CAJ | 학술논문

目的观察强直性脊柱炎(AS)棘上韧带在脊柱骨性强直后发生的组织病理学及超微结构改变.方法通过光镜下观察苏木素-伊红(HE)、苦味酸-天狼星红染色,并行电镜观察微观结构改变,与对照组进行比较.图像处理软件分析血管密度、胶原原纤维分布密度.采用t检验进行统计学分析.结果实验组光镜下,胶原纤维排列紊乱,可见玻璃样变及脂肪浸润,大量血管增生,管径不一,有Ⅲ型胶原表达,电镜下胶原纤维分布稀疏,周期性横纹模糊,基质降解,可见基质小泡.对照组胶原纤维排列规则,无上述改变.实验组血管密度(6.8±0.7)/HP,对照组(1.0±0.4)/HP;实验组胶原原纤维分布密度(122±22)/μm2,对照组(218±10)/μm2,2组差异有统计学意义(P＜0.05).结论棘上韧带在AS疾病进展过程中出现一系列的组织病理学改变.
목적 관찰강직성척주염(AS)극상인대재척주골성강직후발생적조직병이학급초미결구개변.방법 통과광경하관찰소목소-이홍(HE)、고미산-천랑성홍염색,병행전경관찰미관결구개변,여대조조진행비교.도상처리연건분석혈관밀도、효원원섬유분포밀도.채용t검험진행통계학분석.결과 실험조광경하,효원섬유배렬문란,가견파리양변급지방침윤,대량혈관증생,관경불일,유Ⅲ형효원표체,전경하효원섬유분포희소,주기성횡문모호,기질강해,가견기질소포.대조조효원섬유배렬규칙,무상술개변.실험조혈관밀도(6.8±0.7)/HP,대조조(1.0±0.4)/HP;실험조효원원섬유분포밀도(122±22)/μm2,대조조(218±10)/μm2,2조차이유통계학의의(P＜0.05).결론 극상인대재AS질병진전과정중출현일계렬적조직병이학개변.
Objective To observe the histopathological and ultrastructural changes of the supraspinal ligaments in ankylosing spondylitis (AS).Methods The supraspinal ligaments were stained with HE,Sirius Red/saturated carbazotic acid and then observed nuder microscope,electyonic scanning microscope and transmission electron microscope respectively.The density of blood vessel and collagen fibril were analy-zed.Statistical analysis was carried out by using t test.Results In the AS group,under the microscope,the arrangement of in collagen fibers was in chaos,and hyalinosis,fatty infiltration could be seen.Vascular proliferation was predominant.Collagen-Ⅲ expression could be detected in this group.Under electron microscope,the distribution of collagen fibril was sparse,and the periodic strita was indistinct,the matrix was degraded,and some matrix vesicle could be seen.While in the control group,orderly arrangd collagen fibrils could be seen,and no change of blood vessels could be seen.The density of vascular structures in the AS group was (6.8±0.7)/HP,and (1.0±0.4)/HP of the control group.The density of collagen fibrils in the AS group was (122±22)/μm2,and that of the control group was (218±10)/μm2,the difference between the two groups was statistically significant (P＜0.05).Conclusion A series of histopathological changes have occurred in the supraspinal ligaments in AS patients.