CAJ | 학술논문

目的探讨嘌呤受体P2X配体门控性离子通道7(P2X7R)在初发系统性红斑狼疮(SLE)患者外周血淋巴细胞上的表达及其与部分炎症细胞因子的相关性.方法选择29例初发SLE患者及28名健康对照,分离外周血单个核细胞(PBMC),采用流式细胞术检测淋巴细胞、CD4+淋巴细胞、CD19+淋巴细胞上P2X7R的表达水平,酶联免疫吸附试验(ELISA)检测P2X7R相关的细胞因子白细胞介素(IL)-1β、IL-6及肿瘤坏死因子(TNF)-α水平.采用t检验、Wilcoxon秩和检验和Sperman相关分析进行统计学分析.结果 ①SLE患者外周血CD4+淋巴细胞、CD19+淋巴细胞表面P2X7R的表达明显高于健康对照组[CD4+淋巴细胞:2.21 (3.55)和0.89 (1.15),Z=-1.527,P=0.015； CD19+淋巴细胞:11.53 (20.01)和6.66(6.27),Z=-2.091,P=0.037]；②SLE患者血清中3种细胞因子水平均明显高于健康对照组,差异有统计学意义；SLE患者外周血淋巴细胞中P2X7R表达水平与IL-6呈正相关(r=0.449,P=0.015)；③SLE患者中,关节炎组淋巴细胞上P2X7R表达显著高于非关节炎组[3.84(11.53)与0.90(1.81),Z=-2.772,P=-0.006]；P2X7R在淋巴细胞及CD19+淋巴细胞中的表达均与SLE疾病活动指数(SLEDAI)评分呈正相关；淋巴细胞上的P2X7R表达与抗β2糖蛋白Ⅰ抗体呈正相关(r=0.575,P=0.008).结论 P2X7R可能通过介导炎症因子的释放参与SLE发病；可能与SLE患者的关节炎、狼疮肾炎及神经精神狼疮相关.
목적 탐토표령수체P2X배체문공성리자통도7(P2X7R)재초발계통성홍반랑창(SLE)환자외주혈림파세포상적표체급기여부분염증세포인자적상관성.방법 선택29례초발SLE환자급28명건강대조,분리외주혈단개핵세포(PBMC),채용류식세포술검측림파세포、CD4+림파세포、CD19+림파세포상P2X7R적표체수평,매련면역흡부시험(ELISA)검측P2X7R상관적세포인자백세포개소(IL)-1β、IL-6급종류배사인자(TNF)-α수평.채용t검험、Wilcoxon질화검험화Sperman상관분석진행통계학분석.결과 ①SLE환자외주혈CD4+림파세포、CD19+림파세포표면P2X7R적표체명현고우건강대조조[CD4+림파세포:2.21 (3.55)화0.89 (1.15),Z=-1.527,P=0.015； CD19+림파세포:11.53 (20.01)화6.66(6.27),Z=-2.091,P=0.037]；②SLE환자혈청중3충세포인자수평균명현고우건강대조조,차이유통계학의의；SLE환자외주혈림파세포중P2X7R표체수평여IL-6정정상관(r=0.449,P=0.015)；③SLE환자중,관절염조림파세포상P2X7R표체현저고우비관절염조[3.84(11.53)여0.90(1.81),Z=-2.772,P=-0.006]；P2X7R재림파세포급CD19+림파세포중적표체균여SLE질병활동지수(SLEDAI)평분정정상관；림파세포상적P2X7R표체여항β2당단백Ⅰ항체정정상관(r=0.575,P=0.008).결론 P2X7R가능통과개도염증인자적석방삼여SLE발병；가능여SLE환자적관절염、랑창신염급신경정신랑창상관.
Objective To analyze the expression of purinergic receptor P2X ligand-gated ion channel 7 (P2X7R) on different cells and peripheral blood mononuclear cell (PBMC) and to investigate its correlation with inflammatory cytokines in patients with SLE.Methods Flow cytometry was used to detect surface expression of P2X7R on lymphocytes,CD4+ cells,and CD19+ cell in 29 SLE patients and 28 healthy human controls to compare the difference between the SLE patients and the controls in P2X7R expression.Enzyme linked immunosorbent assay (ELISA) was performed to detect P2X7R-related serum cytokines interleukin (IL)-1β,IL-6,tumor necrosis factor (TNF)-α level.T test,Wilcoxon rank sum test,Spearman's correlation analysis were used for statitical analysis.Results ① SLE patients had significantly higher expression of P2X7R on CD4+,CD8+ lymphocytes compared to controls [CD4+ cells∶ 2.21(3.55) vs 0.89(1.15),Z=-1.527,P=0.015； CD19+ cells∶ 11.53(20.01) vs 6.66 (6.27),Z=-2.091,P=0.037]； ② The levels of three cytokines in patients with SLE were significantly higher than those in control.The positive relationship between P2X7R expression in lymphocytes with the serum IL-6 level was found in SLE patients (r=0.449,P=0.015)；③ Patients with arthritis showed significantly higher expression of P2X7R on lym-phocytes compared to patients without arthritis (Z=-2.772,P=0.006).The expression of P2X7R on lymphocytes and CD19+ cell was significantly positively correlated with the SLEDAI score.Positive correlation with anti-β2GP Ⅰ in lymphocyteswas also found.Conclusion P2X7R may mediate the release of inflammatory cytokines involved in the pathogenesis of SLE,and may participate the development of arthritis,lupus nephritis and NPSLE in SLE patients.