中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2013年
5期
337-340
,共4页
杨洁%刘秀梅%傅自力%郝轶群%罗东萍%王凯
楊潔%劉秀梅%傅自力%郝軼群%囉東萍%王凱
양길%류수매%부자력%학질군%라동평%왕개
红斑狼疮,系统性%蛋白质酪氨酸激酶%脯氨酸
紅斑狼瘡,繫統性%蛋白質酪氨痠激酶%脯氨痠
홍반랑창,계통성%단백질락안산격매%포안산
Lupus erythematosus,systemic%Protein-tyrosine kinase%Proline
目的 探讨富含脯氨酸的酪氨酸激酶2(Pyk2)在系统性红斑狼疮(SLE)发病过程中可能的作用机制.方法 采用反转录-聚合酶链反应(RT-PCR)法检测50例SLE患者和36名健康对照组成员外周血单个核细胞(PBMCs)中Pyk2的表达水平.用Pyk2特异性的抑制剂(TyrA9)抑制Pyk2的活化后,半定量PCR方法检测PBMCs中Blys的表达情况.采用单因素方差分析和Pearson相关分析进行统计学分析.结果 SLE患者Pyk2的表达水平(28.31±0.91)显著高于健康对照组(33.69±0.04),差异有统计学意义(P＜0.05)；促进Pyk2的活化后,SLE患者PBMCs中Blys的表达水平增高；而抑制Pyk2的活化后,Blys的表达水平明显下降.结论 Pyk2可能通过参与淋巴细胞的异常活化而导致SLE的发病.Pyk2的表达与SLE的疾病活动性相关,随着SLE病情的加重,Pyk2的表达水平亦明显增加.此外,合并有肾脏受累的患者Pyk2的表达水平明显高于其他脏器受累者.
目的 探討富含脯氨痠的酪氨痠激酶2(Pyk2)在繫統性紅斑狼瘡(SLE)髮病過程中可能的作用機製.方法 採用反轉錄-聚閤酶鏈反應(RT-PCR)法檢測50例SLE患者和36名健康對照組成員外週血單箇覈細胞(PBMCs)中Pyk2的錶達水平.用Pyk2特異性的抑製劑(TyrA9)抑製Pyk2的活化後,半定量PCR方法檢測PBMCs中Blys的錶達情況.採用單因素方差分析和Pearson相關分析進行統計學分析.結果 SLE患者Pyk2的錶達水平(28.31±0.91)顯著高于健康對照組(33.69±0.04),差異有統計學意義(P＜0.05)；促進Pyk2的活化後,SLE患者PBMCs中Blys的錶達水平增高；而抑製Pyk2的活化後,Blys的錶達水平明顯下降.結論 Pyk2可能通過參與淋巴細胞的異常活化而導緻SLE的髮病.Pyk2的錶達與SLE的疾病活動性相關,隨著SLE病情的加重,Pyk2的錶達水平亦明顯增加.此外,閤併有腎髒受纍的患者Pyk2的錶達水平明顯高于其他髒器受纍者.
목적 탐토부함포안산적락안산격매2(Pyk2)재계통성홍반랑창(SLE)발병과정중가능적작용궤제.방법 채용반전록-취합매련반응(RT-PCR)법검측50례SLE환자화36명건강대조조성원외주혈단개핵세포(PBMCs)중Pyk2적표체수평.용Pyk2특이성적억제제(TyrA9)억제Pyk2적활화후,반정량PCR방법검측PBMCs중Blys적표체정황.채용단인소방차분석화Pearson상관분석진행통계학분석.결과 SLE환자Pyk2적표체수평(28.31±0.91)현저고우건강대조조(33.69±0.04),차이유통계학의의(P＜0.05)；촉진Pyk2적활화후,SLE환자PBMCs중Blys적표체수평증고；이억제Pyk2적활화후,Blys적표체수평명현하강.결론 Pyk2가능통과삼여림파세포적이상활화이도치SLE적발병.Pyk2적표체여SLE적질병활동성상관,수착SLE병정적가중,Pyk2적표체수평역명현증가.차외,합병유신장수루적환자Pyk2적표체수평명현고우기타장기수루자.
Objective To explore the possible role of proline-rich tyrosine kinase (Pyk2) in the pathogenesis of systemic lupus erythematosus (SLE).Methods The expression of Pyk2 in the peripheral blood mononuclear cells (PBMCs) of 50 patients with SLE and 36 healthy controls were tested with RT-PCR assay.The activation of Pyk2 was inhibited using specific inhibitor Pyk2 (TyrA9).Semi-quantitative PCR methodwas used to detect the Blys expression of PBMCs.One-way ANOVA and Pearson's correlation analysis were used for statistical analysis.Results The Pyk2 expression level (28.31 ±0.91) of SLE patients was significantly higher than that in healthy controls (33.69±0.04),the difference was statistically significant (P＜0.05).The activation of Pyk2 was stimulated and the expression levels of Blys in the PBMCs of patients with SLE was elevated.By inhibiting the activation of Pyk2,the BLyS expression levels decreased significantly.Conclusion Pyk2 may be involved in the abnormal activation of lymphocytes which lead to the pathogenesis of SLE.Pyk2 expression is associated with SLE disease activity,disease aggravation,and the Pyk2 expression levels is also increased significantly.In addition,the expression level of Pyk2 is higher in patients with renal involvement than those patients with other organ involvement.
