中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2013年
7期
528-531
,共4页
张玮%杨莹珠%李力%王琪
張瑋%楊瑩珠%李力%王琪
장위%양형주%리력%왕기
卵巢肿瘤%细胞系,肿瘤%趋化因子,CC%肿瘤转移
卵巢腫瘤%細胞繫,腫瘤%趨化因子,CC%腫瘤轉移
란소종류%세포계,종류%추화인자,CC%종류전이
Ovarian neoplasms%Cell line,tumor%Chemokines,CC%Neoplasm metastasis
目的 探讨C-C型趋化因子配体18(CCL18)过表达对卵巢上皮性癌(卵巢癌)细胞生长和浸润转移的影响.方法 构建CCL18重组表达载体并介导SKOV3细胞过表达CCL18,分别采用四甲基偶氮唑蓝(MTT)法、流式细胞仪、穿膜小室(transwell小室)迁移、侵袭和纤连蛋白黏附方法测定SKOV3细胞(实验细胞为SKOV3-CCL18,以SKOV3-pEGFP-N1为对照细胞)过表达CCL18后细胞生长曲线、细胞生长周期以及细胞体外迁移、侵袭、黏附能力的变化.结果 (1)成功构建CCL18-pEGFP-N1重组质粒并转染至SKOV3细胞,筛选和建成稳定生长的SKOV3-CCL18细胞.(2)SKOV3-CCL18细胞与空白SKOV3、SKOV3-pEGFP-N1细胞比较,生长曲线差异无统计学意义(P均＞0.05)；但SKOV3-CCL18细胞处于增殖状态(S+G2 +M期)的比例明显高于SKOV3、SKOV3-pEGFP-N1细胞(分别为32.80％、27.06％、26.98％),分别比较,差异均有统计学意义(P＜0.05).(3)SKOV3-CCL18细胞的体外侵袭、迁移和黏附能力(分别为0.49±0.18、1.16±0.25和0.39±0.10)均明显高于空白SKOV3细胞(分别为0.23 ±0.13、0.36±0.10、0.16±0.03)和SKOV3-pEGFP-N1细胞(分别为0.19±0.05,0.38±0.23和0.13±0.11),分别比较,差异均有统计学意义(P＜0.05).结论 卵巢癌SKOV3细胞CCL18过表达导致其体外侵袭、迁移和黏附能力增强.
目的 探討C-C型趨化因子配體18(CCL18)過錶達對卵巢上皮性癌(卵巢癌)細胞生長和浸潤轉移的影響.方法 構建CCL18重組錶達載體併介導SKOV3細胞過錶達CCL18,分彆採用四甲基偶氮唑藍(MTT)法、流式細胞儀、穿膜小室(transwell小室)遷移、侵襲和纖連蛋白黏附方法測定SKOV3細胞(實驗細胞為SKOV3-CCL18,以SKOV3-pEGFP-N1為對照細胞)過錶達CCL18後細胞生長麯線、細胞生長週期以及細胞體外遷移、侵襲、黏附能力的變化.結果 (1)成功構建CCL18-pEGFP-N1重組質粒併轉染至SKOV3細胞,篩選和建成穩定生長的SKOV3-CCL18細胞.(2)SKOV3-CCL18細胞與空白SKOV3、SKOV3-pEGFP-N1細胞比較,生長麯線差異無統計學意義(P均＞0.05)；但SKOV3-CCL18細胞處于增殖狀態(S+G2 +M期)的比例明顯高于SKOV3、SKOV3-pEGFP-N1細胞(分彆為32.80％、27.06％、26.98％),分彆比較,差異均有統計學意義(P＜0.05).(3)SKOV3-CCL18細胞的體外侵襲、遷移和黏附能力(分彆為0.49±0.18、1.16±0.25和0.39±0.10)均明顯高于空白SKOV3細胞(分彆為0.23 ±0.13、0.36±0.10、0.16±0.03)和SKOV3-pEGFP-N1細胞(分彆為0.19±0.05,0.38±0.23和0.13±0.11),分彆比較,差異均有統計學意義(P＜0.05).結論 卵巢癌SKOV3細胞CCL18過錶達導緻其體外侵襲、遷移和黏附能力增彊.
