CAJ | 학술논문

目的探讨雪旺细胞(SCs)对β-磷酸三钙/骨髓基质干细胞诱导分化的成骨细胞(β-TCP/BDOB)组织工程骨修复大鼠股骨缺损的影响. 方法将SD大鼠骨髓基质于细胞(BMSCs)诱导分化为BDOB,并从SD乳鼠中提取SCs.将36只SD大鼠随机分为3组,每组12只,分别将术前预种植SCs的β-TCP/BDOB组织工程骨(A组)、β-TCP/BDOB组织工程骨(B组)、单纯β-TCP材料(C组)植入大鼠体内修复长度为8 mm的股骨缺损.于术后12周行X线摄片、组织学检查和显微CT扫描观察各组股骨缺损部位新生骨质的形成情况. 结果术后12周X线片和显微CT扫描结果显示:A组大鼠股骨缺损已完全愈合,B组大鼠股骨缺损部分愈合,C组大鼠股骨缺损未愈合.A组、B组、C组的X线影像学评分平均分别为(12.08 ±0.90)、(9.25±1.06)、(6.17±1.12)分,3组比较差异有统计学意义(F=99.553、P=0.000),3组间两两比较差异均有统计学意义(P＜0.05).组织学检查结果显示:A组植入物中有大量骨质形成,广泛分布肥大的软骨细胞与成骨细胞；B组植入物中也可见散在新生骨形成,软骨细胞与成骨细胞散在稀疏分布；C组植入物中新生骨质形成极少,几乎未见软骨细胞或成骨细胞. 结论 SCs可提高β-TCP/BDOB组织工程骨修复大鼠股骨缺损的效果.
목적 탐토설왕세포(SCs)대β-린산삼개/골수기질간세포유도분화적성골세포(β-TCP/BDOB)조직공정골수복대서고골결손적영향. 방법 장SD대서골수기질우세포(BMSCs)유도분화위BDOB,병종SD유서중제취SCs.장36지SD대서수궤분위3조,매조12지,분별장술전예충식SCs적β-TCP/BDOB조직공정골(A조)、β-TCP/BDOB조직공정골(B조)、단순β-TCP재료(C조)식입대서체내수복장도위8 mm적고골결손.우술후12주행X선섭편、조직학검사화현미CT소묘관찰각조고골결손부위신생골질적형성정황. 결과 술후12주X선편화현미CT소묘결과현시:A조대서고골결손이완전유합,B조대서고골결손부분유합,C조대서고골결손미유합.A조、B조、C조적X선영상학평분평균분별위(12.08 ±0.90)、(9.25±1.06)、(6.17±1.12)분,3조비교차이유통계학의의(F=99.553、P=0.000),3조간량량비교차이균유통계학의의(P＜0.05).조직학검사결과현시:A조식입물중유대량골질형성,엄범분포비대적연골세포여성골세포；B조식입물중야가견산재신생골형성,연골세포여성골세포산재희소분포；C조식입물중신생골질형성겁소,궤호미견연골세포혹성골세포. 결론 SCs가제고β-TCP/BDOB조직공정골수복대서고골결손적효과.
Objective To explore the effect of Schwann cells (SCs) on repair of femoral defects using a tissue engineering bone graft of β-tricalcium phosphate plus bone marrow stromal cell-derived osteoblast (β-TCP/BDOB) in a rat model.Methods Bone marrow stromal cells (BMSCs) collected from Sprague-Dawley (SD) rats were cultured and induced into BDOB in vitro.SCs were collected from neonatal SD rats.Thirty-six SD rats were randomly divided into 3 even groups (n =12).In group A,β-TCP/BDOB tissue engineering bone grafts loaded with SCs were implanted to repair the femoral defects of 8 mm.In groups B and C,β-TCP/BDOB tissue engineering bone grafts and β-TCP scaffolds were implanted without SCs into the 8 mm defects,respectively.X-ray detection,histological examination and Micro-CT detection were performed 12 weeks after the implantation to evaluate new bone formation.Results X-ray and Micro-CT detections indicated that the femoral defects were completely bridged in group A,partly repaired in group B and unhealed in group C.The X-ray score was 12.08 ± 0.90 for group A,9.25 ± 1.06 for group B and 6.17 ± 1.12 for group C,with a significant difference among the three (F=99.553,P=0.000) and between any 2 groups (P ＜ O.05).The histological examinations showed abundant ossified formation and a large number of hypertrophic osteoblasts and chondrocytes in the implanted tissue engineering bone grafts in group A,scattered ossification sites and sparse osteoblasts and chondrocytes in the implants in group B,few ossification sites and few osteoblasts and chondrocytes in group C.Conclusion SCs can improve the repairing effect of a β-TCP/BDOB tissue engineering bone graft on femoral defects in rats.