CAJ | 학술논문

目的探讨银杏叶提取物(EGb761)对大鼠脊髓损伤后神经细胞凋亡、运动功能恢复的影响,以及其神经保护作用的可能机制. 方法取60只雌性SD大鼠,随机分为3组(n=20):假手术组、损伤对照组和EGb761治疗组.假手术组行椎板切除暴露脊髓,其余2组参照改良Allen法制造脊髓损伤模型.EGb761治疗组术后每天腹腔注射1次EGb761 100 mg/kg,假手术组和损伤对照组在同一时间给予等量生理盐水.于术后l、3、7、14d行后肢Basso Beattie Bresnahan(BBB)运动功能评分和足迹实验,并应用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法(TUNEL)染色和半胱氨酸蛋白酶-3染色观察神经细胞的凋亡情况. 结果假手术组大鼠术后后肢BBB运动功能评分和足迹实验正常.术后l、3、7d损伤对照组与EGb761治疗组大鼠后肢BBB运动功能评分和足迹实验无明显差异;而术后14d,EGb761治疗组大鼠BBB运动功能评分较损伤对照组高,步长较损伤对照组长,步宽较损伤对照组短,差异均有统计学意义(P＜0.05).术后1、3、7、14d,损伤对照组和EGb761治疗组TUNEL染色阳性细胞数、半胱氨酸蛋白酶-3染色阳性细胞数明显多于假手术组,术后3、7 d EGb761治疗组的TUNEL染色阳性细胞数及半胱氨酸蛋白酶-3染色阳性细胞数明显少于损伤对照组,差异均有统计学意义(P＜0.05). 结论 EGb761有明显的神经保护作用,其机制可能是通过下调半胱氨酸蛋白酶-3的表达,抑制伤部及邻近脊髓组织细胞凋亡来实现.
목적 탐토은행협제취물(EGb761)대대서척수손상후신경세포조망、운동공능회복적영향,이급기신경보호작용적가능궤제. 방법 취60지자성SD대서,수궤분위3조(n=20):가수술조、손상대조조화EGb761치료조.가수술조행추판절제폭로척수,기여2조삼조개량Allen법제조척수손상모형.EGb761치료조술후매천복강주사1차EGb761 100 mg/kg,가수술조화손상대조조재동일시간급여등량생리염수.우술후l、3、7、14d행후지Basso Beattie Bresnahan(BBB)운동공능평분화족적실험,병응용말단탈양핵감산전이매개도적dUTP결구말단표기측정법(TUNEL)염색화반광안산단백매-3염색관찰신경세포적조망정황. 결과 가수술조대서술후후지BBB운동공능평분화족적실험정상.술후l、3、7d손상대조조여EGb761치료조대서후지BBB운동공능평분화족적실험무명현차이;이술후14d,EGb761치료조대서BBB운동공능평분교손상대조조고,보장교손상대조조장,보관교손상대조조단,차이균유통계학의의(P＜0.05).술후1、3、7、14d,손상대조조화EGb761치료조TUNEL염색양성세포수、반광안산단백매-3염색양성세포수명현다우가수술조,술후3、7 d EGb761치료조적TUNEL염색양성세포수급반광안산단백매-3염색양성세포수명현소우손상대조조,차이균유통계학의의(P＜0.05). 결론 EGb761유명현적신경보호작용,기궤제가능시통과하조반광안산단백매-3적표체,억제상부급린근척수조직세포조망래실현.
Objective To observe the effect of ginkgo biloba extract (EGb761) on neuron apoptosis and motor functional recovery after spinal cord injury(SCI) and the possible underlying mechanisms.Methods Sixty SD female rats were randomly divided into 3 equal groups (n =20):sham-operation,Allen impact SCI and EGb761-treatmrnt.Egb761-treated group received intraperitoneal injection of 100 mg/kg EGb761 per day; the other 2 groups were given normal saline of the same amount at the same time.After Basso Beattie Bresnahan (BBB) assessment and footprint analysis of the hind limb were performed on days 1,3,7 and 14 post-injury in each group,the animals were sacrificed to extract the spinal cord for HE staining.Apoptosis of nerve cells was observed by immunohistochemistry using TdT-mediated dUTP nick end labeling (TUNEL) and 3,3 '-diaminobenzidine tetrahydrochloride(DAB).Results In the sham-operation group,no influence on the BBB scores or footprint analysis of the hind limb was observed.There was no significant difference in ethology assessments on days l,3 and 7 between the SCI and EGb761 groups,but on the 14th day after surgery,the BBB score was significantly higher,the step length was significantly longer,and the step width was significantly smaller in the EGb761 group than in the SCI group (P ＜ 0.05).On days 1,3,7 and 14,there were significantly more TUNEL-positive and Caspase-3 protein-positive cells in the SCI and EGb761 groups than in the sham-operation group (P ＜ 0.05) ; on days 3 and 7 after surgery,the TUNEL-positive and Caspase-3 protein-positive cells in the EGb761 group were significantly fewer than in the SCI group (P ＜0.05).Conclusions EGb761 has a positive protective effect on nervous injury after SCI.This protective effect may be realized by down-regulating the expression of Caspase-3 protein,inhibiting cellula nervosa (especially glial cells) apoptosis at injured and surrounding portions in the myeloid tissue.