中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2013年
8期
607-610
,共4页
宫颈肿瘤%癌前状态%人乳头瘤病毒16%甲基化%序列分析,DNA
宮頸腫瘤%癌前狀態%人乳頭瘤病毒16%甲基化%序列分析,DNA
궁경종류%암전상태%인유두류병독16%갑기화%서렬분석,DNA
Uterine cervical neoplasms%Precancerous conditions%Human papillomavirus 16%Methylation%Sequence analysis,DNA
目的 定位、定量检测位于HPV16 L1基因3’端和LCR基因的甲基化位点的甲基化水平,了解HPV16甲基化与其致病性的关系.方法 选择HPV16阳性的无宫颈病变携带者、宫颈上皮内瘤变(CIN)患者和宫颈癌患者各30例,分别命名为携带组、CIN组、宫颈癌组,使用宫颈液基细胞学检查后剩余的细胞提取DNA,经处理后,扩增HPV16 L1基因3’端和LCR基因,采用焦磷酸测序技术检测HPV16 L1基因3’端和LCR基因的21个位点的甲基化状况,比较每个位点的甲基化水平是否存在差异.结果 位于E6/E7启动子的5个位点31、37、43、52、58均存在随病情进展甲基化水平逐渐降低的趋势,宫颈癌组患者的甲基化水平最低(分别为21.86％、28.15％、21.37％、26.15％、15.48％),分别比较,差异均有统计学意义(P＜0.01).位于L1基因3’端的7032、7091、7136 3个位点的甲基化水平在携带组、CIN组和宫颈癌组患者间比较,差异有统计学意义(P均＜0.01)；7032、7091位点携带组的甲基化水平最低(分别为2.71％、6.95％),宫颈癌组甲基化水平最高(分别为18.89％、27.72％);7136位点CIN组患者的甲基化水平最高(66.45％),宫颈癌组次之(46.43％),携带组最低(34.85％).结论 HPV16 L1基因3’端及E6/E7启动子甲基化位点的甲基化水平存在显著性差异,HPV16甲基化状况有可能预测宫颈癌癌前病变或宫颈癌的发生.
目的 定位、定量檢測位于HPV16 L1基因3’耑和LCR基因的甲基化位點的甲基化水平,瞭解HPV16甲基化與其緻病性的關繫.方法 選擇HPV16暘性的無宮頸病變攜帶者、宮頸上皮內瘤變(CIN)患者和宮頸癌患者各30例,分彆命名為攜帶組、CIN組、宮頸癌組,使用宮頸液基細胞學檢查後剩餘的細胞提取DNA,經處理後,擴增HPV16 L1基因3’耑和LCR基因,採用焦燐痠測序技術檢測HPV16 L1基因3’耑和LCR基因的21箇位點的甲基化狀況,比較每箇位點的甲基化水平是否存在差異.結果 位于E6/E7啟動子的5箇位點31、37、43、52、58均存在隨病情進展甲基化水平逐漸降低的趨勢,宮頸癌組患者的甲基化水平最低(分彆為21.86％、28.15％、21.37％、26.15％、15.48％),分彆比較,差異均有統計學意義(P＜0.01).位于L1基因3’耑的7032、7091、7136 3箇位點的甲基化水平在攜帶組、CIN組和宮頸癌組患者間比較,差異有統計學意義(P均＜0.01)；7032、7091位點攜帶組的甲基化水平最低(分彆為2.71％、6.95％),宮頸癌組甲基化水平最高(分彆為18.89％、27.72％);7136位點CIN組患者的甲基化水平最高(66.45％),宮頸癌組次之(46.43％),攜帶組最低(34.85％).結論 HPV16 L1基因3’耑及E6/E7啟動子甲基化位點的甲基化水平存在顯著性差異,HPV16甲基化狀況有可能預測宮頸癌癌前病變或宮頸癌的髮生.
목적 정위、정량검측위우HPV16 L1기인3’단화LCR기인적갑기화위점적갑기화수평,료해HPV16갑기화여기치병성적관계.방법 선택HPV16양성적무궁경병변휴대자、궁경상피내류변(CIN)환자화궁경암환자각30례,분별명명위휴대조、CIN조、궁경암조,사용궁경액기세포학검사후잉여적세포제취DNA,경처리후,확증HPV16 L1기인3’단화LCR기인,채용초린산측서기술검측HPV16 L1기인3’단화LCR기인적21개위점적갑기화상황,비교매개위점적갑기화수평시부존재차이.결과 위우E6/E7계동자적5개위점31、37、43、52、58균존재수병정진전갑기화수평축점강저적추세,궁경암조환자적갑기화수평최저(분별위21.86％、28.15％、21.37％、26.15％、15.48％),분별비교,차이균유통계학의의(P＜0.01).위우L1기인3’단적7032、7091、7136 3개위점적갑기화수평재휴대조、CIN조화궁경암조환자간비교,차이유통계학의의(P균＜0.01)；7032、7091위점휴대조적갑기화수평최저(분별위2.71％、6.95％),궁경암조갑기화수평최고(분별위18.89％、27.72％);7136위점CIN조환자적갑기화수평최고(66.45％),궁경암조차지(46.43％),휴대조최저(34.85％).결론 HPV16 L1기인3’단급E6/E7계동자갑기화위점적갑기화수평존재현저성차이,HPV16갑기화상황유가능예측궁경암암전병변혹궁경암적발생.
Objective Quantifiably and located measure the methylation rate of 21 cytosinephosphate-guanosine (CpG) sites in the 3' region of L1 gene and long control region (LCR) gene of HPV16 DNA in asymptomatic patients,cervical intraepithelial neoplasia (CIN) patients,and cervical cancer patients.To analysis the relationship between HPV16 methylation and it's pathogenicity.Methods Chosen 30 cases with HPV16 positive in each group.Firstly,extract DNA from the remaining cells of liquid-based cytology specimen and bisulfite treatment DNA,then amplify the 3' region of L1 gene and LCR gene,test the methylation rate of 21 CpG sites of HPV16 DNA in three groups.Results All of the 5 CpG sites in E6/E7 promoter (31,37,43,52,58) were hypomethylation in cervical cancer group (21.86％,28.15％,21.37％,26.15％,15.48％,respectively),hypermethylation in asymptomatic group,and middle-methylation in CIN group,in which there were significant difference among three groups (all P ＜0.01).The CpG site in 7032,7091,7136 of the 3' region of L1 gene was also different methylated among three groups (all P＜0.01).Hypermethylation was found in cancer group (18.89％,27.72％),hypomethylation was found in asymptomatic group (2.71％,6.95％) in 7032 and 7091.In 7136,the highest methylation was detected in CIN (66.45％),the lowest in asymptomatic (34.85％),middle in cancer group (46.43％).Conclusion The methylation status of CpG sites in the 3' region of L1 gene and E6/E7 promoter of HPV16 is significant different among three groups,which is likely to anticipate the pathogenesis of CIN and cervical cancer.
