those cells were tightly arranged and the matrix secreted by those cells was equally distributed according to light microscopy.Electroscopy demonstrated neat and tight arrangement of fibers and tight extracellular matrix in fiber space in GETE group.Aggrecan concentration in GETE group was relatively higher than in other groups,but still had difference from the normal meniscus.Conclusion hIGF-Ⅰ gene-transfected BMSCs combined with injectable calcium alginate gel can improve the effect in repair of full-thickness meniscal defect.