中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2013年
2期
107-112
,共6页
吴晓霞%陈颖%谭剑平%刘梅兰%张建平
吳曉霞%陳穎%譚劍平%劉梅蘭%張建平
오효하%진영%담검평%류매란%장건평
肝素,低分子量%胞间信号肽类和蛋白质类%滋养层%妊娠初期%细胞增殖%细胞分化
肝素,低分子量%胞間信號肽類和蛋白質類%滋養層%妊娠初期%細胞增殖%細胞分化
간소,저분자량%포간신호태류화단백질류%자양층%임신초기%세포증식%세포분화
Heparin,low-molecular-weight%Intercellular signaling peptides and proteins%Trophoblasts%Pregnancy trimester,first%Cell proliferation%Cell differentiation
目的 观察低分子肝素及肝素结合型表皮生长因子(HB-EGF)对孕早期滋养细胞功能的影响.方法 2011年2月至11月在中山大学附属第二医院进行体外培养孕早期绒毛组织中的滋养细胞.按照不同浓度的低分子肝素分为0.025 U/ml组、0.25 U/ml组、2.5 U/ml组、25 U/ml组、250 U/ml组；按照低分子肝素、HB-EGF单独及联合用药,分为低分子肝素组(浓度0.25 U/ml),HB-EGF组(浓度10 μg/L),联合用药组(浓度0.25 U/ml的低分子肝素+10 μg/L的HB-EGF)；将加入DMEM设为对照组.采用四甲基偶氮唑蓝比色法检测孕早期滋养细胞增殖能力,以平均吸光度(A)值表示；采用穿膜小室实验检测孕早期滋养细胞的侵袭能力,以穿透至膜下细胞数表示；并检测培养上清液中孕早期滋养细胞分泌的hCG水平以评价滋养细胞的分化能力.结果 与对照组比较,低分子肝素浓度为0.025 U/ml对孕早期滋养细胞增殖、侵袭能力影响不明显(P＞0.05)；低分子肝素浓度为0.25 U/ml、2.5 U/ml时,低分子肝素增加孕早期滋养细胞增殖、侵袭能力(P＜0.05)；低分子肝素浓度为25 U/ml、250 U/ml时,则低分子肝素显著抑制孕早期滋养细胞增殖、侵袭能力(P＜0.05).与对照组A值(0.44±0.04)比较,低分子肝素组(A值为0.51 ±0.05)、HB-EGF组(A值为0.56±0.04)、联合用药组(A值为0.69±0.06)均显著增高(P＜0.05).在侵袭试验中,低分子肝素组侵袭细胞数为(511±78)个,HB-EGF组为(669±67)个,联合用药组为(872±64)个,分别与对照组(405±67)个比较,差异均有统计学意义(P＜0.05).低分子肝素组上清液中hCG水平为(7143±649)U/L,HB-EGF组为(11 762±1059)U/L,联合用药组为(11 015±1084)U/L,分别与对照组(8182±666)U/L比较,差异也均有统计学意义(P＜0.05).结论 低分子肝素可以调节孕早期滋养细胞的增殖、侵袭和分化能力.HB-EGF是低分子肝素对孕早期滋养细胞产生作用的一个重要因素.
目的 觀察低分子肝素及肝素結閤型錶皮生長因子(HB-EGF)對孕早期滋養細胞功能的影響.方法 2011年2月至11月在中山大學附屬第二醫院進行體外培養孕早期絨毛組織中的滋養細胞.按照不同濃度的低分子肝素分為0.025 U/ml組、0.25 U/ml組、2.5 U/ml組、25 U/ml組、250 U/ml組；按照低分子肝素、HB-EGF單獨及聯閤用藥,分為低分子肝素組(濃度0.25 U/ml),HB-EGF組(濃度10 μg/L),聯閤用藥組(濃度0.25 U/ml的低分子肝素+10 μg/L的HB-EGF)；將加入DMEM設為對照組.採用四甲基偶氮唑藍比色法檢測孕早期滋養細胞增殖能力,以平均吸光度(A)值錶示；採用穿膜小室實驗檢測孕早期滋養細胞的侵襲能力,以穿透至膜下細胞數錶示；併檢測培養上清液中孕早期滋養細胞分泌的hCG水平以評價滋養細胞的分化能力.結果 與對照組比較,低分子肝素濃度為0.025 U/ml對孕早期滋養細胞增殖、侵襲能力影響不明顯(P＞0.05)；低分子肝素濃度為0.25 U/ml、2.5 U/ml時,低分子肝素增加孕早期滋養細胞增殖、侵襲能力(P＜0.05)；低分子肝素濃度為25 U/ml、250 U/ml時,則低分子肝素顯著抑製孕早期滋養細胞增殖、侵襲能力(P＜0.05).與對照組A值(0.44±0.04)比較,低分子肝素組(A值為0.51 ±0.05)、HB-EGF組(A值為0.56±0.04)、聯閤用藥組(A值為0.69±0.06)均顯著增高(P＜0.05).在侵襲試驗中,低分子肝素組侵襲細胞數為(511±78)箇,HB-EGF組為(669±67)箇,聯閤用藥組為(872±64)箇,分彆與對照組(405±67)箇比較,差異均有統計學意義(P＜0.05).低分子肝素組上清液中hCG水平為(7143±649)U/L,HB-EGF組為(11 762±1059)U/L,聯閤用藥組為(11 015±1084)U/L,分彆與對照組(8182±666)U/L比較,差異也均有統計學意義(P＜0.05).結論 低分子肝素可以調節孕早期滋養細胞的增殖、侵襲和分化能力.HB-EGF是低分子肝素對孕早期滋養細胞產生作用的一箇重要因素.
