CAJ | 학술논문

目的探讨不同剂量的X线对小鼠成骨细胞增殖与分化的影响. 方法将体外诱导培养的小鼠成骨细胞分为4组(n=3):对照组、0.1Gy组、0.5 Gy组、1.0 Gy组,4组细胞接种后24 h分别予以0、0.1、0.5、1.0 Gy剂量的X线照射,检测照射后l～3d细胞的增殖、细胞周期、凋亡情况,以及照射后7～14d碱性磷酸酶(ALP)、矿化结节形成与成骨相关基因的表达. 结果对照组、0.1 Gy组、0.5 Gy组、1.0Gy组成骨细胞的细胞周期和凋亡变化、细胞增殖比较差异均无统计学意义(P＞0.05).X线照射后培养7、10、14 d,0.5 Gy组和1.0Gy组细胞外ALP活性均较对照组高,差异均有统计学意义(P＜0.05).X线照射后14d,0.5 Gy组和1.0 Gy组矿化结节数目明显多于0.1 Gy组和对照组.1.0 Gy组骨钙素和核心结合因子α1 mRNA的表达较对照组增加,0.5Gy组和1.0 Gy组骨保护素mRNA/核因子-kB配体受体激活物mRNA比率均较对照组大,以上项目比较差异均有统计学意义(P＜0.05). 结论一定剂量范围(0.5～1.0 Gy)的X线照射在不影响成骨细胞增殖的情况下,可以促进成骨细胞的分化.
목적 탐토불동제량적X선대소서성골세포증식여분화적영향. 방법 장체외유도배양적소서성골세포분위4조(n=3):대조조、0.1Gy조、0.5 Gy조、1.0 Gy조,4조세포접충후24 h분별여이0、0.1、0.5、1.0 Gy제량적X선조사,검측조사후l～3d세포적증식、세포주기、조망정황,이급조사후7～14d감성린산매(ALP)、광화결절형성여성골상관기인적표체. 결과 대조조、0.1 Gy조、0.5 Gy조、1.0Gy조성골세포적세포주기화조망변화、세포증식비교차이균무통계학의의(P＞0.05).X선조사후배양7、10、14 d,0.5 Gy조화1.0Gy조세포외ALP활성균교대조조고,차이균유통계학의의(P＜0.05).X선조사후14d,0.5 Gy조화1.0 Gy조광화결절수목명현다우0.1 Gy조화대조조.1.0 Gy조골개소화핵심결합인자α1 mRNA적표체교대조조증가,0.5Gy조화1.0 Gy조골보호소mRNA/핵인자-kB배체수체격활물mRNA비솔균교대조조대,이상항목비교차이균유통계학의의(P＜0.05). 결론 일정제량범위(0.5～1.0 Gy)적X선조사재불영향성골세포증식적정황하,가이촉진성골세포적분화.
Objective To investigate the effects of different X-ray doses on the proliferation and differentiation of mouse osteoblasts in vitro.Methods The mouse osteoblastic-like cell line (MC3T3-E1)was cultured in vitro and divided into 4 groups (n =3).On the second day after seeding,the cells in the 4 groups (control group,0.1 Gy group,0.5 Gy group and 1.0 Gy group) were exposed to X-ray irradiation respectively at 4 different doses of 0,0.1,0.5,1.0 Gy.Cell proliferation,cell cycle and apoptosis were measured on day 1 to day 3 after irradiation; alkaline phosphatase (ALP) activity on days 7,10 and 14 and mineralization on day 14 were evaluated respectively.The mRNA expression of osteocalcin (OCN),core-binding factor α1 (Cbfα1),osteoprotegerin (OPG) and receptor activator of nuclear factor kappa B lig-and (RANKL) were measured by real time-PCR on day 14.Results Increasing doses of X-ray irradiation had no significant effects on cell proliferation,cell cycle or apoptosis compared with the control group(P ＞0.05).Importantly,X-ray irradiation of 0.5 Gy and 1.0 Gy increased ALP activities and mineralized nodules of osteoblasts,and markedly increased mRNA expressions of OPG/RANKL (P ＜ 0.05).Specially,mRNA expressions of OCN and Cbfα1 were also markedly increased after X-ray irradiation of 1.0 Gy (P ＜ 0.05).Conclusion X-ray irradiation at a certain rage of doses (from 0.5 to 1.0 Gy) can promote differentiation and mineralization of mouse osteoblasts but does not affect their proliferation and apoptosis.