CAJ | 학술논문

目的观察细胞因子和生长因子在人脐带Wharton胶间质干细胞(WMSCs)的表达,了解其生物学特性,为其临床应用提供实验依据. 方法取人足月妊娠新生儿脐带,分离MSCs,应用成纤维细胞集落形成实验验证其干细胞特性,流式细胞术鉴定其表面分子特性,蛋白芯片方法观察细胞因子和生长因子的表达. 结果所检测的79种因子除白介素(IL)-13表达痕量外,其他均表达于WMSCs,表达从多到少(OD值＞0.800)依次为IL-8、生长相关癌基因(GRO)、IL-6、IL-1β、IL-1α和GRO-α;它表达6种免疫抑制功能的因子和肿瘤生长抑制因子,这对减少MSCs用于异体移植时的免疫排斥及瘤变的可能性起一定作用,而其表达的金属蛋白酶抑制因子TIMP-1、TIMP-2将对其应用于体内移植时起到保护自身的作用;多种神经生长因子(脑源性神经生长因子、神经生长素-3、神经生长素-4、胶质细胞源性神经生长因子)的表达表明WMSCs易于向神经细胞诱导分化. 结论人脐带WMSCs易于获得,表达的细胞因子有利于其应用于异体移植,减少免疫排斥及恶变,并保护其自身在体内分化生长,是一个很有前景的新型种子细胞,可应用于再生医学及构建组织工程组织或器官.
목적 관찰세포인자화생장인자재인제대Wharton효간질간세포(WMSCs)적표체,료해기생물학특성,위기림상응용제공실험의거. 방법 취인족월임신신생인제대,분리MSCs,응용성섬유세포집락형성실험험증기간세포특성,류식세포술감정기표면분자특성,단백심편방법관찰세포인자화생장인자적표체. 결과 소검측적79충인자제백개소(IL)-13표체흔량외,기타균표체우WMSCs,표체종다도소(OD치＞0.800)의차위IL-8、생장상관암기인(GRO)、IL-6、IL-1β、IL-1α화GRO-α;타표체6충면역억제공능적인자화종류생장억제인자,저대감소MSCs용우이체이식시적면역배척급류변적가능성기일정작용,이기표체적금속단백매억제인자TIMP-1、TIMP-2장대기응용우체내이식시기도보호자신적작용;다충신경생장인자(뇌원성신경생장인자、신경생장소-3、신경생장소-4、효질세포원성신경생장인자)적표체표명WMSCs역우향신경세포유도분화. 결론 인제대WMSCs역우획득,표체적세포인자유리우기응용우이체이식,감소면역배척급악변,병보호기자신재체내분화생장,시일개흔유전경적신형충자세포,가응용우재생의학급구건조직공정조직혹기관.
Objective To characterize Wharton's jelly-derived mesenchymal stem cells from human umbilical cord (WMSCs) by observing their cytokine and growth factor expressions.Methods We isolated WMSCs from the umbilical cords from healthy full-term newborns.We verified the stemness by colony-forming unit-fibroblast (CFU-F) assay,detected the cell surface marker expression by flow cytometry,and observed the cytokine and growth factor expressions by protein chip assay.Results Human WMSCs were found to synthesize substantial levels of 78 of the 79 factors on the chip,with the only exception of IL-13.WMSCs were shown to produce significantly high levels [mean optical density (OD) ＞ 0.800] of the pro-inflammatory cytokines IL-lα,IL-1β,IL-6 and IL-8,as well as the chemokines growth related oncogene (GRO) and GRO-α.The expressed growth factors verified the osteogenesis and chondrogenesis differentiation potentials of WMSCs.In addition,the expression profile included 6 immune suppressive-related factors and tumor growth suppressors,which may reduce the chance of immune rejection and tumorigenesis of the allograft cells.Expressions of metalloproteases inhibitor,TIMP-1 and TIMP-2,could be important for the generation of functional tissue as they may prevent the breakdown of newly synthesized extracellular cell matrix (ECM) and protect the perivascular niche.Expressions of nerve growth factors (BDNF,NT-3,NT-4 and GDNF) indicate WMSCs have a potential to be induced and differentiated into nerve cells.Conclusions WMSCs are easy to obtain.The cytokines they express can reduce the chance of immune rejection and tumorigenesis and protect the implanted cells from metalloproteases digestion.So WMSCs may be a promising cellular candidate for use in regenerative medicine and fabrication of engineering tissue or organ.