CAJ | 학술논문

目的应用超声造影评价超声介导的载血管内皮细胞生长因子抑制剂(vascular endothelial growth factor inhibitor,VEGFI)微泡对实验裸鼠结肠癌的治疗效果.方法 18只皮下种植结肠癌肿瘤的Balb/c裸鼠模型随机分为三组:A组(6只)为对照组,接受裸脂质微泡超声造影检查及假照；B组(6只),裸脂质微泡超声造影检查及超声辐照；C组(6只),载VEGFI脂质微泡及超声辐照.辐照前、辐照后1d、辐照后1周分别进行实时超声造影检查,存储图像进行声学定量脱机分析,获取峰值强度(PI)、局部血容量(RBV)、局部血流量(RBF)等造影参数并加以比较.结果辐照前三组PI、RBV、RBF差异无统计学意义(P＞0.05)；A组假照后1 dPI、RBV、RBF高于假照前,但差异无统计学意义(P＞0.05),1周后PI、RBV、RBF明显高于假照前,差异有统计学意义(P＜0.05)；B组辐照后1 dPI、RBF、RBV较辐照前减小,差异有统计学意义(P＜0.05),而1周后PI、RBF、RBV又上升,与辐照前比较差异无统计学意义(P＞0.05)；C组辐照后1d、辐照后1周PI、RBV、RBF均较辐照前减小,差异有统计学意义(P＜0.05).结论载VEGFI微泡联合超声辐照可以增强对实验裸鼠结肠癌的治疗效果.
목적 응용초성조영평개초성개도적재혈관내피세포생장인자억제제(vascular endothelial growth factor inhibitor,VEGFI)미포대실험라서결장암적치료효과.방법 18지피하충식결장암종류적Balb/c라서모형수궤분위삼조:A조(6지)위대조조,접수라지질미포초성조영검사급가조；B조(6지),라지질미포초성조영검사급초성복조；C조(6지),재VEGFI지질미포급초성복조.복조전、복조후1d、복조후1주분별진행실시초성조영검사,존저도상진행성학정량탈궤분석,획취봉치강도(PI)、국부혈용량(RBV)、국부혈류량(RBF)등조영삼수병가이비교.결과 복조전삼조PI、RBV、RBF차이무통계학의의(P＞0.05)；A조가조후1 dPI、RBV、RBF고우가조전,단차이무통계학의의(P＞0.05),1주후PI、RBV、RBF명현고우가조전,차이유통계학의의(P＜0.05)；B조복조후1 dPI、RBF、RBV교복조전감소,차이유통계학의의(P＜0.05),이1주후PI、RBF、RBV우상승,여복조전비교차이무통계학의의(P＞0.05)；C조복조후1d、복조후1주PI、RBV、RBF균교복조전감소,차이유통계학의의(P＜0.05).결론 재VEGFI미포연합초성복조가이증강대실험라서결장암적치료효과.
Objective To estimate the therapeutic efficacy of ultrasound destruction of vascular endothelial growth factor inhibitor (VEGFI)-carrying lipid microbubbles in colon cancer in nude mice.Methods Colon tumor xenografts cells were inoculated subcutaneously in eighteen Balb/c nude mice and tumor (5-20 mm) developed,all the mice were randomly divided into three groups:Group A (6 mice),as controls,underwent contrast-enhanced ultrasound (CEUS) examination and sham ultrasound radiation.Group B (6 mice) were given lipid microbubbles combined with ultrasound radiation.Group C (6mice)underwent VEGFI-carrying lipid microbubbles injection combined with ultrasound radiation.Contrastenhanced ultrasound (CEUS) examination was performed before and after ultrasound radiation.Enhanced peak intensity(PI),regional blood volume (RBV),regional blood flow (RBF) were recorded and compared.Results There was no significant difference in PI,RBV,RBF before radiation among the three groups (P＞0.05).PI,RBV,RBF in group A were higher 1 day after sham ultrasound radiation,the difference was not statistically significant (P ＞0.05),1 week later PI,RBV,RBF were higher than those before radiation,the difference was statistically significant (P ＜0.05).1 day after ultrasound radiation,PI,RBV,RBF in group B decreased,the difference was statistically significant (P ＜ 0.05),while after 1 week,PI,RBV,RBF increased respectively,comparing with those before ultrasound radiation,the difference was not statistically significant (P ＞0.05).The above indexes were significantly decreased in group C 1 day and 1 week after ultrasound radiation with statistically significant (P ＜ 0.05).Conclusions Ultrasound destruction of VEGFI-carrying lipid microbubbles can improve the therapeutic efficacy of colon cancer in nude mice.