CAJ | 학술논문

目的探讨超声靶向微泡破坏(UTMD)增强万古霉素活性治疗生物膜感染的效果.方法应用超声靶向微泡分组处理RP62A生物膜,结晶紫染色后OD570半定量检测,肉眼、光镜及激光共聚焦扫描显微镜观察生物膜的形态与细菌存活情况,菌落形成单位计数进一步对其内存活细菌进行定量检测.结果各超声或超声+微泡组的生物膜可见许多微孔,其中超声+微泡组和万古霉素+超声组生物膜微孔显著较大,OD570半定量检测表明超声+微泡组和万古霉素+超声+微泡组生物膜A570值最低(P＜0.01).共聚焦激光扫描显微镜观察证实了生物膜微孔的存在,万古霉素+超声+微泡组较其他各组有更多的死菌(P＜0.01).菌落形成单位计数进一步证实了上述结果.结论 UTMD可有效提高抗生素活性,杀灭表皮葡萄球菌生物膜内细菌.
목적 탐토초성파향미포파배(UTMD)증강만고매소활성치료생물막감염적효과.방법 응용초성파향미포분조처리RP62A생물막,결정자염색후OD570반정량검측,육안、광경급격광공취초소묘현미경관찰생물막적형태여세균존활정황,균락형성단위계수진일보대기내존활세균진행정량검측.결과 각초성혹초성+미포조적생물막가견허다미공,기중초성+미포조화만고매소+초성조생물막미공현저교대,OD570반정량검측표명초성+미포조화만고매소+초성+미포조생물막A570치최저(P＜0.01).공취초격광소묘현미경관찰증실료생물막미공적존재,만고매소+초성+미포조교기타각조유경다적사균(P＜0.01).균락형성단위계수진일보증실료상술결과.결론 UTMD가유효제고항생소활성,살멸표피포도구균생물막내세균.
Objective To investigated whether ultrasound (US)-targeted microbubble (MB) destruction (UTMD) can enhance antibiotic activity.Methods Twenty-four hours biofilms of S.epidermidis RP62A were treated with different parameters in 6 groups.After the treatments,the absorbance values (A570) of biofilms stained with the crystal violet were measured to assess the density of biofilms.The biofilms were observed under macroscopy and light microscopy,and the morphology of biofilms were also examined and analyzed by confocal laser-scanning microscopy (CLSM).The viable log10CFU among biofilms in different groups were compared.Results Many micropores were found in the biofiims treated with US and/or MB group.Even bigger micropores could be found in the biofilms of US + MB group and vancomycin+ US + MB group,with even smaller A570 values (P ＜ 0.01).The CLSM examination confirmed the existence of the micropores and showed that the highest proportions of dead cells were found in the vancomycin+ MB + US group,and the results were confirmed further by the experiments of viable log10CFU counts of biofilms.Conclusions UTMD might have a great potential in increasing antibiotic activity and reducing viable bacteria in biofilm.