中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2014年
5期
297-301
,共5页
许荣%赵蓉%钱柳%胡朝英%苗平%余奇文%李云春%何东仪%张冬青
許榮%趙蓉%錢柳%鬍朝英%苗平%餘奇文%李雲春%何東儀%張鼕青
허영%조용%전류%호조영%묘평%여기문%리운춘%하동의%장동청
关节炎,类风湿%抗原呈递%γδ T细胞%效应记忆型Vγ9Vδ2 T细胞
關節炎,類風濕%抗原呈遞%γδ T細胞%效應記憶型Vγ9Vδ2 T細胞
관절염,류풍습%항원정체%γδ T세포%효응기억형Vγ9Vδ2 T세포
Arthritis,rheumatoid%Antigen presentation%γδ T cells%Effector memory Vγ9Vδ2 T cells
目的 分析RA患者外周血和关节滑膜液中γδ T细胞亚群及其与RA发病的关联性.方法 采用流式细胞仪检测健康人外周血、RA患者外周血PBMCs及滑膜液单个核细胞(SFMCs)中Vγ9Vδ2T细胞亚群;ELISA法检测在OA患者滑膜液、RA患者血清和滑膜液、RA患者PBMCs和SFMCs培养上清中IFN-γ和IL-17的表达水平;氚-胸腺嘧啶核苷(3H-TdR)掺入法检测PBMCs和SFMCs对抗原肽的应答能力,以及γδ T细胞的抗原递呈能力.采用t检验、单因素方差分析进行统计学分析.结果 RA患者外周血中效应记忆型Vγ9Vδ2 T细胞占γδ T细胞的(48.2±17.6)％,并有(42.8±17.7)％和(2.2±1.5)％的Vγ9Vδ2 T细胞表面分别表达HLA-DR4和CD86分子;关节滑液中效应记忆型Vγ9Vδ2 T细胞占γδ T细胞的(62.9±7.9)％,并有(85.8±1.7)％和(17.3±2.0)％的Vγ9Vδ2 T细胞表面分别表达HLA-DR4和CD86分子.RA患者的PBMCs和SFMCs具有分泌IFN-γ和IL-17炎症因子的特征,且较健康人PBMCs相比,对抗原肽具有更强的免疫反应.同时实验结果还表明RA患者的γδ T细胞的抗原递呈能力较B细胞强.结论 γδ T细胞在RA的发病过程中不仅通过分泌细胞因子与炎症细胞协同作用参与炎症反应,而且通过特异性免疫应答的机制发挥树突状细胞样的作用,不断将病毒肽和自身抗原肽递呈给CD4+T细胞.
目的 分析RA患者外週血和關節滑膜液中γδ T細胞亞群及其與RA髮病的關聯性.方法 採用流式細胞儀檢測健康人外週血、RA患者外週血PBMCs及滑膜液單箇覈細胞(SFMCs)中Vγ9Vδ2T細胞亞群;ELISA法檢測在OA患者滑膜液、RA患者血清和滑膜液、RA患者PBMCs和SFMCs培養上清中IFN-γ和IL-17的錶達水平;氚-胸腺嘧啶覈苷(3H-TdR)摻入法檢測PBMCs和SFMCs對抗原肽的應答能力,以及γδ T細胞的抗原遞呈能力.採用t檢驗、單因素方差分析進行統計學分析.結果 RA患者外週血中效應記憶型Vγ9Vδ2 T細胞佔γδ T細胞的(48.2±17.6)％,併有(42.8±17.7)％和(2.2±1.5)％的Vγ9Vδ2 T細胞錶麵分彆錶達HLA-DR4和CD86分子;關節滑液中效應記憶型Vγ9Vδ2 T細胞佔γδ T細胞的(62.9±7.9)％,併有(85.8±1.7)％和(17.3±2.0)％的Vγ9Vδ2 T細胞錶麵分彆錶達HLA-DR4和CD86分子.RA患者的PBMCs和SFMCs具有分泌IFN-γ和IL-17炎癥因子的特徵,且較健康人PBMCs相比,對抗原肽具有更彊的免疫反應.同時實驗結果還錶明RA患者的γδ T細胞的抗原遞呈能力較B細胞彊.結論 γδ T細胞在RA的髮病過程中不僅通過分泌細胞因子與炎癥細胞協同作用參與炎癥反應,而且通過特異性免疫應答的機製髮揮樹突狀細胞樣的作用,不斷將病毒肽和自身抗原肽遞呈給CD4+T細胞.
