中华超声影像学杂志
中華超聲影像學雜誌
중화초성영상학잡지
CHINESE JOURNAL OF ULTRASONOGRAPHY
2012年
12期
1078-1081
,共4页
郑剑%王平%尹庭辉%郑博文%程度%郑荣琴
鄭劍%王平%尹庭輝%鄭博文%程度%鄭榮琴
정검%왕평%윤정휘%정박문%정도%정영금
超声检查%微气泡%纳米技术
超聲檢查%微氣泡%納米技術
초성검사%미기포%납미기술
Ultrasonography%Microbubbles%Nanotechnology
目的 比较机械振荡法与声振法制备纳米级微泡超声造影剂(Nanobubbles,NBs)的效能.方法分别用机械振荡法和声振法制备NBs,比较两种方法制备NBs的粒径、粒径分布、浓度以及制备所耗时间等.结果 声振法与机械振荡法制备的NBs粒径分别为(373.88±18.43)nm、(360.74±14.39)nm,二者比较差异无统计学意义(P=0.523).声振法制备的NBs离心纯化前粒径分布较广,与机械振荡法制备的NBs粒径分布(分散系数,polidispersity值)比较差异有统计学意义(P＜0.001).机械振荡法制备的NBs浓度为(1.48±0.15)×1010,明显高于声振法制备的NBs浓度[(8.07±0.62)×108] (P＜0.001).声振法制备NBs较机械振荡法耗时长,两者比较差异有统计学意义(P＜0.001).结论 机械振荡法与声振法均能制备出NBs,但与声振法相比,机械振荡法制备的NBs粒径分布窄,浓度高,用时短,可更加快速有效地制备NBs,适合进行肿瘤超声造影成像方面的实验研究.
目的 比較機械振盪法與聲振法製備納米級微泡超聲造影劑(Nanobubbles,NBs)的效能.方法分彆用機械振盪法和聲振法製備NBs,比較兩種方法製備NBs的粒徑、粒徑分佈、濃度以及製備所耗時間等.結果 聲振法與機械振盪法製備的NBs粒徑分彆為(373.88±18.43)nm、(360.74±14.39)nm,二者比較差異無統計學意義(P=0.523).聲振法製備的NBs離心純化前粒徑分佈較廣,與機械振盪法製備的NBs粒徑分佈(分散繫數,polidispersity值)比較差異有統計學意義(P＜0.001).機械振盪法製備的NBs濃度為(1.48±0.15)×1010,明顯高于聲振法製備的NBs濃度[(8.07±0.62)×108] (P＜0.001).聲振法製備NBs較機械振盪法耗時長,兩者比較差異有統計學意義(P＜0.001).結論 機械振盪法與聲振法均能製備齣NBs,但與聲振法相比,機械振盪法製備的NBs粒徑分佈窄,濃度高,用時短,可更加快速有效地製備NBs,適閤進行腫瘤超聲造影成像方麵的實驗研究.
목적 비교궤계진탕법여성진법제비납미급미포초성조영제(Nanobubbles,NBs)적효능.방법분별용궤계진탕법화성진법제비NBs,비교량충방법제비NBs적립경、립경분포、농도이급제비소모시간등.결과 성진법여궤계진탕법제비적NBs립경분별위(373.88±18.43)nm、(360.74±14.39)nm,이자비교차이무통계학의의(P=0.523).성진법제비적NBs리심순화전립경분포교엄,여궤계진탕법제비적NBs립경분포(분산계수,polidispersity치)비교차이유통계학의의(P＜0.001).궤계진탕법제비적NBs농도위(1.48±0.15)×1010,명현고우성진법제비적NBs농도[(8.07±0.62)×108] (P＜0.001).성진법제비NBs교궤계진탕법모시장,량자비교차이유통계학의의(P＜0.001).결론 궤계진탕법여성진법균능제비출NBs,단여성진법상비,궤계진탕법제비적NBs립경분포착,농도고,용시단,가경가쾌속유효지제비NBs,괄합진행종류초성조영성상방면적실험연구.
Objective To compare the efficiency of oscillation with sonication in preparing nano-scale microbubbles (NBs).Methods Nano-scale microbubbles were prepared using oscillation and sonication respectively,and then compared the NBs' size,size distribution,concentrations and time-consumption of the two methods.Results The sizes of nanobubbles prepared by sonication and oscillation were (373.88 ±18.43)nm and (360.74 ± 14.39)nm,respectively.There was no significant difference in size between the two methods (P =0.523).The polidispersity was larger in sonication before centrifugation,there was significant difference between the two methods (P ＜0.001).The concentration of nanobubbles prepared by oscillation was (1.48 ± 0.15) × 1010,which was higher than that by oscillation [(8.07 ± 0.62) × 108],there was significant difference between the two methods (P ＜ 0.001).The consuming time was shorter in oscillation,the difference was significant when compared with sonication (P ＜0.001).Conclusions Both two methods can successfully prepare NBs.Compared with sonication,oscillation can effectively produce NBs with smaller polidispersity,higher concentration and shorter time-consumption.
