CAJ | 학술논문

目的探讨肿瘤坏死因子-α基因启动子区域-308 (TNF-α-308)多态与骨关节炎(OA)遗传易感性的关系.方法随机选取2011年3月至2012年7月收治的200例膝关节OA患者(OA组)和同地区、无血缘关系的305名健康人群(对照组),均为汉族.采用TaqMan方法检测其TNF-α-308基因型分布及差异.OA组患者具有明确的OA症状、体征和影像学证据.两组研究对象的性别、年龄差异均无统计学意义(P＞0.05),具有可比性.结果 OA组和对照组的TNF-α-308A等位基因频率分别为16.0％和8.7％,A等位基因的OA发病风险是G等位基因的2.00倍[95％ CI(1.35,2.94),P=0.000].GA杂合子、AA纯合子和A等位基因携带者(GA或AA基因型)的OA风险分别是GG纯合子的2.03倍[95％ CI(1.27,3.27),P=0.003]、5.29倍[95％ CI(1.00,27.99,P=0.050)]和2.13倍[95％CI(1.35,3.35),P=0.001].将GG、GA和AA视为不同的等级,存在等位基因剂量-效应关系(P＜0.05).结论 TNF-α-308A可增加OA发病风险,该多态可作为中国OA高危人群的筛选指标.
목적 탐토종류배사인자-α기인계동자구역-308 (TNF-α-308)다태여골관절염(OA)유전역감성적관계.방법 수궤선취2011년3월지2012년7월수치적200례슬관절OA환자(OA조)화동지구、무혈연관계적305명건강인군(대조조),균위한족.채용TaqMan방법검측기TNF-α-308기인형분포급차이.OA조환자구유명학적OA증상、체정화영상학증거.량조연구대상적성별、년령차이균무통계학의의(P＞0.05),구유가비성.결과 OA조화대조조적TNF-α-308A등위기인빈솔분별위16.0％화8.7％,A등위기인적OA발병풍험시G등위기인적2.00배[95％ CI(1.35,2.94),P=0.000].GA잡합자、AA순합자화A등위기인휴대자(GA혹AA기인형)적OA풍험분별시GG순합자적2.03배[95％ CI(1.27,3.27),P=0.003]、5.29배[95％ CI(1.00,27.99,P=0.050)]화2.13배[95％CI(1.35,3.35),P=0.001].장GG、GA화AA시위불동적등급,존재등위기인제량-효응관계(P＜0.05).결론 TNF-α-308A가증가OA발병풍험,해다태가작위중국OA고위인군적사선지표.
Objective To investigate a possible association between TNF-α-308 polymorphism and susceptibility to osteoarthritis (OA) in a Chinese population.Methods We randomly recruited 200 OA patients who sought medical treatment in our department from March 2011 to July 2012 and 305 healthy controls in the same area.Their TNF-α-308 genotypes were determined by TaqMan assay and compared.All the subjects belonged to the Han ethnic group.The OA group had definite symptoms,signs and radiological evidence of the disease.The 2 groups were compatible in gender and age (P ＞ 0.05).Results The frequency of-308A allele was 0.160 in the OA group and 0.087 in the controls.Thus the-308A allele had a risk for OA 2.00-fold [95％ CI (1.35,2.94),P =0.000] higher than the-308G allele.When compared to GG homozygote,GA heterozygote had a risk for OA 2.03-fold higher [95％ CI (1.27,3.27),P =0.003],AA homozygote had a risk 5.29-fold higher [95％ CI (1.00,27.99),P =0.050] and carriers of the-308A allele (GA or AA genotypes) had a risk 2.13-fold higher [95％ CI (1.35,3.35),P =0.001].Taking GG,GA and AA as different grades,the Armitage's trend test showed a dose-effect relationship in the alleles.Conclusion Since the TNF-α-308A contributes to the elevated OA risk,this polymorphism can be used as a screening marker to identify individuals at risk for OA in the Chinese population.