CAJ | 학술논문

目的探索携氧载紫杉醇脂质微泡体外巨噬细胞化的可行性及相关影响因素.方法采用机械振动法合成携氧载紫杉醇脂质微泡,与体外培养的小鼠腹腔巨噬细胞共孵育,普通光镜观察不同孵育时间下巨噬细胞对不同粒径、不同浓度的脂质微泡的吞噬率.结果携氧载紫杉醇脂质微泡体可成功被巨噬细胞吞噬,其吞噬效率与微泡粒径、浓度及二者共孵育时间密切相关.随着微泡粒径增大,巨噬细胞对微泡的吞噬率下降;巨噬细胞与微泡的比例1∶10,共孵育30 min吞噬率最高,达到(18.9±0.69)％.结论携氧载紫杉醇脂质微泡可成功体外巨噬细胞化,微泡粒径、浓度及二者共孵育时间均是影响微泡巨噬细胞化效率的重要因素.
목적 탐색휴양재자삼순지질미포체외거서세포화적가행성급상관영향인소.방법 채용궤계진동법합성휴양재자삼순지질미포,여체외배양적소서복강거서세포공부육,보통광경관찰불동부육시간하거서세포대불동립경、불동농도적지질미포적탄서솔.결과 휴양재자삼순지질미포체가성공피거서세포탄서,기탄서효솔여미포립경、농도급이자공부육시간밀절상관.수착미포립경증대,거서세포대미포적탄서솔하강;거서세포여미포적비례1∶10,공부육30 min탄서솔최고,체도(18.9±0.69)％.결론 휴양재자삼순지질미포가성공체외거서세포화,미포립경、농도급이자공부육시간균시영향미포거서세포화효솔적중요인소.
Objective To investigate the feasibility and parameters for macrophage uptake oxygen and paclitaxel loaded lipid microbubbles (OPLMBs) in vitro.Methods OPLMBs were synthesized by mechanical vibration method and incubated with macrophages in different conditions in vitro.The ratio of macrophages uptake OPLMBs was observed by bright field microscope.Results The ratio of macrophages uptake OPLMBs was closely related to the size,concentration as well as the incubation time of OPLMBs with the macrophage.The ratio of macrophages uptake OPLMBs had a reverse relationship with the size of microbubbles.The highest rate of macrophages uptake OPLMBs was (18.9 ± 0.69)％ when the ratio of macrophages to microbubbles was 1 to 10,the time for macrophages co-incubation with microbubbles was 30 min,which was the suitable condition for macrophages uptake OPLMBs.Conclusions OPLMBs can be phagocytized successfully by macrophages in vitro.The size,concentration as well as the incubation time of OPLMBs with the macrophages are important parameters contributed to the efficiency of macrophage phagocytosis.