CAJ | 학술논문

目的探讨系统性红斑狼疮(SLE)患者外周血骨形态发生蛋白(BMP)通路相关微小RNA(miR)-21、-155的表达水平及其临床意义.方法收集59例SLE患者和25名健康对照外周血,分离外周血单个核细胞(PBMC),Trizol法提取总RNA,逆转录过程中加poly A尾,以实时荧光定量聚合酶链反应(PCR)技术进行检测.以SLE疾病活动指数(SLEDAI)进行疾病活动性评分,根据临床症状或实验室检查结果将SLE患者分组,不同组间行Mann-Whitney秩和检验.结果与健康对照组相比,中重度活动组(SLEDAI≥8)SLE患者miR-21、-155表达水平均升高(P＜0.05),而轻度活动组(SLEDAI≤7)与健康对照组差异无统计学意义(P＞0.05)；抗双链DNA(dsDNA)(+) SLE患者miR-21、-155表达水平显著升高(P＜0.05)；与无狼疮肾炎组相比,狼疮肾炎组SLE患者miR-21、-155表达水平升高(P＜0.05)；而miRNA表达水平与其余临床及实验室指标(血液系统异常、浆膜炎及脉管炎等)未见相关(P＞0.05).结论中重度活动SLE患者miR-21、-155表达升高,且与抗dsDNA抗体及肾脏损伤相关,提示miR-21和-155表达异常可能在SLE疾病活动中起作用.
목적 탐토계통성홍반랑창(SLE)환자외주혈골형태발생단백(BMP)통로상관미소RNA(miR)-21、-155적표체수평급기림상의의.방법 수집59례SLE환자화25명건강대조외주혈,분리외주혈단개핵세포(PBMC),Trizol법제취총RNA,역전록과정중가poly A미,이실시형광정량취합매련반응(PCR)기술진행검측.이SLE질병활동지수(SLEDAI)진행질병활동성평분,근거림상증상혹실험실검사결과장SLE환자분조,불동조간행Mann-Whitney질화검험.결과 여건강대조조상비,중중도활동조(SLEDAI≥8)SLE환자miR-21、-155표체수평균승고(P＜0.05),이경도활동조(SLEDAI≤7)여건강대조조차이무통계학의의(P＞0.05)；항쌍련DNA(dsDNA)(+) SLE환자miR-21、-155표체수평현저승고(P＜0.05)；여무랑창신염조상비,랑창신염조SLE환자miR-21、-155표체수평승고(P＜0.05)；이miRNA표체수평여기여림상급실험실지표(혈액계통이상、장막염급맥관염등)미견상관(P＞0.05).결론 중중도활동SLE환자miR-21、-155표체승고,차여항dsDNA항체급신장손상상관,제시miR-21화-155표체이상가능재SLE질병활동중기작용.
Objective To study the expression of 2 bone morphogenetic protein (BMP) related microRNA (miR-21,miR-155) in SLE patients,and to analyze their correlation with clinical features.Methods Peripheral blood was obtained from 59 SLE patients and 25 healthy controls,and total RNA was extracted and reverse transcribed into complementary DNA (cDNA).Real-time PCR technique was used to detect gene expression at transcrptional level.Disease activity was determined by SLEDAI score.Patients were divided into different groups based on their manifestations or antibody profiles,then comparison of miRNA expression was carried out using Mann-Whitney test.Results The expression levels of miR-21 and miR-155 were increased in active SLE patients (SLEDAI≥8) as compared to those with mild disease (SLEDAI≤7) or healthy controls (both P＜0.05).Patients with positive anti-dsDNA or lupus nephritis had higher expression levels of miR-21 and miR-155 than those without (P＜0.05).Conclusion The miR-21 and miR-155 expressions are elevated in active SLE patients and are associated with anti-dsDNA and renal involvement.The results suggest that these two miRNAs might play a role in the pathogenesis of SLE disease.