CAJ | 학술논문

目的探讨超声辐射力对靶向微泡在体黏附性能的影响.方法建立小鼠提睾肌炎症模型,经尾静脉团注脂质微泡(MB)或靶向微泡(MBIcAM),同时进行超声辐射力(USRF)照射或假照5 min.实验随机分为MB+假照组和MB+ USRF组,MBIcAM+假照组和MBIcAM+ USRF组.荧光显微镜下观察微泡黏附情况并计数.结果 USRF组MBIcAM在微循环中的黏附数量比假照组显著增高,分别为(43.4±2.1)/视野,(14.8±1.8)/视野,两者之间具有显著性差异(P=0.000).USRF组MB在微血管中的黏附数量[(6.2±1.3)/视野)较假照组(4.6±0.9)/视野]有轻度增高,但两者之间差异无统计学意义(P=0.129).MBIcAM黏附数量在辐射力组及假照组均显著高于MB的黏附数量.结论超声辐射力可显著提高在体微血管内靶向微泡的黏附作用.
목적 탐토초성복사력대파향미포재체점부성능적영향.방법 건립소서제고기염증모형,경미정맥단주지질미포(MB)혹파향미포(MBIcAM),동시진행초성복사력(USRF)조사혹가조5 min.실험수궤분위MB+가조조화MB+ USRF조,MBIcAM+가조조화MBIcAM+ USRF조.형광현미경하관찰미포점부정황병계수.결과 USRF조MBIcAM재미순배중적점부수량비가조조현저증고,분별위(43.4±2.1)/시야,(14.8±1.8)/시야,량자지간구유현저성차이(P=0.000).USRF조MB재미혈관중적점부수량[(6.2±1.3)/시야)교가조조(4.6±0.9)/시야]유경도증고,단량자지간차이무통계학의의(P=0.129).MBIcAM점부수량재복사력조급가조조균현저고우MB적점부수량.결론 초성복사력가현저제고재체미혈관내파향미포적점부작용.
Objective To investigate the effects of ultrasound radiation force(USRF) on adhering of targeted microbubbles in vivo.Methods A rat model of cremaster muscle Inflammation was developed,and microbubble(MB) or targeted microbubble of intercellular adhesion molecule-1 (MBICAM) was injected through the caudal vein.Immediately after intravenous MB or MBICAM injection,the cremaster was insonated for 5 minutes and the control group was in sham ultrasound exposure.All mice were divided into four groups randomly:① MB + false USRF; ② MB + USRF; ③ MBICAM + false USRF; ④ MBICAM + USRF.Fluorescence microscope was performed in twenty mice to evaluate microbubbles adhesion and video recordings were made with a high-resolution camera.Results The adhesion number of MBICAM was significantly greater in USRF group than that of MBICAM in false USRF group [(43.4 ± 2.1)/view vs (14.8± 1.8)/view,P =0.000].The adhesion number of MB in USRF group was greater than in false USRF group [(6.2 ± 1.3)/view vs (4.6 ± 0.9)/view].But there is no statistical significance (P =0.129).The adhesion number of MBICAM in both USRF group and false USRF group was significantly greater than the adhesion number of MB.Conclusions The ultrasound radiation force can significantly improve the adhesion of targeted microbubbles in vivo.