中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2013年
6期
369-372
,共4页
孟洁%俞大亮%厉小梅%李向培%汪国生
孟潔%俞大亮%厲小梅%李嚮培%汪國生
맹길%유대량%려소매%리향배%왕국생
干燥综合征%白细胞介素-17%B淋巴细胞活化因子
榦燥綜閤徵%白細胞介素-17%B淋巴細胞活化因子
간조종합정%백세포개소-17%B림파세포활화인자
Sj(o)gren's syndrome%Interleukin-17%B cell activating factor of TNF family
目的 检测原发性干燥综合征(pSS)患者血清和唇腺中B淋巴细胞活化因子(BAFF)的表达,通过BAFF、白细胞介素(IL)-17干扰扩增的Th17细胞与B细胞混合培养,探讨它们对B细胞功能的影响.方法 选择确诊的pSS患者40例,酶联免疫吸附试验法检测pSS患者和健康对照血清中BAFF的表达；22例pSS做了唇腺活检和免疫组织化学染色；5例pSS采用流式细胞仪、免疫印迹法检测与BAFF和IL-17干扰扩增的Th17细胞共培养的B细胞凋亡、增殖及抗体产生.采用t检验进行统计学处理.结果 BAFF表达于pSS患者唇腺组织浸润的淋巴细胞及导管上皮细胞,腺泡与对照组几乎无表达.灶性浸润组BAFF阳性细胞数[(888±372)个]高于非灶性浸润组[(164±161)个](t=5.94,P＜0.05)；BAFF阳性的淋巴细胞数占总淋巴细胞的比率[(0.18±0.08)％]高于非灶性浸润组[(0.09±0.07)％](t=3.03,P＜0.05)；血清中pSS患者BAFF水平[(6.0±2.8)ng/ml]显著高于健康对照组[(3.8±1.7)ng/ml] (t=3.26,P＜0.05)；BAFF、IL-17转染组B细胞凋亡率分别为[(24±5)％、(23±5)％]显著高于未转染组[(7±4)％](t值分别为4.6,4.4；P＜0.05),与单独培养的B细胞组相比差异无统计学意义(P＞0.05).与对照组比较,部分培养的上清液中未检测出抗体.结论 BAFF可能参与pSS局部炎症的损伤过程,其与IL-17对B细胞功能异常可能有协同作用.
目的 檢測原髮性榦燥綜閤徵(pSS)患者血清和脣腺中B淋巴細胞活化因子(BAFF)的錶達,通過BAFF、白細胞介素(IL)-17榦擾擴增的Th17細胞與B細胞混閤培養,探討它們對B細胞功能的影響.方法 選擇確診的pSS患者40例,酶聯免疫吸附試驗法檢測pSS患者和健康對照血清中BAFF的錶達；22例pSS做瞭脣腺活檢和免疫組織化學染色；5例pSS採用流式細胞儀、免疫印跡法檢測與BAFF和IL-17榦擾擴增的Th17細胞共培養的B細胞凋亡、增殖及抗體產生.採用t檢驗進行統計學處理.結果 BAFF錶達于pSS患者脣腺組織浸潤的淋巴細胞及導管上皮細胞,腺泡與對照組幾乎無錶達.竈性浸潤組BAFF暘性細胞數[(888±372)箇]高于非竈性浸潤組[(164±161)箇](t=5.94,P＜0.05)；BAFF暘性的淋巴細胞數佔總淋巴細胞的比率[(0.18±0.08)％]高于非竈性浸潤組[(0.09±0.07)％](t=3.03,P＜0.05)；血清中pSS患者BAFF水平[(6.0±2.8)ng/ml]顯著高于健康對照組[(3.8±1.7)ng/ml] (t=3.26,P＜0.05)；BAFF、IL-17轉染組B細胞凋亡率分彆為[(24±5)％、(23±5)％]顯著高于未轉染組[(7±4)％](t值分彆為4.6,4.4；P＜0.05),與單獨培養的B細胞組相比差異無統計學意義(P＞0.05).與對照組比較,部分培養的上清液中未檢測齣抗體.結論 BAFF可能參與pSS跼部炎癥的損傷過程,其與IL-17對B細胞功能異常可能有協同作用.
