CAJ | 학술논문

目的探讨端粒保护蛋白TPP1及POT1在类风湿关节炎(RA)发病机制中可能的作用.方法体外培养纤维滑膜细胞(SFs)用实时荧光定量聚合酶链反应(PCR)法检测42例RA、23例骨关节炎和13例外伤截肢患者(对照组)SFs TPP1、POT1 mRNA的表达水平,蛋白印迹法检测RA、骨关节炎及健康对照SFs个各8例TPP1、POT1蛋白水平；多组间变量的差异用单因素方差分析,变量间的相关关系采用Spearman相关分析.结果 TPP1、POT1 mRNA在RA组SFs的表达低于骨关节炎组及对照组(TPP1:0.006±0.003,0.179±0.077,0.189±0.027,POT1:0.006±0.003,0.063±0.027,0.072±0.006；P均＜0.01),骨关节炎组和对照组间差异无统计学意义(P＞0.05).TPP1蛋白在RA组的表达显著低于骨关节炎组和对照组(0.225±0.018,0.632±0.030,0.651±0.038;P＜0.01),骨关节炎组和对照组间差异无统计学意义(P＞0.05)；POT1蛋白在3组间差异无统计学意义(P＞0.05).TPP1 mRNA表达量与RA患者抗环瓜氨酸肽抗体及类风湿因子呈负相关(P均＜0.05),与疾病活动评分、红细胞沉降率、血清C反应蛋白无相关性(P均＞0.05)；TPP1、POT1蛋白表达量及POT1 mRNA表达量与上述指标均无相关性(P均＞0.05).结论端粒保护蛋白TPP1、POT1的降低可能在RA患者SFs出现转化细胞特性中发挥重要作用；TPP1转录及蛋白水平的降低可能与RA出现关节侵蚀性改变密切相关.
목적 탐토단립보호단백TPP1급POT1재류풍습관절염(RA)발병궤제중가능적작용.방법 체외배양섬유활막세포(SFs)용실시형광정량취합매련반응(PCR)법검측42례RA、23례골관절염화13예외상절지환자(대조조)SFs TPP1、POT1 mRNA적표체수평,단백인적법검측RA、골관절염급건강대조SFs개각8례TPP1、POT1단백수평；다조간변량적차이용단인소방차분석,변량간적상관관계채용Spearman상관분석.결과 TPP1、POT1 mRNA재RA조SFs적표체저우골관절염조급대조조(TPP1:0.006±0.003,0.179±0.077,0.189±0.027,POT1:0.006±0.003,0.063±0.027,0.072±0.006；P균＜0.01),골관절염조화대조조간차이무통계학의의(P＞0.05).TPP1단백재RA조적표체현저저우골관절염조화대조조(0.225±0.018,0.632±0.030,0.651±0.038;P＜0.01),골관절염조화대조조간차이무통계학의의(P＞0.05)；POT1단백재3조간차이무통계학의의(P＞0.05).TPP1 mRNA표체량여RA환자항배과안산태항체급류풍습인자정부상관(P균＜0.05),여질병활동평분、홍세포침강솔、혈청C반응단백무상관성(P균＞0.05)；TPP1、POT1단백표체량급POT1 mRNA표체량여상술지표균무상관성(P균＞0.05).결론 단립보호단백TPP1、POT1적강저가능재RA환자SFs출현전화세포특성중발휘중요작용；TPP1전록급단백수평적강저가능여RA출현관절침식성개변밀절상관.
Objective To determine whether changes of telomeric proteins TPP1 and POT1 in synovial fibroblasts (SFs) might participate in the pathogenesis of rheumatoid arthritis (RA).Methods SFs were isolated from patients with RA,osteoarthritis (OA) and traumatic joint disease,and cultured in vitro.TPP1 and POT1 mRNA level of SFs was measured using real-time quantitative polymerase chain reaction (RTqPCR) in 42 RA,23 OA and 13 conrtal cases.Western blotting was used to detect the expression of TPP1 and POT1 protein.One-way analysis of variance and Spearman's rank correlation test were performed.Results Expression of TPP1 and POT1 mRNA was significantly reduced in RA cases (TPP1:0.006 ± 0.003,0.179±0.077,0.189±0.027; POT1:0.006±0.003,0.063±0.027,0.072±0.006,P＜0.01; respecti-vely),no significant difference was observed between OA and conrtal cases (P＞0.05,respectively).Western blotting demonstrated that TPP1 protein level was significantly reduced in RA cases (0.225±0.018,0.632±0.030,0.651±0.038; P＜0.01),POT1 protein expression was not statistically significant among RA,OA patients and conrtal subjects (P＞0.05).Significant negative correlation was observed between levelly of TPP1 mRNA and titers of anti-CCP antibody,RF (P＜0.01).Conclusion Changes of TPP1 and POT1 might contribute to persistent proliferation of SFs in RA.