목적 분석RA환자외주혈화관절활막액중γδ T세포아군급기여RA발병적관련성.방법 채용류식세포의검측건강인외주혈、RA환자외주혈PBMCs급활막액단개핵세포(SFMCs)중Vγ9Vδ2T세포아군;ELISA법검측재OA환자활막액、RA환자혈청화활막액、RA환자PBMCs화SFMCs배양상청중IFN-γ화IL-17적표체수평;천-흉선밀정핵감(3H-TdR)참입법검측PBMCs화SFMCs대항원태적응답능력,이급γδ T세포적항원체정능력.채용t검험、단인소방차분석진행통계학분석.결과 RA환자외주혈중효응기억형Vγ9Vδ2 T세포점γδ T세포적(48.2±17.6)％,병유(42.8±17.7)％화(2.2±1.5)％적Vγ9Vδ2 T세포표면분별표체HLA-DR4화CD86분자;관절활액중효응기억형Vγ9Vδ2 T세포점γδ T세포적(62.9±7.9)％,병유(85.8±1.7)％화(17.3±2.0)％적Vγ9Vδ2 T세포표면분별표체HLA-DR4화CD86분자.RA환자적PBMCs화SFMCs구유분비IFN-γ화IL-17염증인자적특정,차교건강인PBMCs상비,대항원태구유경강적면역반응.동시실험결과환표명RA환자적γδ T세포적항원체정능력교B세포강.결론 γδ T세포재RA적발병과정중불부통과분비세포인자여염증세포협동작용삼여염증반응,이차통과특이성면역응답적궤제발휘수돌상세포양적작용,불단장병독태화자신항원태체정급CD4+T세포.
Objective To analyze subsets of γδ T cells in rheumatoid arthritis (RA) patients' peripheral blood and synovial fluid and its correlation with the pathogenesis of RA.Methods Flow cytometry was used to analyze the Vγ9Vδ2 T cell subsets in human peripheral blood,peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) of RA patients.ELISA was used to detect IFN-γ and IL-17 in the synovial fluid of osteoarthritis (OA) patients',the serum of RA patients,synovial fluid and the cell culture supernatant of RA patients' PBMCs and SFMCs; 3H-TdR was labeled to detect the proliferation of PBMCs and SFMCs induced by antigen peptides,and the antigen-presenting of γδ T cell.Data were analyzed using t-test and One-way ANOVA analysis.Results The research showed that in γδ T cells of RA patients' peripheral blood,(48.2±17.6)％ were effector memory Vγ9Vδ2 T cells,(42.8±17.7)％ and (2.2±1.5)％ Vγ9Vδ2 T cell expressed HLA-DR4 and CD86.While in RA patients' synovial fluid,(62.9±7.9)％ were effector memory-type Vγ9Vδ2 T cells,(85.8±1.7)％ and (17.3±2.0)％ Vγ9Vδ2 T cell expressed HLA-DR4 and CD86.The PBMCs and SFMCs of RA patients could secrete IFN-γand IL-17,and their antigen peptide immunogenicity was stronger than that of healthy human PBMCs.The results also showed that the antigenpresenting ability of γδ T cells was stronger than B cells.Conclusion The results suggest that γδ T cells participat in the pathogenesis of RA,not only by interacting with inflammatory cells to aggravate autoimmune disorder,but also contributing to specific immune response by acting as DCs-like cells-presenting antigen to CD4+ T cells persistently.
