中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2012年
10期
688-691
,共4页
孙利平%武永明%杨丽丽%孟力平
孫利平%武永明%楊麗麗%孟力平
손리평%무영명%양려려%맹력평
红斑狼疮,系统性%高迁移率族蛋白1%Toll样受体%流式细胞术
紅斑狼瘡,繫統性%高遷移率族蛋白1%Toll樣受體%流式細胞術
홍반랑창,계통성%고천이솔족단백1%Toll양수체%류식세포술
Lupus erythematosus,systemic%High mobility group protein 1%TLRs%Flow cytometry
目的 通过检测系统性红斑狼疮(SLE)患者血清中高迁移率族蛋白1(HMGB1)及其外周血单核细胞上Toll样受体2(TLR2)的表达,评价其在SLE病情进展中的作用.方法 随机选取SLE患者40例,活动期与稳定期各20例,健康对照组20名,用酶联免疫吸附试验(ELISA)检测血清中HMGB1蛋白的表达,用流式细胞术分析外周血单核细胞上TLR2的表达,同时检测外周血补体C4、抗双链DNA(dsDNA)抗体、抗C1q抗体、抗核小体抗体,采用单因素方差分析和Kruskal-Wallis检验进行统计分析.结果 3组血清HMGB1分别为:活动组HMGB-1 [(48.9±11.3) μg/L],稳定组[(14.8±1.9)μg/L],对照组(13.5±3.6)μg/L],其中活动组血清HMGB-1表达水平较稳定组和对照组明显增高(P＜0.05);外周血单核细胞TLR2表达率活动组[(96.7±1.3)％],稳定组[(83.5±9.1)％],对照组[(83.3±9.9)％],其中活动组SLE患者TLR2的表达较稳定组SLE和对照组相比明显增高(P＜0.05);血清中HMGB1的表达水平与单核细胞表面TLR2的表达呈正相关(r=0.551,P＜0.05).结论 血清HMGB1和外周血单核细胞表TLR2均参与了SLE的病情发展过程.
目的 通過檢測繫統性紅斑狼瘡(SLE)患者血清中高遷移率族蛋白1(HMGB1)及其外週血單覈細胞上Toll樣受體2(TLR2)的錶達,評價其在SLE病情進展中的作用.方法 隨機選取SLE患者40例,活動期與穩定期各20例,健康對照組20名,用酶聯免疫吸附試驗(ELISA)檢測血清中HMGB1蛋白的錶達,用流式細胞術分析外週血單覈細胞上TLR2的錶達,同時檢測外週血補體C4、抗雙鏈DNA(dsDNA)抗體、抗C1q抗體、抗覈小體抗體,採用單因素方差分析和Kruskal-Wallis檢驗進行統計分析.結果 3組血清HMGB1分彆為:活動組HMGB-1 [(48.9±11.3) μg/L],穩定組[(14.8±1.9)μg/L],對照組(13.5±3.6)μg/L],其中活動組血清HMGB-1錶達水平較穩定組和對照組明顯增高(P＜0.05);外週血單覈細胞TLR2錶達率活動組[(96.7±1.3)％],穩定組[(83.5±9.1)％],對照組[(83.3±9.9)％],其中活動組SLE患者TLR2的錶達較穩定組SLE和對照組相比明顯增高(P＜0.05);血清中HMGB1的錶達水平與單覈細胞錶麵TLR2的錶達呈正相關(r=0.551,P＜0.05).結論 血清HMGB1和外週血單覈細胞錶TLR2均參與瞭SLE的病情髮展過程.
목적 통과검측계통성홍반랑창(SLE)환자혈청중고천이솔족단백1(HMGB1)급기외주혈단핵세포상Toll양수체2(TLR2)적표체,평개기재SLE병정진전중적작용.방법 수궤선취SLE환자40례,활동기여은정기각20례,건강대조조20명,용매련면역흡부시험(ELISA)검측혈청중HMGB1단백적표체,용류식세포술분석외주혈단핵세포상TLR2적표체,동시검측외주혈보체C4、항쌍련DNA(dsDNA)항체、항C1q항체、항핵소체항체,채용단인소방차분석화Kruskal-Wallis검험진행통계분석.결과 3조혈청HMGB1분별위:활동조HMGB-1 [(48.9±11.3) μg/L],은정조[(14.8±1.9)μg/L],대조조(13.5±3.6)μg/L],기중활동조혈청HMGB-1표체수평교은정조화대조조명현증고(P＜0.05);외주혈단핵세포TLR2표체솔활동조[(96.7±1.3)％],은정조[(83.5±9.1)％],대조조[(83.3±9.9)％],기중활동조SLE환자TLR2적표체교은정조SLE화대조조상비명현증고(P＜0.05);혈청중HMGB1적표체수평여단핵세포표면TLR2적표체정정상관(r=0.551,P＜0.05).결론 혈청HMGB1화외주혈단핵세포표TLR2균삼여료SLE적병정발전과정.
Objective To evaluate the pathogenesis and disease progression by detecting the expression of Serum High mobility group protein 1 (HMGB1) and TLR2 in monocytes of patients with systemic lupus erythematosus (SLE).Methods Forty patients with SLE were selected randomly,20 patients were in active disease group and others were in stable disease group.The expression of HMGB1 in the serum of these cases were detected by enzyme linked immunosorbent assay (ELISA) and TLR2 on CD14+ monocytes in the peripheral blood were detected by FCM.The correlation between these indexes and clinical,laboratory indexes about SLE were analyzed using one-way ANOVA,and Kruskal-Wallis test.Results The expression levels of HMGB-1 in serum was [(48.9±11.3) μg/L] in the active group,while that was [(14.8±1.9) μg/L] in the stable group was,and [(13.5±3.6) μg/L] in the control group.HMGB1 in the active SLE group was significantly higher (P＜0.05) when compared with that of the stable and control group.The expression of TLR2 in the peripheral blood mononuclear cells was [(96.7±1.3)％] in the active group,[(83.5±9.1)％] in the stable group,and [(83.3±9.9)％] in the control group TLR2 in the active SLE group was up-regulated when compared with the stable and control groups (P＞0.05).There were positive correlation between the serum levels of HMGB1and TLR2 in the peripheral blood mononuclear cells (r=0.551,P＜0.05).Conclusion The expression levels of HMGB-1 in serum and the expression of TLR2 in peripheral blood mononuclear cells may participate in the pathological processes of SLE.
