中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2013年
10期
759-762
,共4页
许银燕%丁卉%张芬芬%王俐
許銀燕%丁卉%張芬芬%王俐
허은연%정훼%장분분%왕리
先兆子痫%滋养层%自噬%微管相关蛋白质类%半胱氨酸内肽酶类
先兆子癇%滋養層%自噬%微管相關蛋白質類%半胱氨痠內肽酶類
선조자간%자양층%자서%미관상관단백질류%반광안산내태매류
Pre-eclampsia%Trophoblasts%Autophagy%Microtubule-associated proteins%Cysteine endopeptidases
目的 探讨子痫前期(PE)患者胎盘滋养细胞自噬的发生规律及其可能的分子机制.方法 2011年11月至2012年4月在南京妇幼保健院收集20例PE患者(PE组)及20例正常妊娠妇女(对照组)的胎盘组织,透射电镜观察滋养细胞自噬体的形成情况；蛋白印迹法和实时荧光定量PCR技术检测胎盘组织中自噬相关基因Atg4B、微管相关蛋白l轻链3(LC3)Ⅱ、Ⅰ蛋白和mRNA的表达变化.结果 与对照组孕妇相比,PE组孕妇胎盘组织在透射电镜下见滋养细胞胞质中典型的自噬体形成.蛋白印迹法和实时荧光定量PCR技术检测结果显示,PE组患者胎盘组织中LC3-Ⅱ/Ⅰ比值(1.43±0.23)高于对照组(0.59 ±0.12),两组比较,差异有统计学意义(P ＜0.05)；Atg4B蛋白的相对表达量(0.71±0.13)高于对照组(0.33±0.07),两组比较,差异也有统计学意义(P ＜0.05)；PE组Atg4B mRNA的表达水平上调,为对照组的(1.73±0.16)倍,两组比较,差异有统计学意义(P＜0.05).结论 PE患者滋养细胞白噬活性的增高,可能参与了PE的发生发展.
目的 探討子癇前期(PE)患者胎盤滋養細胞自噬的髮生規律及其可能的分子機製.方法 2011年11月至2012年4月在南京婦幼保健院收集20例PE患者(PE組)及20例正常妊娠婦女(對照組)的胎盤組織,透射電鏡觀察滋養細胞自噬體的形成情況；蛋白印跡法和實時熒光定量PCR技術檢測胎盤組織中自噬相關基因Atg4B、微管相關蛋白l輕鏈3(LC3)Ⅱ、Ⅰ蛋白和mRNA的錶達變化.結果 與對照組孕婦相比,PE組孕婦胎盤組織在透射電鏡下見滋養細胞胞質中典型的自噬體形成.蛋白印跡法和實時熒光定量PCR技術檢測結果顯示,PE組患者胎盤組織中LC3-Ⅱ/Ⅰ比值(1.43±0.23)高于對照組(0.59 ±0.12),兩組比較,差異有統計學意義(P ＜0.05)；Atg4B蛋白的相對錶達量(0.71±0.13)高于對照組(0.33±0.07),兩組比較,差異也有統計學意義(P ＜0.05)；PE組Atg4B mRNA的錶達水平上調,為對照組的(1.73±0.16)倍,兩組比較,差異有統計學意義(P＜0.05).結論 PE患者滋養細胞白噬活性的增高,可能參與瞭PE的髮生髮展.
목적 탐토자간전기(PE)환자태반자양세포자서적발생규률급기가능적분자궤제.방법 2011년11월지2012년4월재남경부유보건원수집20례PE환자(PE조)급20례정상임신부녀(대조조)적태반조직,투사전경관찰자양세포자서체적형성정황；단백인적법화실시형광정량PCR기술검측태반조직중자서상관기인Atg4B、미관상관단백l경련3(LC3)Ⅱ、Ⅰ단백화mRNA적표체변화.결과 여대조조잉부상비,PE조잉부태반조직재투사전경하견자양세포포질중전형적자서체형성.단백인적법화실시형광정량PCR기술검측결과현시,PE조환자태반조직중LC3-Ⅱ/Ⅰ비치(1.43±0.23)고우대조조(0.59 ±0.12),량조비교,차이유통계학의의(P ＜0.05)；Atg4B단백적상대표체량(0.71±0.13)고우대조조(0.33±0.07),량조비교,차이야유통계학의의(P ＜0.05)；PE조Atg4B mRNA적표체수평상조,위대조조적(1.73±0.16)배,량조비교,차이유통계학의의(P＜0.05).결론 PE환자자양세포백서활성적증고,가능삼여료PE적발생발전.
Objective To investigate the occurrence law of autophagy in trophoblast cells from preeclampsia and its underlying mechanisms.Methods Twenty cases of placenta tissues were collected from women suffered from preeclampsia and normal pregnant women respectively.Autophagosome of trophoblast cells were observed by transmission electron microscope.The expressions of LC3-Ⅱ/Ⅰ and Atg4B in placenta tissues were detected by western blot and real-time PCR.Results Compared with the control group,typical autophagosomes of trophoblast cells were observed by transmission electron microscope.The ratio of LC3-Ⅱ / Ⅰ in placenta of PE patients was increased (1.43 ± 0.23) compared with control group (0.59 ±0.12),and the expression of Atg4B was up-regulated in both mRNA [(1.73 ±0.16) folds] and protein levels (0.71 ± 0.13) compared with control group (P ＜ 0.05).Conclusions Autophagy was significantly up-regulated in trophoblast cells from patients suffered from preeclampsia.Thus,all the data suggest that autophagy might be involved in the generation of preeclampsia.
