CAJ | 학술논문

目的探讨山西汉族人群血清可溶性人白细胞抗原-Ⅰ (sHLA-Ⅰ)水平与类风湿关节炎(RA)的关系.方法酶联免疫吸附测定(ELISA)法检测血清sHLA-Ⅰ类抗原水平.将不同浓度的标准品和待检血清分别加人包被有特异性sHLA-Ⅰ抗体的酶标板,与生物素化的sHLA-Ⅰ、辣根过氧化物酶(HRP)标记的链霉亲和素共同孵育,弃上清并用洗涤液洗涤,加底物应用液四甲基联苯胺(TMB)显色,以酶标仪450 nm测定吸光度(A)值,根据不同浓度标准品显色后测得的A值绘制标准曲线.测定RA患者与健康人的sHLA-Ⅰ含量.结果 ①血清sHLA-Ⅰ类抗原水平:80名健康人为(390±106)ng/ml;80例RA患者为(632±169)ng/ml,其中50例活动期RA患者为(739±144)ng/ml,30例缓解期RA患者为(524±114)ng/ml,与健康人相比,活动期和缓解期RA患者血清sHLA-Ⅰ均显著增高(P＜0.01),且活动期RA患者显著高于缓解期患者(P＜0.0).②26例活动期RA患者治疗前血清sHLA-Ⅰ类抗原水平为(744±148)ng/ml,经治疗病情缓解后为(533±106)ng/ml,治疗前显著高于治疗后(P＜0.01).结论 RA患者血清sHLA-Ⅰ类抗原水平明显增高,且以活动期增高更为显著.
목적 탐토산서한족인군혈청가용성인백세포항원-Ⅰ (sHLA-Ⅰ)수평여류풍습관절염(RA)적관계.방법 매련면역흡부측정(ELISA)법검측혈청sHLA-Ⅰ류항원수평.장불동농도적표준품화대검혈청분별가인포피유특이성sHLA-Ⅰ항체적매표판,여생물소화적sHLA-Ⅰ、랄근과양화물매(HRP)표기적련매친화소공동부육,기상청병용세조액세조,가저물응용액사갑기련분알(TMB)현색,이매표의450 nm측정흡광도(A)치,근거불동농도표준품현색후측득적A치회제표준곡선.측정RA환자여건강인적sHLA-Ⅰ함량.결과 ①혈청sHLA-Ⅰ류항원수평:80명건강인위(390±106)ng/ml;80례RA환자위(632±169)ng/ml,기중50례활동기RA환자위(739±144)ng/ml,30례완해기RA환자위(524±114)ng/ml,여건강인상비,활동기화완해기RA환자혈청sHLA-Ⅰ균현저증고(P＜0.01),차활동기RA환자현저고우완해기환자(P＜0.0).②26례활동기RA환자치료전혈청sHLA-Ⅰ류항원수평위(744±148)ng/ml,경치료병정완해후위(533±106)ng/ml,치료전현저고우치료후(P＜0.01).결론 RA환자혈청sHLA-Ⅰ류항원수평명현증고,차이활동기증고경위현저.
Objective To investigate the relationship between the quantity of soluble human leukocyte antigen class(sHLA)-Ⅰ and rheumatoid arthritis(RA).Methods Enzyme-linked iromunosorhent assay (EUSA) was used to measure the level of sHLA-Ⅰ.Microtitration wells were coated with sHLA-Ⅰ antibody of defined and unique epitope specificity.Standards and corresponding samples were added into different microtitration wells respectively.and then biotinylated sHLA-Ⅰ and streptavidin labeled with enzyme horseradish pemxidase(HRP)were added into each well.Afier incubation and washing.a TMB substrate solution Waft added to the wells and color developed in proportion to the amount of sHLA-Ⅰ bound.The intensity of the color was measured at 450 nm.Standard curve was obmined by quantification of sHLA-Ⅰ standard reagents in series dilution.the levels of sHLA-Ⅰ antigens was examined in normal persons and RA patients.Results ①the sHLA-Ⅰ antigen concentration was(390±106)ng/ml in normal controls,(739±144)ng/ml in patients with active RA,and(524±114)ng/ml in patients with inactive RA.The sHLA-Ⅰ antigens concentrations in patients with active RA or inactive RA were significantly higher than those in normal controls (P＜0.01).especially the sHLA-Ⅰ antigen concentration in patients with active RA was significantly higher than that in patients with inaetive RA(P＜0.01).② The sHLA-Ⅰ antigens concentration in active RA patients Was significantly hisher than that in patients after treatment(744±148)ng/ml vs(533±106)ng/ml.P＜0.01).Conclusion The sHLA-Ⅰ antigen concentration in patients with RA is higher than that in normal controls,and increases more in active RA patients.