CAJ | 학술논문

目的探讨超声辐照联合造影剂微泡介导EGFP转染类风湿性关节炎(RA)大鼠关节滑膜组织的可能性.方法 28只正常清洁级Wister大鼠,建立RA大鼠动物模型后,其中4只为空白对照组,其余24只再分为4个实验组,每组6只(12个膝关节).A组:单纯质粒组；B组:超声+质粒组；C组:微泡+质粒组；D组:超声+微泡+质粒组.各个实验组在不同的处理因素作用后3d处死大鼠,取膝关节滑膜组织,立即在荧光显微镜下观察EGFP的表达情况,同时取部分组织对EGFP进行RT-PCR定量检测分析.结果与空白对照组比较,实验各组RA大鼠关节滑膜组织中均有EGFP表达,但各组EGFP的表达存在差异.单纯质粒组、超声+质粒组、微泡+质粒组3组之间两两比较EGFP表达水平差异无统计学意义(P＞0.05)；单纯质粒组、超声+质粒组、微泡+质粒组3组与超声+微泡+质粒组之间EGFP表达水平比较差异有统计学意义(P＜0.01).结论超声击破微泡可以提高EGFP质粒对RA大鼠关节滑膜组织的转染效率.
목적 탐토초성복조연합조영제미포개도EGFP전염류풍습성관절염(RA)대서관절활막조직적가능성.방법 28지정상청길급Wister대서,건립RA대서동물모형후,기중4지위공백대조조,기여24지재분위4개실험조,매조6지(12개슬관절).A조:단순질립조；B조:초성+질립조；C조:미포+질립조；D조:초성+미포+질립조.각개실험조재불동적처리인소작용후3d처사대서,취슬관절활막조직,립즉재형광현미경하관찰EGFP적표체정황,동시취부분조직대EGFP진행RT-PCR정량검측분석.결과 여공백대조조비교,실험각조RA대서관절활막조직중균유EGFP표체,단각조EGFP적표체존재차이.단순질립조、초성+질립조、미포+질립조3조지간량량비교EGFP표체수평차이무통계학의의(P＞0.05)；단순질립조、초성+질립조、미포+질립조3조여초성+미포+질립조지간EGFP표체수평비교차이유통계학의의(P＜0.01).결론 초성격파미포가이제고EGFP질립대RA대서관절활막조직적전염효솔.
Objective To explore the feasibility of enhanced green fluorescent protein (EGFP) transfection into the joint synovial tissues of rheumatoid arthritis (RA) rats by ultrasound-mediated microbubble destruction.Methods Twenty-eight normal rats were established the RA rat model,four rats were control group,twenty-four rats were categorized into four experimental groups:EGFP,ultrasound +EGFP,microbubbles + EGFP,and ultrasound + microbubbles + EGFP.The last group was irradiated with ultrasound for 10 min after the mixture consisting of 300 μl Sono Vue and 10 μg EGFP was injected into the joint cavity.The rats were sacrificed after 3 days and the joint synovial tissues were collected for EGFP observation under fluorescence microscopy and quantitative analysis by real-time polymerase chain reaction (RT-PCR).Results Comparing with control group,EGFP expression was observed in the rat joint synovial tissues from all groups.However,a strong EGFP expression was observed in the ultrasound + microbubbles +EGFP group.EGFP expression had no statistically significant difference (the P values were 0.89,0.93,and 0.82,respectively,P ＞ 0.05) in the EGFP,ultrasound + EGFP and microbubbles + EGFP groups.However,EGFP expression in the EGFP,ultrasound + EGFP,microbubbles + EGFP groups significantly differed (all P values were ＜0.01) from that in the ultrasound + microbubbles + EGFP group.Conclusions Ultrasound-mediated microbubble destruction can improve EGFP transfection efficiency into the joint synovial tissues of RA rats.