CAJ | 학술논문

目的探讨外阴鳞癌组织中E钙黏蛋白(E-cadherin)和β连环蛋白(β-catenin)的表达及其意义.方法收集2005年1月至2012年4月在中国医科大学附属盛京医院妇产科行手术治疗的不同外阴病变患者的石蜡包埋标本63份,其中外阴鳞癌41份(外阴鳞癌组),外阴鳞状上皮内瘤变(VIN)Ⅱ～Ⅲ22份(VINⅡ～Ⅲ组)；以其中10份外阴鳞癌患者术后病理检查证实其切缘阴性的正常外阴组织标本作为对照组.采用免疫组化EliVision二步法检测3组标本中E-cadherin和β-catenin 蛋白的表达情况,并对E-cadherin和β-catenin蛋白的表达与外阴鳞癌患者绝经状态、临床分期、病理分级及淋巴结转移的关系进行分析；采用Spearman相关分析法对外阴鳞癌、VINⅡ～Ⅲ组织中E-cadherin与β-catenin蛋白的表达进行相关性分析.结果免疫组化EliVision二步法检测显示,外阴鳞癌组E-cadherin和β-catenin蛋白的异常表达率分别为46％(19/41)和61％ (25/41),VINⅡ～Ⅲ组分别为64％(14/22)和68％(15/22),均明显高于对照组(均为0),差异均有统计学意义(P＜0.05)；而外阴鳞癌组与VINⅡ～Ⅲ组比较,差异无统计学意义(P＞0.05)；E-cadherin和β-catenin蛋白的异常表达率与外阴鳞癌患者的病理分级及淋巴结转移有关(P＜0.05),而与绝经状态及临床分期无关(P＞0.05).Spearman相关分析显示,外阴鳞癌组41份组织中,E-cadherin和β-catenin蛋白均正常表达者14份,均异常表达者17份,两者的表达呈明显正相关关系(r=0.543,P=0.000)；VINⅡ～Ⅲ组22份组织中,E-cadherin和β-catenin蛋白均正常表达者4份,均异常表达者11份,两者的表达无明显相关关系(r=0.295,P=0.182).结论外阴鳞癌组织中E-cadherin和β-catenin蛋白存在异常表达,其异常表达与外阴鳞癌的病理分级及淋巴结转移相关,可能参与外阴鳞癌的发生、发展.
목적 탐토외음린암조직중E개점단백(E-cadherin)화β련배단백(β-catenin)적표체급기의의.방법 수집2005년1월지2012년4월재중국의과대학부속성경의원부산과행수술치료적불동외음병변환자적석사포매표본63빈,기중외음린암41빈(외음린암조),외음린상상피내류변(VIN)Ⅱ～Ⅲ22빈(VINⅡ～Ⅲ조)；이기중10빈외음린암환자술후병리검사증실기절연음성적정상외음조직표본작위대조조.채용면역조화EliVision이보법검측3조표본중E-cadherin화β-catenin 단백적표체정황,병대E-cadherin화β-catenin단백적표체여외음린암환자절경상태、림상분기、병리분급급림파결전이적관계진행분석；채용Spearman상관분석법대외음린암、VINⅡ～Ⅲ조직중E-cadherin여β-catenin단백적표체진행상관성분석.결과 면역조화EliVision이보법검측현시,외음린암조E-cadherin화β-catenin단백적이상표체솔분별위46％(19/41)화61％ (25/41),VINⅡ～Ⅲ조분별위64％(14/22)화68％(15/22),균명현고우대조조(균위0),차이균유통계학의의(P＜0.05)；이외음린암조여VINⅡ～Ⅲ조비교,차이무통계학의의(P＞0.05)；E-cadherin화β-catenin단백적이상표체솔여외음린암환자적병리분급급림파결전이유관(P＜0.05),이여절경상태급림상분기무관(P＞0.05).Spearman상관분석현시,외음린암조41빈조직중,E-cadherin화β-catenin단백균정상표체자14빈,균이상표체자17빈,량자적표체정명현정상관관계(r=0.543,P=0.000)；VINⅡ～Ⅲ조22빈조직중,E-cadherin화β-catenin단백균정상표체자4빈,균이상표체자11빈,량자적표체무명현상관관계(r=0.295,P=0.182).결론 외음린암조직중E-cadherin화β-catenin단백존재이상표체,기이상표체여외음린암적병리분급급림파결전이상관,가능삼여외음린암적발생、발전.
Objective To iuvestigate the expression of E-cadherin and β-catenin in the Vulvar squamous cell carcinoma(VSCC) tissues.Methods A total of 63 documented paraffin blocks of VSCC (n =41),vulvar intraepithelial neoplasia (n =22),vulvar negative cutting edge tissues (n =10) diagnosed in department of pathology of Shengjing Hospital of China Medical University from January 2005 to April 2012 were enrolled.EliVision immunohistochemical staining was used to detect the expression of E-cadherin and β-catenin in the three groups.Then to do a statistical analysis among the expression of them with patients' menopause status,pathological grade,clinical stage and lymph node metastasis.Spearman correlation analysis was used to analyse the expression of E-cadherin and β-catenin in the vulvar lesion tissues.Results The abnurmal immunoreactivity for E-cadherin [46％ (19/41),64％ (14/22)] and β-catenin[61％ (25/41),68％ (15/22)] in VSCC and VIN Ⅱ-Ⅲ were found,which were significantly different from that in normal epithelium samples (P ＜ 0.05).The abnormal expression of E-cadherin and β-catenin have no statistically significant difference between VSCC group and VIN Ⅱ-Ⅲ group (P ＞0.05).The abnormal expression of E-cadherin and β-catenin were collected with tumor pathological grade and lymph node metastasis status(all P ＜ 0.05).The abnormal expression of E-cadherin and β-catenin have no statistically significant difference between menopause and the surgical stage of patients (all P ＞0.05).The abnormal expression of E-cadherin and β-catenin have a significant positive correlation in the same sample in the VSCC tissue (r =0.543,P =0.000).The abnormal expression of E-cadherin and β-catenin have no correlation in the VIN Ⅱ-Ⅲ tissue (r =0.295,P =0.182).Conclusions The abnormal expression of E-cadherin and β-catenin may occurs frequently in the VSCC.The abnormal expression of E-cadherin and β-catenin have correlation with vulvar cancer pathological grade and lymph node metastasis,which may be important mechanisms promoting the invasion and metastasis of VSCC.