목적 탐토C-C형추화인자배체18(CCL18)과표체대란소상피성암(란소암)세포생장화침윤전이적영향.방법 구건CCL18중조표체재체병개도SKOV3세포과표체CCL18,분별채용사갑기우담서람(MTT)법、류식세포의、천막소실(transwell소실)천이、침습화섬련단백점부방법측정SKOV3세포(실험세포위SKOV3-CCL18,이SKOV3-pEGFP-N1위대조세포)과표체CCL18후세포생장곡선、세포생장주기이급세포체외천이、침습、점부능력적변화.결과 (1)성공구건CCL18-pEGFP-N1중조질립병전염지SKOV3세포,사선화건성은정생장적SKOV3-CCL18세포.(2)SKOV3-CCL18세포여공백SKOV3、SKOV3-pEGFP-N1세포비교,생장곡선차이무통계학의의(P균＞0.05)；단SKOV3-CCL18세포처우증식상태(S+G2 +M기)적비례명현고우SKOV3、SKOV3-pEGFP-N1세포(분별위32.80％、27.06％、26.98％),분별비교,차이균유통계학의의(P＜0.05).(3)SKOV3-CCL18세포적체외침습、천이화점부능력(분별위0.49±0.18、1.16±0.25화0.39±0.10)균명현고우공백SKOV3세포(분별위0.23 ±0.13、0.36±0.10、0.16±0.03)화SKOV3-pEGFP-N1세포(분별위0.19±0.05,0.38±0.23화0.13±0.11),분별비교,차이균유통계학의의(P＜0.05).결론 란소암SKOV3세포CCL18과표체도치기체외침습、천이화점부능력증강.
Objective To investigate the effect of growth,invasion and metastasis on ovarian cancer cell line SKOV3 with chemokine (C-C motif) ligand 18 (CCL18) over-expression by mediated in vitro.Methods The restructuring plasmid of CCL18 expression was constructed and SKOV3 cells was transfected with plasmid DNA in vitro.The growth curve,cell cycle,cell migration,invasion and adhesion capacity in SKOV3-CCL18 cells and control cells were detected by methyl thiazolyl tetrazolium (MTT) assay,flow cytometry,transwell chamber,migration invasion and fibronectin adhesion method,respectively.Results (1) CCL18-pEGFP-N1 plasmid was successfully constructed and transmitted to the SKOV3 cells,the stable growth of the subculture SKOV3-CCL18 cells was screening and completed.(2) Compared with SKOV3-CCL18 cells and SKOV3 or SKOV3-pEGFP-N1 cells,there was not differences statistically significant in growth curve (all P ＞ 0.05) ; but the rate of the SKOV3-CCL18 cells in a proliferative field (S + G2 + M) was significantly higher than that in SKOV3 or SKOV3-pEGFP-N1 cells (32.80％ versus 27.06％,32.80％ versus 26.98％,respectively; all P ＜ 0.05).(3) The invasion,migration and adhesion capacity of SKOV3-CCL18 cells (being 0.49 ± 0.18,1.16 ± 0.25 and 0.39 ± 0.10,respectively) in vitro were significantly higher than those in SKOV3 (being 0.23 ± 0.13,0.36 ± 0.10 and 0.16 ± 0.03,respectively) or SKOV3-pEGFP-N 1 cells (being 0.19 ± 0.05,0.38 ± 0.23 and 0.13 ± 0.11,respectively; all P ＜ 0.05).Conclusion The CCL18 over-expression in epithelial ovarian cancer SKOV3 cells could lead to strengthen ability of invasion,migration and adhesion in vitro.
