中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2012年
11期
818-822
,共5页
魏莉娜%方丛%黄睿%李俐琳%张敏芳%梁晓燕
魏莉娜%方叢%黃睿%李俐琳%張敏芳%樑曉燕
위리나%방총%황예%리리림%장민방%량효연
生长分化因子9%骨形态发生蛋白质15%多囊卵巢综合征%卵母细胞
生長分化因子9%骨形態髮生蛋白質15%多囊卵巢綜閤徵%卵母細胞
생장분화인자9%골형태발생단백질15%다낭란소종합정%란모세포
Growth differentiation factor 9%Bone morphogenctic protein 15%Polycystic ovary syndrome%Oocytes
目的 探讨卵源性因子——生长分化因子9(GDF9)和骨形态发生蛋白15(BMP15)在多囊卵巢综合征(PCOS)患者及因男方因素不孕的卵巢功能正常妇女刺激周期卵母细胞不同成熟阶段的表达变化及意义.方法 收集12例PCOS患者(PCOS组)的25个卵母细胞[9个胚泡(CV)期,9个MⅠ期和7个M Ⅱ期]和56例卵巢功能正常妇女(对照组)的82个卵母细胞(29个GV期,26个MⅠ期和27个MⅡ期),采用巢式实时定量PCR技术,检测两组妇女不同成熟阶段的卵母细胞中GDF9和BMP15 mRNA的表达水平.结果(1)GDF9 mRNA的表达水平:PCOS组GV、MⅠ、MⅡ期卵母细胞GDF9 mRNA的表达水平分别为44.8(4.2 ～529.0)、27.6(9.8～172.7)和49.0(0.2～65.9),对照组GV、MⅠ、MⅡ期卵母细胞GDF9 mRNA的表达水平分别为149.9(55.4 ～ 387.9)、29.9(2.5 ～205.8)和657.8(149.4 ～1376.2),两组妇女MⅡ期卵母细胞GDF9 mRNA的表达水平分别比较,差异有统计学意义(P＜0.01),但两组妇女GV、MⅠ期卵母细胞GDF9 mRNA的表达水平比较,差异无统计学意义(P＞0.05);PCOS组不同成熟阶段卵母细胞GDF9 mRNA的表达水平比较,差异无统计学意义(P＞0.05),对照组不同成熟阶段卵母细胞GDF9 mRNA的表达水平分别比较,差异均有统计学意义(P ＜0.05、P＜0.01).(2)BMP15 mRNA的表达水平:PCOS组GV、MⅠ、MⅡ期卵母细胞BMP15 mRNA的表达水平分别为0.1(0.1 ～22.0)、3.2(0.6～55.0)和6.4(3.2～8.5),对照组GV、MⅠ、MⅡ期卵母细胞BMP15 mRNA的表达水平分别为41.6(6.5 ～96.1)、4.0(2.0～10.4)和49.7(2.3 ～139.5),两组妇女GV期卵母细胞BMP15 mRNA的表达比较,差异有统计学意义(P＜0.01),但两组MⅠ、MⅡ期卵母细胞BMP15 mRNA的表达水平比较,差异均无统计学意义(P＞0.05);PCOS组不同成熟阶段卵母细胞BMP15 mRNA的表达水平比较,差异无统计学意义(P＞0.05),对照组不同成熟阶段卵母细胞BMP15 mRNA的表达水平比较,GV期高于MⅠ期(P＜0.05),MⅠ期低于MⅡ期(P＜0.01).结论在刺激周期中,PCOS患者成熟卵母细胞中卵源性因子表达水平降低,可能影响细胞质成熟,从而导致PCOS患者卵母细胞质量下降和发育潜能受损.