목적 검측원발성간조종합정(pSS)환자혈청화진선중B림파세포활화인자(BAFF)적표체,통과BAFF、백세포개소(IL)-17간우확증적Th17세포여B세포혼합배양,탐토타문대B세포공능적영향.방법 선택학진적pSS환자40례,매련면역흡부시험법검측pSS환자화건강대조혈청중BAFF적표체；22례pSS주료진선활검화면역조직화학염색；5례pSS채용류식세포의、면역인적법검측여BAFF화IL-17간우확증적Th17세포공배양적B세포조망、증식급항체산생.채용t검험진행통계학처리.결과 BAFF표체우pSS환자진선조직침윤적림파세포급도관상피세포,선포여대조조궤호무표체.조성침윤조BAFF양성세포수[(888±372)개]고우비조성침윤조[(164±161)개](t=5.94,P＜0.05)；BAFF양성적림파세포수점총림파세포적비솔[(0.18±0.08)％]고우비조성침윤조[(0.09±0.07)％](t=3.03,P＜0.05)；혈청중pSS환자BAFF수평[(6.0±2.8)ng/ml]현저고우건강대조조[(3.8±1.7)ng/ml] (t=3.26,P＜0.05)；BAFF、IL-17전염조B세포조망솔분별위[(24±5)％、(23±5)％]현저고우미전염조[(7±4)％](t치분별위4.6,4.4；P＜0.05),여단독배양적B세포조상비차이무통계학의의(P＞0.05).여대조조비교,부분배양적상청액중미검측출항체.결론 BAFF가능삼여pSS국부염증적손상과정,기여IL-17대B세포공능이상가능유협동작용.
Objective To identify interleukin 17 (IL-17) and B cell activating factor (BAFF) that could influence B cell biology by detecting the expression of BAFF in the serum and labial salivary glands from primary Sj(o)gren's syndrome (pSS) patients and to test the apoptosis rates of B cells cultured with Th17 cells which were transfected with IL-17-siRNA,BAFF-siRNA.Methods A total of 40 patients with pSS who were referred to the Department of Rheumatology and Immunology at Anhui Provincial Hospital from June 2011 to June 2012 were enrolled into this study.The expression of BAFF on salivary gland and serum from pSS patients and healthy controls were detected by ELISA and immunohistochemical examination (22 patients with pSS).Flow cytometry was used to detect B cell's apoptosis,BAFF and IL-17 interfered with amplified Th17 cells,and co-cultured with B cells.Immunoblot was used to detect supernatant antibody in 5 patients with pSS.Independent samples t test was used for statistical analysis.Results In all pSS specimens,infiltrating inflammatory cells expressed BAFF,so did some ductual cells,but acinar cells did not express these markers.There was no expression of BAFF in the controls.BAFF-positive cell numbers in the labial salivary glands of pSS patients with focal infiltrating lymphocytes were more than that with non-focal infiltrating lymphocytes (888±372 vs 164±161,t=5.94,P＜0.05),and the percentage of BAFF-positive lymphocytes over the total infiltrating lymphocytes in the salivary glands of pSS patients with focal infiltrating lymphocytes [(0.18 ±0.08) ％] was higher than those with non-focal infiltrating lymphocytes [(0.09 ±0.07) ％] (t =3.03,P＜0.05).The level of soluble BAFF in patients with pSS [(6.0±2.8) ng/ml] was significantly higher than the controls [(3.8±1.7) ng/ml,t=3.26,P＜0.05].BAFF or IL-17 transfected group,B cell apoptosis rate [(24± 5)％,(23±5)％] were significantly higher than the non-transfected group [(7±4)％],t=4.6,4.4; P＜0.05].And there was no significant difference when compared with cultured B cells (P＞0.05).Compared with the controls,no antibody could be detected in the supernatants.Conclusion BAFF may be involved in the process of local inflammatory damage of the pSS,it may have a synergistic effect with IL-17 on abnormal B cell function.
