中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2013年
9期
882-888
,共7页
陈云燕%王兴民%宋瑞%赵克森
陳雲燕%王興民%宋瑞%趙剋森
진운연%왕흥민%송서%조극삼
休克,出血性%血小板%线粒体
休剋,齣血性%血小闆%線粒體
휴극,출혈성%혈소판%선립체
Shock,hemorrhagic%Blood platelets%Mitochondria
目的 探讨重症失血性休克大鼠血小板线粒体变化程度与休克轻重程度的关系.方法 选择36只Wistar大鼠并按随机数字表法分为假手术组、休克30,60,120 min组、休克120 min+等渗盐水+输血组(NS组)及休克120 min+虎杖苷+输血组(PD组),每组6只.荧光素-荧光素酶试剂盒测定血小板三磷酸腺苷(adenosine triphosphate,ATP)含量；电镜观察血小板线粒体形态；Calcein-AM和CoCl2测定线粒体通透性转变孔状态；JC-1线粒体膜电位试剂盒探测线粒体膜电位(△Ψm)变化；过氧化脂质(lipid hydroperoxide,LPO)试剂盒检测血小板中LPO；吖啶橙(acridine orange,AO)检测血小板溶酶体稳定性. 结果 休克60 min组血小板ATP合成显著减少(P＜0.01),ATP含量随休克时间增加而减少,在NS组中减少最为明显[为正常值的(50.75±9.15)％].休克30 min组出现线粒体肿胀,嵴结构模糊消失,线粒体膜电位(△Ψm)下降及溶酶体稳定性下降(苍白细胞增多)；休克60 min组钙黄绿素荧光减弱(P＜0.01).PD组上述各指标都有所改善,特别是血小板ATP水平恢复到正常值的(79.57±8.48)％. 结论 重症休克30～60 min时发生血小板线粒体功能不全,血小板线粒体功能检测可作为重症休克诊断和治疗的一项非损伤性指标.
目的 探討重癥失血性休剋大鼠血小闆線粒體變化程度與休剋輕重程度的關繫.方法 選擇36隻Wistar大鼠併按隨機數字錶法分為假手術組、休剋30,60,120 min組、休剋120 min+等滲鹽水+輸血組(NS組)及休剋120 min+虎杖苷+輸血組(PD組),每組6隻.熒光素-熒光素酶試劑盒測定血小闆三燐痠腺苷(adenosine triphosphate,ATP)含量；電鏡觀察血小闆線粒體形態；Calcein-AM和CoCl2測定線粒體通透性轉變孔狀態；JC-1線粒體膜電位試劑盒探測線粒體膜電位(△Ψm)變化；過氧化脂質(lipid hydroperoxide,LPO)試劑盒檢測血小闆中LPO；吖啶橙(acridine orange,AO)檢測血小闆溶酶體穩定性. 結果 休剋60 min組血小闆ATP閤成顯著減少(P＜0.01),ATP含量隨休剋時間增加而減少,在NS組中減少最為明顯[為正常值的(50.75±9.15)％].休剋30 min組齣現線粒體腫脹,嵴結構模糊消失,線粒體膜電位(△Ψm)下降及溶酶體穩定性下降(蒼白細胞增多)；休剋60 min組鈣黃綠素熒光減弱(P＜0.01).PD組上述各指標都有所改善,特彆是血小闆ATP水平恢複到正常值的(79.57±8.48)％. 結論 重癥休剋30～60 min時髮生血小闆線粒體功能不全,血小闆線粒體功能檢測可作為重癥休剋診斷和治療的一項非損傷性指標.
목적 탐토중증실혈성휴극대서혈소판선립체변화정도여휴극경중정도적관계.방법 선택36지Wistar대서병안수궤수자표법분위가수술조、휴극30,60,120 min조、휴극120 min+등삼염수+수혈조(NS조)급휴극120 min+호장감+수혈조(PD조),매조6지.형광소-형광소매시제합측정혈소판삼린산선감(adenosine triphosphate,ATP)함량；전경관찰혈소판선립체형태；Calcein-AM화CoCl2측정선립체통투성전변공상태；JC-1선립체막전위시제합탐측선립체막전위(△Ψm)변화；과양화지질(lipid hydroperoxide,LPO)시제합검측혈소판중LPO；아정등(acridine orange,AO)검측혈소판용매체은정성. 결과 휴극60 min조혈소판ATP합성현저감소(P＜0.01),ATP함량수휴극시간증가이감소,재NS조중감소최위명현[위정상치적(50.75±9.15)％].휴극30 min조출현선립체종창,척결구모호소실,선립체막전위(△Ψm)하강급용매체은정성하강(창백세포증다)；휴극60 min조개황록소형광감약(P＜0.01).PD조상술각지표도유소개선,특별시혈소판ATP수평회복도정상치적(79.57±8.48)％. 결론 중증휴극30～60 min시발생혈소판선립체공능불전,혈소판선립체공능검측가작위중증휴극진단화치료적일항비손상성지표.
Objective To investigate relationship between the variation degree of platelet mitochondria in rats with severe hemorrhagic shock and the degree of shock.Methods Thirty-six Wistar rats were divided into sham group,shock 30,60,and 120 minutes groups,shock 120 minutes + normal saline (NS) + blood reinfusion group (NS group) and shock 120 minutes + polydatin (PD) + blood reinfusion group (PD group) according to random number table,with six rats per group.Content of ATP in platelets was detected by fluorescein-luciferase assay kit; structure of platelet mitochondria by electron microscope; state of mitochondrial permeability transition pore by Calcein-AM and CoCl2 ; change of mitochondrial membrane potential (△Ψm) by JC-1 mitochondrial membrane potential kit; lipid hydroperoxide (LPO) in platelets by LPO assay kit; stability of platelet lysosomes by acridine orange (AO).Results ATP released from platelets was reduced significantly in shock 60 minutes group (P ＜0.01) and with the prolong of shock period,further reduction was observed,particularly in NS group [(50.75 ± 9.15)％ of normal value].Mitochondrial swelling with poorly defined crista structure,declined △Ψ and low lysosome stability (pale cells were increased) were observed in shock 30 minutes group.Calcein fluorescence in mitochondria was faded in shock 60 minutes group (P ＜ 0.01).Whereas in PD group,all the above indices presented some recovery with ATP level returned to nearly (79.57 ± 8.48) ％ of normal value in particular.Conclusions Platelet mitochondrial dysfunction takes place at 30-60 minutes following severe shock.Hence,it may be served as an non-invasive index for the diagnose and treatment of severe shock.
