CAJ | 학술논문

目的在蛋白和mRNA水平上研究结构型热休克蛋白70(Hsc70)在类风湿关节炎(RA)患者滑膜和血液中的表达水平,探讨该蛋白对RA的病理作用.方法用免疫组织化学法、实时荧光定量聚合酶链反应、蛋白印迹法分析Hsc70在RA滑膜中的表达水平；以健康人为对照,用酶联免疫吸附法分析Hsc70在RA患者血液中的表达水平.采用单因素方差分析、LSD检验、Spearman相关分析进行统计学处理.结果与骨关节炎和强直性脊柱炎比较,Hsc70在RA滑膜高表达,表达率为100％.Hsc70在RA血液中表达量(吸光度值0.32±0.09)也高于健康组(吸光度值0.16±0.03)的表达量(P＜0.01).结论 Hsc70在RA滑膜组织中高表达,提示Hsc70在RA发病过程中可能起重要作用.
목적 재단백화mRNA수평상연구결구형열휴극단백70(Hsc70)재류풍습관절염(RA)환자활막화혈액중적표체수평,탐토해단백대RA적병리작용.방법 용면역조직화학법、실시형광정량취합매련반응、단백인적법분석Hsc70재RA활막중적표체수평；이건강인위대조,용매련면역흡부법분석Hsc70재RA환자혈액중적표체수평.채용단인소방차분석、LSD검험、Spearman상관분석진행통계학처리.결과 여골관절염화강직성척주염비교,Hsc70재RA활막고표체,표체솔위100％.Hsc70재RA혈액중표체량(흡광도치0.32±0.09)야고우건강조(흡광도치0.16±0.03)적표체량(P＜0.01).결론 Hsc70재RA활막조직중고표체,제시Hsc70재RA발병과정중가능기중요작용.
Objective The present study investigated the expression of heat shock cognate protein 70 (Hsc70) in the synovial tissues and blood samples of patients with rheumatoid arthritis (RA) to determine the pathological role of this protein in the pathogenesis of the disease.Methods The expression of Hsc70 in synovial membranes was quantitatively analyzed by immunohistochemistry,real-time quantitative PCR and western blotting.The samples from osteoarthritis (OA) and ankylosing spondylitis (AS) were used as controls.The levels of Hsc70 in blood of patients with RA were determined using enzyme linked immunosorbent assay (ELISA) with the samples of the healthy subjects as controls.Statistical analysis was conducted with one-way ANOVA,LSD test and Spearmen's correlation.Results Immunohistochemistry showed that Hsc70 had significantly increased expression in synovial tissues of RA than in the samples of OA and AS.Real-time PCR and western blotting confirmed the above findings.ELISA detected significantly elevated level of Hsc70 in blood of patients with RA as compared with samples from the controls (P＜0.01).Conclusion The study suggests that the up-regulation of Hsc70 may be involved in the pathogenic process of RA.