CAJ | 학술논문

目的评价超声Flash技术联合声诺维(SonoVue)定向开放小鼠血脑屏障的可行性及安全性.方法 48只小鼠随机分为4组:空白组、微泡组、辐照组、超声辐照+微泡组,采用超声探头在Flash程序下联合静脉注射声诺维经颅骨定向辐照小鼠一侧基底节区,并利用伊文斯蓝示踪,荧光显微镜下观察脑组织中的伊文斯蓝荧光,定量分析脑组织伊文斯蓝浓度,并通过光镜和电镜观察脑组织大体细胞形态及超微结构改变.结果空白组、微泡组及辐照组肉眼均未见蓝染,荧光显微镜下未见明显红色荧光.超声辐照+微泡组基底节辐照区肉眼可见蓝染,镜下见大量红色荧光,脑组织内伊文斯蓝浓度明显高于其他组(P＜0.01).4组小鼠光镜及电镜观察均未见明显神经元损害及红细胞渗出,超声辐照+微泡组可见血管周围水肿及内皮细胞内吞饮小泡.结论超声Flash技术定向辐照联合静脉注射声诺维可无创、安全开放小鼠血脑屏障.
목적 평개초성Flash기술연합성낙유(SonoVue)정향개방소서혈뇌병장적가행성급안전성.방법 48지소서수궤분위4조:공백조、미포조、복조조、초성복조+미포조,채용초성탐두재Flash정서하연합정맥주사성낙유경로골정향복조소서일측기저절구,병이용이문사람시종,형광현미경하관찰뇌조직중적이문사람형광,정량분석뇌조직이문사람농도,병통과광경화전경관찰뇌조직대체세포형태급초미결구개변.결과 공백조、미포조급복조조육안균미견람염,형광현미경하미견명현홍색형광.초성복조+미포조기저절복조구육안가견람염,경하견대량홍색형광,뇌조직내이문사람농도명현고우기타조(P＜0.01).4조소서광경급전경관찰균미견명현신경원손해급홍세포삼출,초성복조+미포조가견혈관주위수종급내피세포내탄음소포.결론 초성Flash기술정향복조연합정맥주사성낙유가무창、안전개방소서혈뇌병장.
Objective To explore the safety and effectiveness of Flash technique of ultrasound associated with SonoVue to open the blood brain barrier(BBB) in mice.Methods Forty-eight mice were randomly divided into four groups:control group,microbubbles group,Flash group、Flash and microbubbles group.The right basal ganglia of the mice was sonicated by the flash procedures of ultrasound,through intact skull,after continuous intravenous injection of SonoVue and Evans blue (EB).The degree of BBB opening was evaluated quantitatively based on the extravasation of EB and qualitatively under the fluorescence microscope.The safety was inspected by observation of cell morphology under light microscopy and electron microscopy.Results Only in the Flash and microbubbles group,EB extravasation in the sonicated region and significantly red EB fluorescence under fluorescence microscope were shown,which couldn't be seen in other three groups.The EB concentration in the sonicated side in this group was also much higher than other groups (P ＜0.01).In all groups,there were no obvious neuronal damage and erythrocyte extravasation showed by HE staining and electron microscopy.Perivascular edema and vesicle within the cytoplasm of endothelial cells can be seen in the Flash and microbubbles group.Conclusions The BBB can be opened targetedly,safely and noninvasively by Flash procedure of ultrasound with the microbubbles in the mice.