CAJ | 학술논문

目的探讨鲍曼不动杆菌的abaI基因表达对生物膜形成的影响. 方法以鲍曼不动杆菌标准菌株ATCC19606为对照,选择烧伤患者的鲍曼不动杆菌S,在6,24,48 h采用实时荧光定量PT-PCR检测基因abaI、pgaA、pgaB、pgaC的表达；生物感应器检测N-酰基-高丝氨酸内酯(N-acyl-homoserine lactones,AHLs)的分泌；MTT法检测生物膜形成变化. 结果 (1)6h时abaI、pgaA、pgaB、pgaC各基因的表达分别为8.63±5.93,1.98±1.93,1.01 ±1.32,2.67±3.46;24 h时各基因表达迅速增加,分别为22.81±17.60,5.13±4.32,5.66 ±3.97,11.97±7.75;48 h时各基因表达又迅速下降,分别为3.43±0.88,1.30±0.24,3.01±3.00,3.02±3.29.与24 h比较,6 h pgaB、pgaC的表达和48 h pgaA、pgaC的表达差异均有统计学意义(P＜0.05).(2) AHLs分泌在6h为18.49±11.03,24h时达到高峰为52.23±15.95,随后48 h下降为5.53±0.94,24 h与6,48 h比较差异有统计学意义(P＜0.05).(3)6h生物膜生成为0.49±0.11;24,48 h生物膜生成分别为2.83±0.44,2.71±0.15,较6h均显著增加(P＜0.05).(4) AHLs、生物膜及abaI、pgaA、pgaB、pgaC表达的相关性分析显示,abaI与pgaA、AHLs与pgaC呈正相关,差异有统计学意义(P＜0.05). 结论在生物膜形成过程中,鲍曼不动杆菌可能通过改变abaI基因表达、AHLs分泌调控生物膜形成相关基因pgaA、pgaC等的表达和生物膜形成以适应外部环境变化.
목적 탐토포만불동간균적abaI기인표체대생물막형성적영향. 방법 이포만불동간균표준균주ATCC19606위대조,선택소상환자적포만불동간균S,재6,24,48 h채용실시형광정량PT-PCR검측기인abaI、pgaA、pgaB、pgaC적표체；생물감응기검측N-선기-고사안산내지(N-acyl-homoserine lactones,AHLs)적분비；MTT법검측생물막형성변화. 결과 (1)6h시abaI、pgaA、pgaB、pgaC각기인적표체분별위8.63±5.93,1.98±1.93,1.01 ±1.32,2.67±3.46;24 h시각기인표체신속증가,분별위22.81±17.60,5.13±4.32,5.66 ±3.97,11.97±7.75;48 h시각기인표체우신속하강,분별위3.43±0.88,1.30±0.24,3.01±3.00,3.02±3.29.여24 h비교,6 h pgaB、pgaC적표체화48 h pgaA、pgaC적표체차이균유통계학의의(P＜0.05).(2) AHLs분비재6h위18.49±11.03,24h시체도고봉위52.23±15.95,수후48 h하강위5.53±0.94,24 h여6,48 h비교차이유통계학의의(P＜0.05).(3)6h생물막생성위0.49±0.11;24,48 h생물막생성분별위2.83±0.44,2.71±0.15,교6h균현저증가(P＜0.05).(4) AHLs、생물막급abaI、pgaA、pgaB、pgaC표체적상관성분석현시,abaI여pgaA、AHLs여pgaC정정상관,차이유통계학의의(P＜0.05). 결론 재생물막형성과정중,포만불동간균가능통과개변abaI기인표체、AHLs분비조공생물막형성상관기인pgaA、pgaC등적표체화생물막형성이괄응외부배경변화.
Objective To investigate the influence of abaI expression on acinetobacter baumannii biofilm formation.Methods Acinetobacter baumannii strain S isolated from bums patients was collected for the study,while the standard strain ATCC19606 was served as the control.At 6,24 and 48 hours,the gene expressions of abaI,pgaA,pgaB and pgaC were detected by real-time fluorescent quantitative PT-PCR,secretion of N-acyl-homoserine lactones (AHLs) by biological sensor and biofilm formation by MTT method.Results (1) Gene expressions of abaI,pgaA,pgaB and pgaC at 6 hours were 8.63 ±5.93,1.98 ± 1.93,1.01 ± 1.32 and 2.67 ± 3.46 respectively,which showed a quick increase at 24 hours (22.81 ± 17.60,5.13 ± 4.32,5.66 ± 3.97,11.97 ± 7.75 respectively),followed by a rapid decline in 48 hours (3.43 ± 0.88,1.30 ± 0.24,3.01 ± 3.00,3.02 ± 3.29 respectively).Gene expressions of pgaB and pgaC at 6 hours and that of pgaA and pgaC at 48 hours revealed statistically significant differences from those at 24 hours (P ＜ 0.05).(2) AHLs showed a level of 18.49 ± 11.03 at 6 hours,reached a peak of 52.23 ± 15.95 at 24 hours,then descended to 5.53 ± 0.94 at 48 hours.AHLs level at 24 hours showed statistically significant difference from that at 6 hours and 48 hours (P ＜ 0.05).(3)Biofilm formation at 24 hours and 48 hours was 2.83 ±0.44 and 2.71 ±0.15 respectively,far higher than that at 6 hours (0.49 ± 0.11,P ＜ 0.05).(4) In the correlation analysis among AHLs,biofilm formation and gene abaI,pgaA,pgaB and pgaC expressions,significant positive correlation was found between abaI and pgaA and between AHLs and pgaC expression (P ＜ 0.05).Conclusion Acinetobacter baumannii may regulate gene expressions of pgaA and pgaC responsible for biofilm formation to adjust to the external environment by means of changing abaI gene expression and AHLs secretion.