目的 探討卵源性因子——生長分化因子9(GDF9)和骨形態髮生蛋白15(BMP15)在多囊卵巢綜閤徵(PCOS)患者及因男方因素不孕的卵巢功能正常婦女刺激週期卵母細胞不同成熟階段的錶達變化及意義.方法 收集12例PCOS患者(PCOS組)的25箇卵母細胞[9箇胚泡(CV)期,9箇MⅠ期和7箇M Ⅱ期]和56例卵巢功能正常婦女(對照組)的82箇卵母細胞(29箇GV期,26箇MⅠ期和27箇MⅡ期),採用巢式實時定量PCR技術,檢測兩組婦女不同成熟階段的卵母細胞中GDF9和BMP15 mRNA的錶達水平.結果(1)GDF9 mRNA的錶達水平:PCOS組GV、MⅠ、MⅡ期卵母細胞GDF9 mRNA的錶達水平分彆為44.8(4.2 ～529.0)、27.6(9.8～172.7)和49.0(0.2～65.9),對照組GV、MⅠ、MⅡ期卵母細胞GDF9 mRNA的錶達水平分彆為149.9(55.4 ～ 387.9)、29.9(2.5 ～205.8)和657.8(149.4 ～1376.2),兩組婦女MⅡ期卵母細胞GDF9 mRNA的錶達水平分彆比較,差異有統計學意義(P＜0.01),但兩組婦女GV、MⅠ期卵母細胞GDF9 mRNA的錶達水平比較,差異無統計學意義(P＞0.05);PCOS組不同成熟階段卵母細胞GDF9 mRNA的錶達水平比較,差異無統計學意義(P＞0.05),對照組不同成熟階段卵母細胞GDF9 mRNA的錶達水平分彆比較,差異均有統計學意義(P ＜0.05、P＜0.01).(2)BMP15 mRNA的錶達水平:PCOS組GV、MⅠ、MⅡ期卵母細胞BMP15 mRNA的錶達水平分彆為0.1(0.1 ～22.0)、3.2(0.6～55.0)和6.4(3.2～8.5),對照組GV、MⅠ、MⅡ期卵母細胞BMP15 mRNA的錶達水平分彆為41.6(6.5 ～96.1)、4.0(2.0～10.4)和49.7(2.3 ～139.5),兩組婦女GV期卵母細胞BMP15 mRNA的錶達比較,差異有統計學意義(P＜0.01),但兩組MⅠ、MⅡ期卵母細胞BMP15 mRNA的錶達水平比較,差異均無統計學意義(P＞0.05);PCOS組不同成熟階段卵母細胞BMP15 mRNA的錶達水平比較,差異無統計學意義(P＞0.05),對照組不同成熟階段卵母細胞BMP15 mRNA的錶達水平比較,GV期高于MⅠ期(P＜0.05),MⅠ期低于MⅡ期(P＜0.01).結論在刺激週期中,PCOS患者成熟卵母細胞中卵源性因子錶達水平降低,可能影響細胞質成熟,從而導緻PCOS患者卵母細胞質量下降和髮育潛能受損.
목적 탐토란원성인자——생장분화인자9(GDF9)화골형태발생단백15(BMP15)재다낭란소종합정(PCOS)환자급인남방인소불잉적란소공능정상부녀자격주기란모세포불동성숙계단적표체변화급의의.방법 수집12례PCOS환자(PCOS조)적25개란모세포[9개배포(CV)기,9개MⅠ기화7개M Ⅱ기]화56례란소공능정상부녀(대조조)적82개란모세포(29개GV기,26개MⅠ기화27개MⅡ기),채용소식실시정량PCR기술,검측량조부녀불동성숙계단적란모세포중GDF9화BMP15 mRNA적표체수평.결과(1)GDF9 mRNA적표체수평:PCOS조GV、MⅠ、MⅡ기란모세포GDF9 mRNA적표체수평분별위44.8(4.2 ～529.0)、27.6(9.8～172.7)화49.0(0.2～65.9),대조조GV、MⅠ、MⅡ기란모세포GDF9 mRNA적표체수평분별위149.9(55.4 ～ 387.9)、29.9(2.5 ～205.8)화657.8(149.4 ～1376.2),량조부녀MⅡ기란모세포GDF9 mRNA적표체수평분별비교,차이유통계학의의(P＜0.01),단량조부녀GV、MⅠ기란모세포GDF9 mRNA적표체수평비교,차이무통계학의의(P＞0.05);PCOS조불동성숙계단란모세포GDF9 mRNA적표체수평비교,차이무통계학의의(P＞0.05),대조조불동성숙계단란모세포GDF9 mRNA적표체수평분별비교,차이균유통계학의의(P ＜0.05、P＜0.01).(2)BMP15 mRNA적표체수평:PCOS조GV、MⅠ、MⅡ기란모세포BMP15 mRNA적표체수평분별위0.1(0.1 ～22.0)、3.2(0.6～55.0)화6.4(3.2～8.5),대조조GV、MⅠ、MⅡ기란모세포BMP15 mRNA적표체수평분별위41.6(6.5 ～96.1)、4.0(2.0～10.4)화49.7(2.3 ～139.5),량조부녀GV기란모세포BMP15 mRNA적표체비교,차이유통계학의의(P＜0.01),단량조MⅠ、MⅡ기란모세포BMP15 mRNA적표체수평비교,차이균무통계학의의(P＞0.05);PCOS조불동성숙계단란모세포BMP15 mRNA적표체수평비교,차이무통계학의의(P＞0.05),대조조불동성숙계단란모세포BMP15 mRNA적표체수평비교,GV기고우MⅠ기(P＜0.05),MⅠ기저우MⅡ기(P＜0.01).결론재자격주기중,PCOS환자성숙란모세포중란원성인자표체수평강저,가능영향세포질성숙,종이도치PCOS환자란모세포질량하강화발육잠능수손.
