中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2013年
2期
127-129
,共3页
霍玉萍%闫乎玲%耿松梅%胡倩婷%张志扬
霍玉萍%閆乎玲%耿鬆梅%鬍倩婷%張誌颺
곽옥평%염호령%경송매%호천정%장지양
皮肌炎%T淋巴细胞,辅助诱导%Th17细胞
皮肌炎%T淋巴細胞,輔助誘導%Th17細胞
피기염%T림파세포,보조유도%Th17세포
Dermatomyositis%T-lymphocytes,helper-inducer%Th17 cell
目的 探讨皮肌炎患者外周血调节性T细胞/辅助性T细胞17(Th17)的变化及意义.方法 选取36例皮肌炎患者和36名健康对照者,分别采用流式细胞术、实时定量逆转录聚合酶链反应和酶联免疫吸附法法检测外周血调节性T细胞/Th17细胞百分率、核转录因子叉头翼状螺旋转录因子3(Foxp3)/视黄醇类核内受体(γ)t(RORγt) mRNA的表达以及血浆转化生长因子(TGF)-β1、白细胞介素(IL)-17的水平.统计学处理采用t检验,相关分析采用Pearson直线相关分析.结果 皮肌炎患者CD4+Foxp3+调节性T细胞百分率[(7.2±2.3)％]、Foxp3 mRNA表达水平(0.44±0.19)以及血浆TGF-β1水平[(37400±4814) pg/ml]与健康对照组相比显著降低[(9.1±3.2)％、0.62±0.25、(41 200±6348) pg/ml,P均＜0.05]；与对照组相比,皮肌炎患者外周血中Th17细胞百分率[(1.22±0.41)％]、RORγt mRNA表达水平(0.85±0.34)及血浆Ⅱ-17水平[:(77±32) pg/ml]显著高于对照组[(0.83±0.14)％、0.61±0.21、(58±20) pg/ml] (P均＜0.05).CD4+Foxp3调节性T细胞百分率、Foxp3 mRNA表达水平及血浆TGF-β1水平与血清肌酸激酶水平呈负相关(r=-0.573,-0.593,-0.618,P＜0.05),Th17细胞百分率、RORγt mRNA表达水平及血浆IL-17水平与肌酸激酶水平呈正相关(r=0.582,0.609,0,623,P＜0.05).结论 皮肌炎患者外周血中调节性T细胞/Th17细胞失衡可能与皮肌炎的发病有关.
目的 探討皮肌炎患者外週血調節性T細胞/輔助性T細胞17(Th17)的變化及意義.方法 選取36例皮肌炎患者和36名健康對照者,分彆採用流式細胞術、實時定量逆轉錄聚閤酶鏈反應和酶聯免疫吸附法法檢測外週血調節性T細胞/Th17細胞百分率、覈轉錄因子扠頭翼狀螺鏇轉錄因子3(Foxp3)/視黃醇類覈內受體(γ)t(RORγt) mRNA的錶達以及血漿轉化生長因子(TGF)-β1、白細胞介素(IL)-17的水平.統計學處理採用t檢驗,相關分析採用Pearson直線相關分析.結果 皮肌炎患者CD4+Foxp3+調節性T細胞百分率[(7.2±2.3)％]、Foxp3 mRNA錶達水平(0.44±0.19)以及血漿TGF-β1水平[(37400±4814) pg/ml]與健康對照組相比顯著降低[(9.1±3.2)％、0.62±0.25、(41 200±6348) pg/ml,P均＜0.05]；與對照組相比,皮肌炎患者外週血中Th17細胞百分率[(1.22±0.41)％]、RORγt mRNA錶達水平(0.85±0.34)及血漿Ⅱ-17水平[:(77±32) pg/ml]顯著高于對照組[(0.83±0.14)％、0.61±0.21、(58±20) pg/ml] (P均＜0.05).CD4+Foxp3調節性T細胞百分率、Foxp3 mRNA錶達水平及血漿TGF-β1水平與血清肌痠激酶水平呈負相關(r=-0.573,-0.593,-0.618,P＜0.05),Th17細胞百分率、RORγt mRNA錶達水平及血漿IL-17水平與肌痠激酶水平呈正相關(r=0.582,0.609,0,623,P＜0.05).結論 皮肌炎患者外週血中調節性T細胞/Th17細胞失衡可能與皮肌炎的髮病有關.
