CAJ | 학술논문

目的探讨血管回声追踪及频谱多普勒技术评价高脂血症及动脉粥样硬化大鼠不同阶段腹主动脉弹性功能变化的价值及差异.方法应用血管回声追踪及频谱多普勒技术分别对高胆固醇饮食SD大鼠(T1组,10只,高胆固醇喂养4周;T2组,10只,高胆固醇喂养12周)及正常饮食SD大鼠(C1组,10只;C2组,10只)腹主动脉的硬化系数(β)、可扩张度(DC)、顺应性(AC)、单点脉搏波传导速度(PWVβ)、阻力指数(RI)、收缩期峰值流速(PSV)、舒张期末流速(EDV)及EDV/PSV进行评估,各组间参数的比较及血压、血脂的组间比较采用ANOVE单因素分析法.相关性研究采用Pearson相关性分析法.各组均行病理检查以评估主动脉粥样硬化的程度.结果 T2的PSV明显高于T1组(P＜0.05).T1和T2组大鼠的β、PWVβ显著高于C1及C2组(P均＜0.05),DC及AC则显著低于C1及C2组(P均＜0.05),T2组的PWVβ明显高于T1组(P=0.001).相关性分析显示,RI仅与收缩压呈正相关(P＜0.05).收缩压、舒张压、β、PWVβ、AC、DC、甘油三酯、总胆固醇间存在明显的相关性(P＜0.05).其中AC、DC与β、PWVβ呈负相关,DC与甘油三酯呈负相关.结论血管回声追踪技术较频谱多普勒技术敏感,能早期检测大鼠腹主动脉弹性功能变化,其参数均与血脂参数相关.PSV的升高是诊断大鼠腹主动脉粥样硬化的一个较好的血流动力学指标,但频谱多普勒在检测早期血管弹性方面有局限性.
목적 탐토혈관회성추종급빈보다보륵기술평개고지혈증급동맥죽양경화대서불동계단복주동맥탄성공능변화적개치급차이.방법 응용혈관회성추종급빈보다보륵기술분별대고담고순음식SD대서(T1조,10지,고담고순위양4주;T2조,10지,고담고순위양12주)급정상음식SD대서(C1조,10지;C2조,10지)복주동맥적경화계수(β)、가확장도(DC)、순응성(AC)、단점맥박파전도속도(PWVβ)、조력지수(RI)、수축기봉치류속(PSV)、서장기말류속(EDV)급EDV/PSV진행평고,각조간삼수적비교급혈압、혈지적조간비교채용ANOVE단인소분석법.상관성연구채용Pearson상관성분석법.각조균행병리검사이평고주동맥죽양경화적정도.결과 T2적PSV명현고우T1조(P＜0.05).T1화T2조대서적β、PWVβ현저고우C1급C2조(P균＜0.05),DC급AC칙현저저우C1급C2조(P균＜0.05),T2조적PWVβ명현고우T1조(P=0.001).상관성분석현시,RI부여수축압정정상관(P＜0.05).수축압、서장압、β、PWVβ、AC、DC、감유삼지、총담고순간존재명현적상관성(P＜0.05).기중AC、DC여β、PWVβ정부상관,DC여감유삼지정부상관.결론 혈관회성추종기술교빈보다보륵기술민감,능조기검측대서복주동맥탄성공능변화,기삼수균여혈지삼수상관.PSV적승고시진단대서복주동맥죽양경화적일개교호적혈류동역학지표,단빈보다보륵재검측조기혈관탄성방면유국한성.
Objective To ascertain the utility and difference of sonography with echo-tracking (ET) technique and pulse-Doppler to assess vascular stiffness in rats with hypercholesterolemia and atherosclerosis.Methods Sonography associated with ET technique and pulse-Doppler were used to measure stiffness parameter (β),arterial compliance (AC),distensibility coefficient (DC),one-point pulse wave velocity (PWVβ),resistence index(RI),peak systolic velocity(PSV),end-diastolic velocity(EDV) and EDV/PSV of the aorta in cholesterol-fed SD rats (group T1,n =10,for 4 weeks;group T2,n =10,for 12 weeks) and normal control rats(group C1,n =10;group C2,n =10).All parameters and blood biochemical markers[total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-CH) and highdensity lipoprotein cholesterol (HDL-CH)] among groups were analyzed with ANOVE factor analysis.Correlation was analyzed with Pearson analysis.Light microscopic evaluation were used to demonstrate atherosclerotic changes in the aorta.Results The PWVβ value and PSV of the aorta between group T1 and T2 were significantly different (P =0.001,P ＜0.05).The β,PWVβ values of the aorta in group T1 and T2 were significantly higher than those of group C1 and C2 (P ＜0.05).AC and DC values of the aorta in group T1 and T2 were significantly lower than those of group C1 and C2 (P ＜0.05).Correlation analysis showsed that RI was positively correlated with systolic pressure(P ＜0.05).All parameters had correlated with each other among β,PWVβ,AC,DC,TG,TC,systolic pressure and diastolic pressure.DC and AC were negatively correlated with β and PWVβ,also DC was negatively correlated with TG.Light microscopy confirmed morphologic typical changes of aortic atherosclerosis in group T1 and T2.Conclusions Sonography with the ET method compared with pulse-Doppler is much more sensitive and it can be used to evaluate tissue elastic changes in arterial walls associated with atherosclerosis and hypercholesterolemia.PSV can reflect atherosclerosis of rat's abdominal aorta well,but pulse-Doppler is limited in the diagnosis of earlier atherosclerosis period.