中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2013年
1期
20-22
,共3页
贾汝琳%赵静%孙晓麟%孙凌云%粟占国%李霞
賈汝琳%趙靜%孫曉麟%孫凌雲%粟佔國%李霞
가여림%조정%손효린%손릉운%속점국%리하
关节炎,类风湿%受体,肿瘤坏死因子,成员6b%类风湿因子%抗环瓜氨酸肽抗体
關節炎,類風濕%受體,腫瘤壞死因子,成員6b%類風濕因子%抗環瓜氨痠肽抗體
관절염,류풍습%수체,종류배사인자,성원6b%류풍습인자%항배과안산태항체
Arthritis,rheumatoid%Receptors,tumor necrosis factor,member 6b%Rheumatoid factor%Anti-cyclic citrullinated peptide antibodies
目的 检测类风湿关节炎(RA)患者血清中诱骗受体3(DcR3)的水平,分析其在RA中的临床意义.方法 收集RA患者血清180例,健康对照者100名,骨关节炎患者29例,系统性红斑狼疮(SLE)患者60例,干燥综合征(SS)患者30例,采用双抗体夹心酶联免疫吸附试验(ELISA)测定血清中的DcR3水平,并分析血清DcR3水平与RA各临床和实验室指标的相关性,计量资料的比较采用独立样本t检验,计数资料比较采用x2检验,相关性分析采用Spearman相关分析.结果 RA患者血清DcR3水平为(202±261) ng/ml,显著高于健康对照组[(32±31) ng/ml,t=5.33,P＜0.01],OA患者[(90±33) ng/ml,t=1.998,P＜0.01],SLE患者[(41±56) ng/ml,t=3.19,P＜0.01]和SS患者[(25±31) ng/ml,t=2.50,P＜0.01]；根据健康人和其他疾病对照确定DcR3的诊断浓度为45.86 ng/ml,敏感性为0.991,特异性为0.765.血清DcR3水平与类风湿因子(RF) IgM、RF-IgG、抗环瓜氨酸肽(CCP)抗体呈正相关(r值分别为0.58,0.54,0.411,P＜0.05),与补体C3 (C3)呈负相关(r=-0.226,P＜0.05).结论 DcR3在RA患者血清中表达增高,并与疾病免疫指标密切相关(包括RF-IgM、RF-IgG、C3和抗CCP抗体),可能是RA预后不良因素之一.
目的 檢測類風濕關節炎(RA)患者血清中誘騙受體3(DcR3)的水平,分析其在RA中的臨床意義.方法 收集RA患者血清180例,健康對照者100名,骨關節炎患者29例,繫統性紅斑狼瘡(SLE)患者60例,榦燥綜閤徵(SS)患者30例,採用雙抗體夾心酶聯免疫吸附試驗(ELISA)測定血清中的DcR3水平,併分析血清DcR3水平與RA各臨床和實驗室指標的相關性,計量資料的比較採用獨立樣本t檢驗,計數資料比較採用x2檢驗,相關性分析採用Spearman相關分析.結果 RA患者血清DcR3水平為(202±261) ng/ml,顯著高于健康對照組[(32±31) ng/ml,t=5.33,P＜0.01],OA患者[(90±33) ng/ml,t=1.998,P＜0.01],SLE患者[(41±56) ng/ml,t=3.19,P＜0.01]和SS患者[(25±31) ng/ml,t=2.50,P＜0.01]；根據健康人和其他疾病對照確定DcR3的診斷濃度為45.86 ng/ml,敏感性為0.991,特異性為0.765.血清DcR3水平與類風濕因子(RF) IgM、RF-IgG、抗環瓜氨痠肽(CCP)抗體呈正相關(r值分彆為0.58,0.54,0.411,P＜0.05),與補體C3 (C3)呈負相關(r=-0.226,P＜0.05).結論 DcR3在RA患者血清中錶達增高,併與疾病免疫指標密切相關(包括RF-IgM、RF-IgG、C3和抗CCP抗體),可能是RA預後不良因素之一.
목적 검측류풍습관절염(RA)환자혈청중유편수체3(DcR3)적수평,분석기재RA중적림상의의.방법 수집RA환자혈청180례,건강대조자100명,골관절염환자29례,계통성홍반랑창(SLE)환자60례,간조종합정(SS)환자30례,채용쌍항체협심매련면역흡부시험(ELISA)측정혈청중적DcR3수평,병분석혈청DcR3수평여RA각림상화실험실지표적상관성,계량자료적비교채용독립양본t검험,계수자료비교채용x2검험,상관성분석채용Spearman상관분석.결과 RA환자혈청DcR3수평위(202±261) ng/ml,현저고우건강대조조[(32±31) ng/ml,t=5.33,P＜0.01],OA환자[(90±33) ng/ml,t=1.998,P＜0.01],SLE환자[(41±56) ng/ml,t=3.19,P＜0.01]화SS환자[(25±31) ng/ml,t=2.50,P＜0.01]；근거건강인화기타질병대조학정DcR3적진단농도위45.86 ng/ml,민감성위0.991,특이성위0.765.혈청DcR3수평여류풍습인자(RF) IgM、RF-IgG、항배과안산태(CCP)항체정정상관(r치분별위0.58,0.54,0.411,P＜0.05),여보체C3 (C3)정부상관(r=-0.226,P＜0.05).결론 DcR3재RA환자혈청중표체증고,병여질병면역지표밀절상관(포괄RF-IgM、RF-IgG、C3화항CCP항체),가능시RA예후불량인소지일.
Objective To test serum decoy receptor 3 (DcR3) concentration in patients with rheumatoid arthritis (RA),and analyze its clinical significance.Methods The serum from 180 RA patients,29 osteoarthritis (OA) patients,60 systemic lupus erythematosus (SLE) patients,30 Sj(o)gren's syndrome (SS) patients and 100 healthy donators were selected.The serum DcR3 concentrations of the two groups were detected by enzyme-linked immunosorbent assay (ELISA).The correlation between serum DcR3 levels and clinical features of patients with RA were investigated.T test,x2 test and Spearman's correlation analysis were used for statistical analysis with software SPSS 16.0.Results Serum DcR3 levels in RA patients [(202±261)ng/ml] were significantly higher than those of the healthy controls [(32±31) ng/ml,t=5.33,P＜0.01],OA patients [(90±33)ng/ml,t=1.998,P＜0.01],SLE patients [(41±56)ng/ml,t=3.19,P＜0.01],and SS patients [(25±31) ng/ml,t=2.50,P＜0.01].The DcR3 cut value was 45.86 ng/ml for the diagnoses of RA,the sensitivity and specificity was 0.991 and 0.765 respectively.The levels of serum DcR3 in RA patients were related with rheumatoid factor (RF) IgM,RF-IgG,anti-cyclic citrullinated peptide antibodies (anti-CCP) and C3.Conclusion Serum DcR3 level is significantly elevated in RA and it is significantly associated with immunological index of RA including RF-IgM,RF-IgG,C3 and anti-CCP antibody,which may be a factor for unfavorable prognosis of RA patients.
