中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2013年
7期
597-602
,共6页
丁坦%郝虎萍%杜俊杰%罗卓荆
丁坦%郝虎萍%杜俊傑%囉卓荊
정탄%학호평%두준걸%라탁형
纳米复合物%神经再生%组织工程%周围神经%组织支架
納米複閤物%神經再生%組織工程%週圍神經%組織支架
납미복합물%신경재생%조직공정%주위신경%조직지가
Nanocomposites%Nerve regeneration%Tissue engineering%Peripheral nerve%Tissue scaffold
目的 探讨纳米银-胶原蛋白组织工程周围神经支架的构建及其理化性能和生物相容性.方法 采用不同浓度纳米银溶液构建含不同浓度纳米银(1、2、3、4、5、6 mg/L)的胶原蛋白组织工程支架,对照组仅加入不含纳米银的等量纯水.分别以扫描电镜观察其横截面和纵截面孔径结构,测定微拉伸强度.采用5只SD仔鼠进行纳米银-胶原蛋白支架细胞毒性实验,8只雄性成年SD大鼠进行支架体内降解实验,12只雄性成年SD大鼠行纳米银体内蓄积毒性测试.逐步按照空间结构以及在模拟体内环境下的多种理化特性筛选最佳纳米银含量的纳米银-胶原蛋白材料,构建周围神经支架.结果 纳米银含量为l、2 mg/L的纳米银-胶原蛋白支架内部孔径结构高度仿生,其中2 mg/L纳米银含量组的支架抗拉伸强度达到0.22 MPa,优于其他测试组.采用含2 mg/L纳米银支架材料制作薄膜行雪旺细胞培养证实该支架材料无神经细胞毒性.体内降解实验显示该组织工程支架从植入体内开始缓慢发生降解,4个月后可以完全降解.纳米银体内蓄积毒性测试显示在支架植入体内直至完全降解过程中,脑、脊髓、肝和肾脏银元素含量均低于l ng/mL,不存在蓄积毒性.结论 使用含2mg/L纳米银的胶原蛋白周围神经组织工程支架不仅在内部空间结构上可高度仿生周围神经,且具有较好的抗拉伸强度、在体内可完全降解、无细胞毒性和银蓄积毒性等优点.
目的 探討納米銀-膠原蛋白組織工程週圍神經支架的構建及其理化性能和生物相容性.方法 採用不同濃度納米銀溶液構建含不同濃度納米銀(1、2、3、4、5、6 mg/L)的膠原蛋白組織工程支架,對照組僅加入不含納米銀的等量純水.分彆以掃描電鏡觀察其橫截麵和縱截麵孔徑結構,測定微拉伸彊度.採用5隻SD仔鼠進行納米銀-膠原蛋白支架細胞毒性實驗,8隻雄性成年SD大鼠進行支架體內降解實驗,12隻雄性成年SD大鼠行納米銀體內蓄積毒性測試.逐步按照空間結構以及在模擬體內環境下的多種理化特性篩選最佳納米銀含量的納米銀-膠原蛋白材料,構建週圍神經支架.結果 納米銀含量為l、2 mg/L的納米銀-膠原蛋白支架內部孔徑結構高度倣生,其中2 mg/L納米銀含量組的支架抗拉伸彊度達到0.22 MPa,優于其他測試組.採用含2 mg/L納米銀支架材料製作薄膜行雪旺細胞培養證實該支架材料無神經細胞毒性.體內降解實驗顯示該組織工程支架從植入體內開始緩慢髮生降解,4箇月後可以完全降解.納米銀體內蓄積毒性測試顯示在支架植入體內直至完全降解過程中,腦、脊髓、肝和腎髒銀元素含量均低于l ng/mL,不存在蓄積毒性.結論 使用含2mg/L納米銀的膠原蛋白週圍神經組織工程支架不僅在內部空間結構上可高度倣生週圍神經,且具有較好的抗拉伸彊度、在體內可完全降解、無細胞毒性和銀蓄積毒性等優點.
목적 탐토납미은-효원단백조직공정주위신경지가적구건급기이화성능화생물상용성.방법 채용불동농도납미은용액구건함불동농도납미은(1、2、3、4、5、6 mg/L)적효원단백조직공정지가,대조조부가입불함납미은적등량순수.분별이소묘전경관찰기횡절면화종절면공경결구,측정미랍신강도.채용5지SD자서진행납미은-효원단백지가세포독성실험,8지웅성성년SD대서진행지가체내강해실험,12지웅성성년SD대서행납미은체내축적독성측시.축보안조공간결구이급재모의체내배경하적다충이화특성사선최가납미은함량적납미은-효원단백재료,구건주위신경지가.결과 납미은함량위l、2 mg/L적납미은-효원단백지가내부공경결구고도방생,기중2 mg/L납미은함량조적지가항랍신강도체도0.22 MPa,우우기타측시조.채용함2 mg/L납미은지가재료제작박막행설왕세포배양증실해지가재료무신경세포독성.체내강해실험현시해조직공정지가종식입체내개시완만발생강해,4개월후가이완전강해.납미은체내축적독성측시현시재지가식입체내직지완전강해과정중,뇌、척수、간화신장은원소함량균저우l ng/mL,불존재축적독성.결론 사용함2mg/L납미은적효원단백주위신경조직공정지가불부재내부공간결구상가고도방생주위신경,차구유교호적항랍신강도、재체내가완전강해、무세포독성화은축적독성등우점.
Objective To construct a kind of nanosilver-embedded collagen scaffold for peripheral nerve regeneration and to investigate its physical and chemical characters and biocompatibility.Methods The nanosilver particles at different concentrations of 1,2,3,4,5 and 6 mg/L were added into collagen respectively while pure water of the same amount was added in the control groups.Improved cryodesiccation was used to construct the nanosilver-embedded collagen scaffold with micro-channels.The nanosilver-embedded collagen scaffold suitable for the peripheral nerve was selected according to the diameters of micro-channels upon scanning electron microscopy and the tensile strength at the axial direction of the scaffold.The in vivo cytotoxicity of the selected scaffold was tested in 5 SD newborn rats,the in vivo degradation rate of the selected scaffold in 8 adult male SD rats and the in vivo cumulative toxicity of the nanosilver in 12 adult male SD rats.The best nanosilver-embedded collagen scaffold for peripheral nerve regeneration after injury was determined according to the comprehensive properties examined.Results The micro-channels of the nanosilver-embedded collagen scaffold were the most suitable for the peripheral nerve when the concentrations of nanosilver in the collagen were 1 or 2 mg/L.Tensile strength at the axial direction of the collagen scaffold embedded with 2 mg/L nanosilver was 0.22 MPa,better than the other groups.The collagen scaffold embedded with 2 mg/L nanosilver degraded in vivo gradually and completely after 4 months with no cytotoxicity or storage of toxicity.The contents of silver in the brain,spinal cord,liver and kidney were all lower than 1 ng/mL.Conclusion The collagen scaffold embedded with 2 mg/L nanosilver best suits the peripheral nerve regeneration after injury,because it has good tensile strength,can be fully degraded in vivo,and is free from cytotoxicity or storage of toxicity.
