CAJ | 학술논문

目的探讨抗膜联蛋白A2抗体与类风湿关节炎(RA)患者疾病活动度和骨代谢指标的相关性.方法 ①采用酶联免疫吸附试验(ELISA)法对1 18例RA患者、65例系统性红斑狼疮(SLE)患者、30例其他结缔组织病患者(包括硬皮病、混合性结缔组织病、干燥综合征、抗磷脂综合征等)及35名健康对照者进行抗膜联蛋白A2抗体检测,分析抗膜联蛋白A2抗体在各结缔组织病患者中的阻性率.②详细记录118例RA患者各项临床及实验室指标、腰椎(L1～4)和大转子骨密度值,评价抗膜联蛋白A2抗体与RA临床表现、疾病活动度及骨代谢指标的相关性.采用成组t检验、秩和检验及x2检验进行统计学分析.结果 ①结缔组织病患者中,抗膜联蛋白A2抗体的阳性率分别为:RA 17例(14.4％),SLE 18例(27.7％),其他结缔组织病5例(16.7％);35名健康对照者中,抗膜联蛋白A2抗体的阳性率为0;②在118例RA患者中,抗膜联蛋白A2抗体阳性患者的受累关节数(肿胀和成疼痛的关节数)明显高于抗膜联蛋白A2抗体阴性患者,差异有统计学意义(P＜0.05);③在118例RA患者中,抗膜联蛋白A2抗体阳性患者血清N端骨钙素水平、既往骨折发生率、腰椎(L3、L4)骨密度值明显高于抗膜联蛋白A2抗体阴性患者,差异有统计学意义(P＜0.05).结论抗膜联蛋白A2抗体可在多种自身免疫病中出现.抗膜联蛋白A2抗体可能作为反映骨代谢的有用指标.
목적 탐토항막련단백A2항체여류풍습관절염(RA)환자질병활동도화골대사지표적상관성.방법 ①채용매련면역흡부시험(ELISA)법대1 18례RA환자、65례계통성홍반랑창(SLE)환자、30례기타결체조직병환자(포괄경피병、혼합성결체조직병、간조종합정、항린지종합정등)급35명건강대조자진행항막련단백A2항체검측,분석항막련단백A2항체재각결체조직병환자중적조성솔.②상세기록118례RA환자각항림상급실험실지표、요추(L1～4)화대전자골밀도치,평개항막련단백A2항체여RA림상표현、질병활동도급골대사지표적상관성.채용성조t검험、질화검험급x2검험진행통계학분석.결과 ①결체조직병환자중,항막련단백A2항체적양성솔분별위:RA 17례(14.4％),SLE 18례(27.7％),기타결체조직병5례(16.7％);35명건강대조자중,항막련단백A2항체적양성솔위0;②재118례RA환자중,항막련단백A2항체양성환자적수루관절수(종창화성동통적관절수)명현고우항막련단백A2항체음성환자,차이유통계학의의(P＜0.05);③재118례RA환자중,항막련단백A2항체양성환자혈청N단골개소수평、기왕골절발생솔、요추(L3、L4)골밀도치명현고우항막련단백A2항체음성환자,차이유통계학의의(P＜0.05).결론 항막련단백A2항체가재다충자신면역병중출현.항막련단백A2항체가능작위반영골대사적유용지표.
Objective To evaluate the prevalence of anti-annexin A2 antibodies in patients with rheumatoid arthritis (RA) and their clinical significance.Methods ① Anti-annexin A2 antibodies were detected with enzyme-linked immunosorbent assay (ELISA) in the serum of patients with rheumatoid arthritis (n=118),systemic lupus erythematosus (n=65),other connective tissue diseases (n=30,including systemic sclerosis,MCTD,SS,APS et al).Healthy blood donors (n=35) were used as controls.② The clinical features,laboratory data,bone mineral density (BMD) of lumbar (1-4) and trochanter in 118 patients with RA were recorded in detail.Possible relationship between autoantibody to annexin A2 and clinical features,disease activity and bone metabolism markers in RA were analyzed.Comparisons between groups were performed by t-test,rank-sum test orx2 test.Results ① Anti-annexin A2 antibodies were significantly more prevalent in patients with rheumatoid arthritis (14.4％),systemic lupus erythematosus (27.7％),and other connective tissue diseases (16.7％),than in controls (0).② Compared with those RA patients with negative anti-annexin A2 antibodies,the occurrences of swollen and/or tender joints were significantly higher in the anti-annexin A2 positive patients (P＜0.05).③The serum level of N-OST,the incidence of fracture,and the BMD of lumbar 3-4 were higher in the anti-annexin A2 positive patients (P＜0.05).Conclusion Anti-annexin A2 antibodies can be detected in some autoimmune diseases.Anti-annexin A2 antibodies may be associ-ated with inflammatory activity,and possibly a significant marker of bone metabolism.