목적 탐토피기염환자외주혈조절성T세포/보조성T세포17(Th17)적변화급의의.방법 선취36례피기염환자화36명건강대조자,분별채용류식세포술、실시정량역전록취합매련반응화매련면역흡부법법검측외주혈조절성T세포/Th17세포백분솔、핵전록인자차두익상라선전록인자3(Foxp3)/시황순류핵내수체(γ)t(RORγt) mRNA적표체이급혈장전화생장인자(TGF)-β1、백세포개소(IL)-17적수평.통계학처리채용t검험,상관분석채용Pearson직선상관분석.결과 피기염환자CD4+Foxp3+조절성T세포백분솔[(7.2±2.3)％]、Foxp3 mRNA표체수평(0.44±0.19)이급혈장TGF-β1수평[(37400±4814) pg/ml]여건강대조조상비현저강저[(9.1±3.2)％、0.62±0.25、(41 200±6348) pg/ml,P균＜0.05]；여대조조상비,피기염환자외주혈중Th17세포백분솔[(1.22±0.41)％]、RORγt mRNA표체수평(0.85±0.34)급혈장Ⅱ-17수평[:(77±32) pg/ml]현저고우대조조[(0.83±0.14)％、0.61±0.21、(58±20) pg/ml] (P균＜0.05).CD4+Foxp3조절성T세포백분솔、Foxp3 mRNA표체수평급혈장TGF-β1수평여혈청기산격매수평정부상관(r=-0.573,-0.593,-0.618,P＜0.05),Th17세포백분솔、RORγt mRNA표체수평급혈장IL-17수평여기산격매수평정정상관(r=0.582,0.609,0,623,P＜0.05).결론 피기염환자외주혈중조절성T세포/Th17세포실형가능여피기염적발병유관.
Objective To explore the changes and clinical significance of regulatory T cell/T help cell 17 (Th17) in the peripheral blood from patients with dermatomyositis (DM).Methods Thirty-six patients with DM were recruited and thirty-six healthy individuals were enrolled as normal controls.The percentage of regulatory T cell/Th17 cell was assessed by flow cytometry and the mRNA levels of Foxp3/RORγt were detected by real-time quantitative reverse wanscription polymerase chain reaction (RT-PCR).The plasma levels of TGF-β1/IL-17 were measured by enzyme linked immunosorbent assay (ELISA).T-test was used for statistical analysis and Pearson's linear analysis were used for correlation analysis.Results The percentage of CD4+ Foxp3+ T cell,the mRNA expression of Foxp3 and the plasma levels of TGF-β1 decreased significantly in patients with DM than in normal controls [(7.2±2.3)％ vs (9.1±3.2)％,(0.44±0.19) vs (0.62±0.25),(37 400±4814) pg/ml vs (41 200±6348) pg/ml,P＜0.05].The percentage of Th17 cell,the mRNA expression of RORγt and the plasma levels of IL-17 increased in patients with DM compared with normal controls [(1.22±0.41)％ vs (0.83±0.14)％,(0.85±0.34) vs (0.61±0.21),(77±32) pg/ml vs (58±20) pg/ml,P＜0.05].There was negative correlation between the percentage of regulatory T cell,the mRNA levels of Foxp3,the plasma levels of TGF-β1 and the serum levels of CK in patients with DM (r=-0.573,-0.593,-0.618,P＜0.05).There was positive correhtion between the percentage of Thl7 cell,the mRNA levels of RORγt,the plasma levels of IL-17 and the serum levels of CK in patients with DM (r=0.582,0.609,0.623,P＜0.05).Conclusion The imbalance of regulatory T cell/Th17 cell may exist in the peripheral blood of patients with DM.Regulatory T cell/Th17 cell may be associated with the pathology of DM.