Objective To investigate the expression pattern and significance of two importantoocyte-secreted factors:growth differentiation factor 9(GDF9)and bone morphogenetic protein 15(BMP15)during oocyte maturation in women with polycystic ovary syndrome(PCOS)and infertile women due to husband factors.Methods Total of 25 oocytes[9 at germinal vesicle GV stage,9 at M Ⅰ stage and 7 at M Ⅱ stage]were obtained from 12 patients with PCOS and 82 oocytes(29 at GV stage,26 at M Ⅰ stage and 27 at M Ⅱ stage)were from 56 controls.The nested quantitative real time(RT)PCR was uscd to detect the abundance of GDF9 and BMP15 mRNA in each oocyte.Results(1)The expression level of GDF9 mRNA at the GV stage,M Ⅰ stage and M Ⅱ stage in PCOS group were 44.8(4.2-529.0),27.6(9.8-172.7)and 49.0(0.2-65.9)respectively,the expression in were 149.9(55.4-387.9),29.9(2.5-205.8)and 657.8(149.4-1376.2)in control group,respectively.The expression of GDF9 mRNA at M Ⅱ stage was significantly lower in PCOS group than in controls(P ＜ 0.01),however,the differences didn't reach statistical significant at GV or M Ⅰ stage between the two groups(P ＞ 0.05).The expression of GDF9 mRNA displayed some changes at different maturation stage in controls(P ＜ 0.05,P ＜ 0.01),however,the expression didn't demonstrate any dynamic changes in PCOS group(P ＞ 0.05).(2)The expression level BMP15 mRNA at the GV stage,M Ⅰ stage and M Ⅱ stage in PCOS group were 0.1(0.1-22.0),3.2(0.6-55.0)and 6.4(3.2-8.5),respectively,the expression were 41.6(6.5-96.1),4.0(2.0-10.4)and 49.7(2.3-139.5)in control group,respectively.The expression of BMP15 mRNA at GV stage was significantly lower in PCOS group than in controls(P ＜ 0.01),however,the differences were not significant at M Ⅰ or M Ⅱ stage between the two groups(P ＞0.05).The expression of BMP15 mRNA also displayed some changes at different maturation stage in controls(P ＜ 0.05),however,the level didn't demonstrate any dynamic changes in PCOS group(P ＞ 0.05).Conclusion It was suggested that the low expression of oocyte secreted factors in mature oocytes from PCOS patients might be associated with impaired oocyte quality and developmental competence in PCOS.
