CAJ | 학술논문

目的运用超声造影评价重组人内皮抑制素(恩度)对兔腹主动脉粥样斑块内新生血管的影响.方法对经球囊拉伤腹主动脉后予高脂喂养12周的26只造模成功雄性新西兰大白兔进行常规超声及超声造影检查,检查完毕随机处死8只兔(A组,病理对照组)取腹主动脉斑块标本进行病理检查,剩余被随机分为2组:B组(9只,对照组),再以普通饲料喂养4周;C组(9只,治疗组),再以普通饲料喂养加恩度治疗4周.16周再行常规超声及超声造影检查,检查完毕后立即处死所有模型,作病理学检查.结果 12周时各组斑块平均厚度、面积狭窄率、达峰时间(TTP)、峰值强度(PI)、增强强度(EI)之间比较差异均无统计学意义(P＞0.05).经过4周恩度治疗,C组斑块EI、微血管密度(MVD)及易损斑块比例与B组同期及A组比较均有明显下降,差异有统计学意义(P＜0.05),余各指标与A组比较差异均无统计学意义(P＞0.05);而B组16周时各指标与A组比较差异均无统计学意义(P＞0.05).结论恩度短期治疗能抑制斑块内新生血管的生长,有助于斑块的稳定;超声造影能有效地评价恩度对斑块内新生血管的抑制作用.
목적 운용초성조영평개중조인내피억제소(은도)대토복주동맥죽양반괴내신생혈관적영향.방법 대경구낭랍상복주동맥후여고지위양12주적26지조모성공웅성신서란대백토진행상규초성급초성조영검사,검사완필수궤처사8지토(A조,병리대조조)취복주동맥반괴표본진행병리검사,잉여피수궤분위2조:B조(9지,대조조),재이보통사료위양4주;C조(9지,치료조),재이보통사료위양가은도치료4주.16주재행상규초성급초성조영검사,검사완필후립즉처사소유모형,작병이학검사.결과 12주시각조반괴평균후도、면적협착솔、체봉시간(TTP)、봉치강도(PI)、증강강도(EI)지간비교차이균무통계학의의(P＞0.05).경과4주은도치료,C조반괴EI、미혈관밀도(MVD)급역손반괴비례여B조동기급A조비교균유명현하강,차이유통계학의의(P＜0.05),여각지표여A조비교차이균무통계학의의(P＞0.05);이B조16주시각지표여A조비교차이균무통계학의의(P＞0.05).결론 은도단기치료능억제반괴내신생혈관적생장,유조우반괴적은정;초성조영능유효지평개은도대반괴내신생혈관적억제작용.
Objective To evaluate the effect of endostar on neovascularization within rabbits of abdominal aortic atherosclerotic plaques using contrast-enhanced ultrasonography (CEUS).Methods Twenty six male New Zealand rabbits,damaged abdominal aorta by expanded balloon,high-fat feeding for consecutive twelve weeks,made plaque model successfully,were detected using conventional ultrasound examination and CEUS.Group A (eight rabbits) as pathology control group was killed immediately randomly after examination,then every abdominal aortic plaque were taken out for pathological examination and immunohistochemical detection.Survivals were divided into two groups randomly:Group B (nine as control group) fed common diet for another four weeks; Group C (nine as treatment group) injected endostar besides common diet.All survivals were detected using conventional ultrasound examination and CEUS once more in sixteen weeks,then killed immediately and all plaques were made for pathological examination.Results The value of maximum thickness,luminal stenosis rate,time to peak(TTP),peak intensity(PI) and enhanced intensity (EI) compared among three groups had no significant difference(P ＞0.05).After four weeks treatment,EI,microvessel density (MVD) and the rate of vulnerable plaques in group C were significantly less than group A and the same time group B,and the value of contrast between the two groups was significantly different (P ＜ 0.05).Others value compared with group A had no significant difference P ＞ 0.05).However,every value in group B compared with group A had no significant difference (P ＞ 0.05).Conclusions EI could accurately evaluate the effect of endostar on neovascularization within rabbits of abdominal aortic atherosclerotic plaques,at the same time showed endostar could suppress neovascularization of atherosclerotic plaques,which contributed to them become stable.