CAJ | 학술논문

目的检测pSS患者血浆中维生素D3的水平,探讨其与B细胞亚群相关性.方法采用ELISA法检测55例pSS患者与32名健康对照组血浆维生素D3水平并比较;采用流式细胞术检测17例pSS患者外周血CD19+CD27-初始B细胞、CD19+CD27+记忆性B细胞、CD19+CD27high浆细胞占CD19+B细胞的百分率,并与22名健康对照组比较,维生素D3水平与SS疾病活动指数(SSDAI)、B细胞亚群、泪流率、唾液流率、RF、IgA、IgM、IgG等进行相关分析.统计学方法采用t检验、x2检验、非参数检验、单因素方差分析及Pearman和Spearman相关分析.结果 ①pSS患者1,25-二羟维生素D3[1,25(OH)2D3]显著低于健康对照组[24.17 (22.20,28.41) pg/ml与41.25 (23.38,62.18)pg/ml,P＜0.05]、活动组[22.64(20.74,24.90) pg/ml]低于对照组(P＜0.05],活动组低于稳定组[25.39(23.16,33.09) pg/ml,P＜0.05],稳定组与健康对照组差异无统计学意义(P＞0.05].②pSS患者外周血CD 19+CD27high浆细胞、CD19+CD27+记忆性B细胞占CD19+B细胞的百分率低于健康对照组[(0.89±0.30)％与(1.72±0.43)％;(24±8)％与(34±5)％,P＜0.05],活动组[(1.03±0.59)％;(26±10)％]低于对照组(P＜0.05),稳定组[(1.00±0.16)％;(26±3)％]低于健康对照组(P＜0.05),活动组与稳定组之间差异无统计学意义(P＞0.05); pSS患者CD19+CD27-初始B细胞占CD19+B细胞的百分率高于健康对照组[(75.4±7.5)％与(63.9±5.2)％,P＜0.05],活动组[(73.4±9.7)％]以及稳定组[(73.3±2.9)％]与高于健康对照组(P＜0.05),活动组与稳定组之间差异无统计学意义(P＞0.05).③1,25(OH)2D3水平与pSS组记忆性B细胞百分率、IgG水平呈负相关(r=-0.627,P=0.039; r=0.657,P＜0.001).结论维生素D3表达异常可能在pSS发病机制中起作用.
목적 검측pSS환자혈장중유생소D3적수평,탐토기여B세포아군상관성.방법 채용ELISA법검측55례pSS환자여32명건강대조조혈장유생소D3수평병비교;채용류식세포술검측17례pSS환자외주혈CD19+CD27-초시B세포、CD19+CD27+기억성B세포、CD19+CD27high장세포점CD19+B세포적백분솔,병여22명건강대조조비교,유생소D3수평여SS질병활동지수(SSDAI)、B세포아군、루류솔、타액류솔、RF、IgA、IgM、IgG등진행상관분석.통계학방법채용t검험、x2검험、비삼수검험、단인소방차분석급Pearman화Spearman상관분석.결과 ①pSS환자1,25-이간유생소D3[1,25(OH)2D3]현저저우건강대조조[24.17 (22.20,28.41) pg/ml여41.25 (23.38,62.18)pg/ml,P＜0.05]、활동조[22.64(20.74,24.90) pg/ml]저우대조조(P＜0.05],활동조저우은정조[25.39(23.16,33.09) pg/ml,P＜0.05],은정조여건강대조조차이무통계학의의(P＞0.05].②pSS환자외주혈CD 19+CD27high장세포、CD19+CD27+기억성B세포점CD19+B세포적백분솔저우건강대조조[(0.89±0.30)％여(1.72±0.43)％;(24±8)％여(34±5)％,P＜0.05],활동조[(1.03±0.59)％;(26±10)％]저우대조조(P＜0.05),은정조[(1.00±0.16)％;(26±3)％]저우건강대조조(P＜0.05),활동조여은정조지간차이무통계학의의(P＞0.05); pSS환자CD19+CD27-초시B세포점CD19+B세포적백분솔고우건강대조조[(75.4±7.5)％여(63.9±5.2)％,P＜0.05],활동조[(73.4±9.7)％]이급은정조[(73.3±2.9)％]여고우건강대조조(P＜0.05),활동조여은정조지간차이무통계학의의(P＞0.05).③1,25(OH)2D3수평여pSS조기억성B세포백분솔、IgG수평정부상관(r=-0.627,P=0.039; r=0.657,P＜0.001).결론 유생소D3표체이상가능재pSS발병궤제중기작용.
Objective To investigate the plasma vitamin D3 level and its association with B cell subsets of patients with primary Sj(o)gren's syndrome (pSS).The role of vitamin D3 levels in the pathogenesis of pSS was explored.Methods The expression of plasma vitamin D3 levels of 55 patients with pSS and 32 controls were analyzed by ELISA.Frequencies of peripheral blood CD19+CD27-na(i)ve B cells,CD19+CD27+ memory B cells and CD19+CD27high plasma cells were analyzed by flow cytometry in 34 pSS patients without therapy and 22 controls.The relationship between the vitamin D3 levels and B cell subsets,SSDAI,tear flow rate,saliva flow rate,rheumatoid factor,immunoglobulin was analyzed in pSS patients.Non-parametric test,t test,one-way ANOVA,x2test,Pearman's and Spearman's correlation analysis were used for statistical analysis.Results ① There was significant difference in the levels of plasma 1,25 (OH)2D3 between the pSS patients group and normal control group,1,25 (OH)2D3 was significantly lower in pSS patients than that in the normal control group [24.17(22.20,28.41) pg/ml and 41.25(23.38,62.18) pg/ml,P＜0.05],and that was also obviously lower in the active group [22.64(20.74,24.90) pg/ml] than that in the normal control group (P＜0.05),and that was also obviously lower in the active disease group than that in the inactive disease group [25.39 (23.16,33.09) pg/ml,P＜0.05],but there was no difference between the inactive group and the normal control group (P＞0.05).② The percentage of peripheral blood of CD19+CD27high plasma cells and CD19+CD27+ memory B cells in CD19+ cells was reduced in patients in the pSS group compared with the control group [(0.89±0.30)％ and (1.72±0.43)％,(24±8)％ and (34±5)％; P＜0.05],and that was also significantly lower in the active group [(1.03±0.59) ％ ; (26± 10)％] and inactive group [(1.00±0.16)％,(26± 3)％] than that in the normal controls (P＜0.05).However,there was no difference between the active group and the inactive group (P＞0.05),but the frequency of peripheral blood of CD19+ CD27-naive B cells in CD19+ B cells was increased in patients with pSS compared with normal control group [(75.4±7.5)％ and (63.9±5.2)％,P＜0.05],and that was also significantly higher in the active group [(73.4±9.7)％] and inactive group [(73.3±2.9)％] than that in the normal control (P＜0.05),there was no difference between the active group and the inactive group.③ Significant negative correlation was observed between 1,25 (OH)2D3 and the percentage of peripheral blood CD19+CD27+ memory B cells in CD19+ cells as well as immunoglobulin G(r=-0.627,P=0.039; r=-0.657,P＜0.01) level.Conclusions These results demonstrate that abnormality of vitamin D levels may play an important role in the pathogenesis of pSS.