목적 관찰저분자간소급간소결합형표피생장인자(HB-EGF)대잉조기자양세포공능적영향.방법 2011년2월지11월재중산대학부속제이의원진행체외배양잉조기융모조직중적자양세포.안조불동농도적저분자간소분위0.025 U/ml조、0.25 U/ml조、2.5 U/ml조、25 U/ml조、250 U/ml조；안조저분자간소、HB-EGF단독급연합용약,분위저분자간소조(농도0.25 U/ml),HB-EGF조(농도10 μg/L),연합용약조(농도0.25 U/ml적저분자간소+10 μg/L적HB-EGF)；장가입DMEM설위대조조.채용사갑기우담서람비색법검측잉조기자양세포증식능력,이평균흡광도(A)치표시；채용천막소실실험검측잉조기자양세포적침습능력,이천투지막하세포수표시；병검측배양상청액중잉조기자양세포분비적hCG수평이평개자양세포적분화능력.결과 여대조조비교,저분자간소농도위0.025 U/ml대잉조기자양세포증식、침습능력영향불명현(P＞0.05)；저분자간소농도위0.25 U/ml、2.5 U/ml시,저분자간소증가잉조기자양세포증식、침습능력(P＜0.05)；저분자간소농도위25 U/ml、250 U/ml시,칙저분자간소현저억제잉조기자양세포증식、침습능력(P＜0.05).여대조조A치(0.44±0.04)비교,저분자간소조(A치위0.51 ±0.05)、HB-EGF조(A치위0.56±0.04)、연합용약조(A치위0.69±0.06)균현저증고(P＜0.05).재침습시험중,저분자간소조침습세포수위(511±78)개,HB-EGF조위(669±67)개,연합용약조위(872±64)개,분별여대조조(405±67)개비교,차이균유통계학의의(P＜0.05).저분자간소조상청액중hCG수평위(7143±649)U/L,HB-EGF조위(11 762±1059)U/L,연합용약조위(11 015±1084)U/L,분별여대조조(8182±666)U/L비교,차이야균유통계학의의(P＜0.05).결론 저분자간소가이조절잉조기자양세포적증식、침습화분화능력.HB-EGF시저분자간소대잉조기자양세포산생작용적일개중요인소.
Objective To evaluate the effects of low molecular weight heparin(LMWH)and heparin-binding epidermal growth factor(HB-EGF)on the biological function of human trophoblast in first trimester.Methods From Feb.2011 to Nov.2011,the trophoblast isolated from human first trimester chorionic villi was cultured in vitro.Based on variation of LMWH concentration,the trophoblast was classified into 0.025 U/ml group,0.25 U/ml group,2.5 U/ml group,25 U/ml group and 250 U/ml group.In the mean time,based on treatment of heparin,the trophoblast was classified into LMWH group (0.25 U/ml),HB-EGF group(10 μg/L),combination group(LMWH at 0.25 U/ml + HB-EGF at 10 μg/L)and add with DMEM as control group.Cell prolferation was assessed by the methyl thiazolyl tetrazolium(MTY)test,which was showed with the mean absorbance as A value.Cell invasion was measured by transwell,which counted the number of cells migrated to the superficies inferia of filter membrane.Cell differentiation was assessed by the concentration of hCG secretion.Results Compared with control group,the trophoblast proliferation and invasion treated by LMWH at 0.025 U/ml did not show significant difference (P ＞ 0.05).When treated by LWMH at 0.25 U/ml and 2.5 U/ml,trophoblast proliferation and invasion was increased significantly(P ＜ 0.05).When LMWH at 25 U/ml and 250 U/ml,it could inhibit trophoblast proliferation and invasion(P ＜ 0.05).When compared with A value of 0.44 ± 0.04 in control group,the increased A value were 0.51 ± 0.05 in LMWH group,0.56 ± 0.04 in HB-EGF group and 0.69 ± 0.06 in combination group(P ＜ 0.05).In the transwell test,the cell number were 511 ± 78 in LMWH group,669 ± 67 in HB-EGF group and 872±64 in combination group,which were significantly higher than 405 ± 67 in control group(P ＜ 0.05),respectively.And the hCG concentration were(7143 ± 649)U/L in LMWH group,(11 762 ± 1059)U/L in HB-EGF group and(11 015 ± 1084)U/L in combination group,which showed statistical difference with(8182 ± 666)U/L in control group(P ＜ 0.05).Conclusion LMWH could modulate trophoblast proliferation,invasion,and differentiation.HB-EGF is one of important factors involved in effects of LMWH on trophoblast function.
