CAJ | 학술논문

目的研制一种包裹金纳米棒的PLGA液态氟碳纳米粒;观察其在体外实验中的光声成像效果,以及产生液气相变的条件和相变过程中光声信号的变化情况.方法采用双乳化法制备包裹金纳米棒(gold nanorod,GNR)及液体氟碳的PLGA纳米粒(gold nano particle,GNP).实验分为3组:包裹金纳米棒及液态氟碳的PLGA纳米粒(GNP组)、金纳米棒(GNR组)及未包裹金纳米棒的PLGA液态氟碳纳米粒(PLGA组).利用脉冲激光触发3组样品,光声成像仪器记录光声信号变化;光学显微镜观察微球液气相变情况.结果成功制备包裹金纳米棒的PLGA液态氟碳纳米粒,其平均粒径为504.9 nm;激光触发后,光学显微镜下观察到GNP组发生液气相变,光声成像仪记录到GNP组和GNR组明显的光声信号,且信号强度随金纳米棒含量增加而增加;而对照组PLGA组未观察到光声信号;GNP组时间-光声信号强度曲线呈现先下降后稳定的趋势,而GNR组光声信号稳定未见明显变化.结论包裹金纳米棒的PLGA液态氟碳纳米粒在激光的触发下能发生液气相变,且产生较强的光声信号,从而使其能成为良好的光声成像对比剂,为今后光声成像的体内研究以及光热治疗打下了实验基础.
목적 연제일충포과금납미봉적PLGA액태불탄납미립;관찰기재체외실험중적광성성상효과,이급산생액기상변적조건화상변과정중광성신호적변화정황.방법 채용쌍유화법제비포과금납미봉(gold nanorod,GNR)급액체불탄적PLGA납미립(gold nano particle,GNP).실험분위3조:포과금납미봉급액태불탄적PLGA납미립(GNP조)、금납미봉(GNR조)급미포과금납미봉적PLGA액태불탄납미립(PLGA조).이용맥충격광촉발3조양품,광성성상의기기록광성신호변화;광학현미경관찰미구액기상변정황.결과 성공제비포과금납미봉적PLGA액태불탄납미립,기평균립경위504.9 nm;격광촉발후,광학현미경하관찰도GNP조발생액기상변,광성성상의기록도GNP조화GNR조명현적광성신호,차신호강도수금납미봉함량증가이증가;이대조조PLGA조미관찰도광성신호;GNP조시간-광성신호강도곡선정현선하강후은정적추세,이GNR조광성신호은정미견명현변화.결론 포과금납미봉적PLGA액태불탄납미립재격광적촉발하능발생액기상변,차산생교강적광성신호,종이사기능성위량호적광성성상대비제,위금후광성성상적체내연구이급광열치료타하료실험기출.
Objective To synthesize a photoacoustic contrast agent loaded with gold nanorod and liquid perflurocarbon which was used to observe its photoacoustic imaging in vitro,and then investigate the variation of the photoacoustic signal during and after the vaporization.Methods The PLGA nanoparticle (GNP)loaded with gold nanorod(GNR) and liquid perflurocarbon was synthesized via double emulsion technology.The experiments were divided into three groups:1)GNP group:PLGA nanoparticle loaded with gold nanorod and liquid perflurocarbon; 2) GNR group:gold nanorod ; 3) control group:PLGA nanoparticle unloaded with gold nanorod.A pulsed laser system was used to trigger the three samples.The photoacoustic image and time-signal intensity kinetics were acquired by the photoacoustic microscope.The optical microscope was used to observe the image after the vaporization.Results The gold nanoparticle was successfully prepared,the average size of the gold nanoparticle was 504.9 nm.after triggered by the pulsed laser system,the vaporized image of the GNP group could be observed by the optical microscope.The photoacoustic signal was detected in both GNP and GNR groups,and the signal intensity increased along with the concentration of the GNR.However,the photoacoustic signal of PLGA group was not detected.The photoacoustic signal kinetics of GNP group showed a descending trend from high signal amplitude to steadystate.The GNR group photoacoustic signal kinetics only showed a steady-state signal amplitude.Conclusions The PLGA nanoparticle loaded with gold nanorod and liquid perflurocarbon emerged a phase transient which produces a strong photoacoustic signal after triggered by pulsed laser system.This kind of phenomenon leads the nanoparticle to be a better photoacoustic contrast agent and lays a foundation for in vivo research and photothermal therapy in vivo.