中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2013年
2期
98-102
,共5页
刘洪彦%郑毅%史旭华%陈珊珊
劉洪彥%鄭毅%史旭華%陳珊珊
류홍언%정의%사욱화%진산산
关节炎,类风湿%白细胞介素18%白细胞介素33%半胱氨酸天冬氨酸蛋白酶1
關節炎,類風濕%白細胞介素18%白細胞介素33%半胱氨痠天鼕氨痠蛋白酶1
관절염,류풍습%백세포개소18%백세포개소33%반광안산천동안산단백매1
Arthritis,rheumatoid%Interleukin-18%Interleukin-33%Caspase-1
目的 检测类风湿关节炎(RA)患者血清半胱氨酸蛋白酶-1(caspase-1)及其下游因子白细胞介素(IL)-18、IL-33水平,并探讨三者在RA中的作用.方法 收集56例RA患者及22名健康体检者血清及临床资料,应用酶联免疫吸附试验(ELISA)法检测caspase-1、IL-18及IL-33水平.采用Kruskal-Walls及Mann-Whitney U检验方法对血清caspase-1,IL-18和IL-33进行比较,并通过Spearman方法进行相关性分析.结果 RA患者caspase-1水平明显高于健康人群[(32±26) ng/ml与(15±6) ng/mL,P＜0.01],且活动组高于缓解组,未治疗者高于接受治疗者[(47±27)与(25±22) ng/mL,P＜0.01].RA患者IL-18和IL-33水平亦明显高于健康人群[分别为(121±121)与(58±33) ng/L,(1032±1011) ng/L与(510±231) ng/L,P＜0.05],且活动组高于缓解组,未治疗者高于接受治疗者[分别为(172±139) ng/L与(97±106) ng/L,(1468±1039) ng/L与(825±941) ng/L,P＜0.01].caspase-1与IL-18,IL-33呈正相关(r分别为0.824,0.854,P均＜0.01).IL-18与IL-33呈正相关(r=0.800,P＜0.01).caspase-1,IL-18及IL-33与病程、类风湿因子(RF)、抗环瓜氨酸肽(CCP)抗体、压痛关节数及肿胀关节数无相关性.结论 caspase-1及其下游因子IL-18、IL-33在RA患者中升高,且前者与后两者密切相关,caspase-1可能在RA中发挥重要作用.
目的 檢測類風濕關節炎(RA)患者血清半胱氨痠蛋白酶-1(caspase-1)及其下遊因子白細胞介素(IL)-18、IL-33水平,併探討三者在RA中的作用.方法 收集56例RA患者及22名健康體檢者血清及臨床資料,應用酶聯免疫吸附試驗(ELISA)法檢測caspase-1、IL-18及IL-33水平.採用Kruskal-Walls及Mann-Whitney U檢驗方法對血清caspase-1,IL-18和IL-33進行比較,併通過Spearman方法進行相關性分析.結果 RA患者caspase-1水平明顯高于健康人群[(32±26) ng/ml與(15±6) ng/mL,P＜0.01],且活動組高于緩解組,未治療者高于接受治療者[(47±27)與(25±22) ng/mL,P＜0.01].RA患者IL-18和IL-33水平亦明顯高于健康人群[分彆為(121±121)與(58±33) ng/L,(1032±1011) ng/L與(510±231) ng/L,P＜0.05],且活動組高于緩解組,未治療者高于接受治療者[分彆為(172±139) ng/L與(97±106) ng/L,(1468±1039) ng/L與(825±941) ng/L,P＜0.01].caspase-1與IL-18,IL-33呈正相關(r分彆為0.824,0.854,P均＜0.01).IL-18與IL-33呈正相關(r=0.800,P＜0.01).caspase-1,IL-18及IL-33與病程、類風濕因子(RF)、抗環瓜氨痠肽(CCP)抗體、壓痛關節數及腫脹關節數無相關性.結論 caspase-1及其下遊因子IL-18、IL-33在RA患者中升高,且前者與後兩者密切相關,caspase-1可能在RA中髮揮重要作用.
목적 검측류풍습관절염(RA)환자혈청반광안산단백매-1(caspase-1)급기하유인자백세포개소(IL)-18、IL-33수평,병탐토삼자재RA중적작용.방법 수집56례RA환자급22명건강체검자혈청급림상자료,응용매련면역흡부시험(ELISA)법검측caspase-1、IL-18급IL-33수평.채용Kruskal-Walls급Mann-Whitney U검험방법대혈청caspase-1,IL-18화IL-33진행비교,병통과Spearman방법진행상관성분석.결과 RA환자caspase-1수평명현고우건강인군[(32±26) ng/ml여(15±6) ng/mL,P＜0.01],차활동조고우완해조,미치료자고우접수치료자[(47±27)여(25±22) ng/mL,P＜0.01].RA환자IL-18화IL-33수평역명현고우건강인군[분별위(121±121)여(58±33) ng/L,(1032±1011) ng/L여(510±231) ng/L,P＜0.05],차활동조고우완해조,미치료자고우접수치료자[분별위(172±139) ng/L여(97±106) ng/L,(1468±1039) ng/L여(825±941) ng/L,P＜0.01].caspase-1여IL-18,IL-33정정상관(r분별위0.824,0.854,P균＜0.01).IL-18여IL-33정정상관(r=0.800,P＜0.01).caspase-1,IL-18급IL-33여병정、류풍습인자(RF)、항배과안산태(CCP)항체、압통관절수급종창관절수무상관성.결론 caspase-1급기하유인자IL-18、IL-33재RA환자중승고,차전자여후량자밀절상관,caspase-1가능재RA중발휘중요작용.
Objective To measure the levels of caspase-1 and its downstream factor interleukin (IL)-18 and IL-33 in rheumatoid arthritis (RA) and explore their possible mechanisms.Methods Blood samples were drown from 56 patients with RA and 22 healthy subjects.Serum levels of caspase-1,IL-18 and IL-33were tested by the method of enzyme linked immunosorbent assay (ELISA).Kruskal-Walls and Mann-Whitney test were used to compare the levels of caspase-1,[L-18 and IL-33 and Spearman's correlation test was used for correlation analysis.Results The level of caspase-1 was significantly increased in RA group compared to healthy group [(32±26) ng/ml vs (15±6) ng/ml,P＜0.01].Meanwhile,the active disease groups showed a higher level than the remission group,and level in the untreated group was higher than the treated group [(47±27) ng/ml vs (25±22) ng/ml,P＜0.01].The levels of IL-18 and IL-33 were significantly increased in RA group compared to healthy group [(121±121) ng/L vs (58±33) ng/L,(1032±1011) ng/L vs (510±231)ng/L,respectively,P＜0.05].Meanwhile,the active disease groups had a higher level than the remission group and the untreated group had higher levels than the treated group [IL-18 and IL-33 were (172±139) ng/L vs (97±106) ng/L,(1469±1039) ng/L vs (825±941) ng/L,respectively,P＜0.05].Caspase-1 was correlated withIL-18 and IL-33 (r=0.824,0.854,P＜0.01) and IL-18 was correlated with IL-33 (r=0.800,P＜0.01).But neither of the three factors was related with clinical indexes including disease duration,RF,anti-CCP antibody,tender joints count and swollen joints count.Conclusion Caspase-1 and its downstream factor IL-18,IL-33 increase in RA,and they may play important roles in RA.
