中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2014年
5期
302-307
,共6页
朱华群%孙晓麟%栗占国%苏茵
硃華群%孫曉麟%慄佔國%囌茵
주화군%손효린%률점국%소인
红斑狼疮,系统性%蛋白S%生长阻抑特异性蛋白6%临床意义
紅斑狼瘡,繫統性%蛋白S%生長阻抑特異性蛋白6%臨床意義
홍반랑창,계통성%단백S%생장조억특이성단백6%림상의의
Lupus erythematosus,systemic%Protein S%Growth-arrest specific protein 6%Clinical significance
目的 探讨蛋白S和生长阻抑特异性蛋白6(Gas6)在SLE中的临床意义.方法 应用ELISA检测103例SLE患者与40名健康对照组血浆中蛋白S和Gas6的浓度,并分析比较其与SLE患者各种临床表现及实验室指标的关系.采用Mann-Whitney U检验、x2检验和Spearman相关性分析进行统计学分析.结果 SLE患者血浆中,蛋白S和Gas6的浓度均明显低于健康对照组[蛋白S:30.6(27.2,33.6)与37.8 (35.4,46.7) μg/ml,Z =6.04,P ＜0.01;Gas6:402.6 (239.2,757.8)与913.6 (765.0,1290.6) pg/ml,Z=4.26,P＜0.01],蛋白S与Gas6浓度呈正相关(r=0.312,P=0.001).血浆蛋白S浓度与SLE患者外周血血红蛋白、白细胞计数、血小板计数、补体C3及补体C4水平呈正相关(r值分别为:0.209,0.264,0.264,0.362,0.280,P＜0.01或P＜0.05),与抗dsDNA抗体、抗心磷脂抗体、抗核小体抗体以及IgG呈负相关(r值分别为-0.197,-0.264,-0.226,-0.229,P＜0.01或P＜0.05);血浆Gas6水平与SLE患者年龄、病程以及CRP呈正相关(r值分别为:0.229,0.198,0.263,P＜0.01或P＜0.05);Gas6减低患者发热、皮疹及浆膜炎发生率显著高于Gas6正常者.SLE患者中,外周血白细胞减低者、蛋白尿阳性者Gas6的浓度低于白细胞、蛋白尿正常者(P＜0.05).结论 SLE患者血浆中蛋白S水平显著降低,并与血液系统损害、补体系统侵及以及自身抗体产生相关;血浆Gas6水平也显著降低,且与年龄、病程、皮肤、肾损害及炎症等临床特点相关.
目的 探討蛋白S和生長阻抑特異性蛋白6(Gas6)在SLE中的臨床意義.方法 應用ELISA檢測103例SLE患者與40名健康對照組血漿中蛋白S和Gas6的濃度,併分析比較其與SLE患者各種臨床錶現及實驗室指標的關繫.採用Mann-Whitney U檢驗、x2檢驗和Spearman相關性分析進行統計學分析.結果 SLE患者血漿中,蛋白S和Gas6的濃度均明顯低于健康對照組[蛋白S:30.6(27.2,33.6)與37.8 (35.4,46.7) μg/ml,Z =6.04,P ＜0.01;Gas6:402.6 (239.2,757.8)與913.6 (765.0,1290.6) pg/ml,Z=4.26,P＜0.01],蛋白S與Gas6濃度呈正相關(r=0.312,P=0.001).血漿蛋白S濃度與SLE患者外週血血紅蛋白、白細胞計數、血小闆計數、補體C3及補體C4水平呈正相關(r值分彆為:0.209,0.264,0.264,0.362,0.280,P＜0.01或P＜0.05),與抗dsDNA抗體、抗心燐脂抗體、抗覈小體抗體以及IgG呈負相關(r值分彆為-0.197,-0.264,-0.226,-0.229,P＜0.01或P＜0.05);血漿Gas6水平與SLE患者年齡、病程以及CRP呈正相關(r值分彆為:0.229,0.198,0.263,P＜0.01或P＜0.05);Gas6減低患者髮熱、皮疹及漿膜炎髮生率顯著高于Gas6正常者.SLE患者中,外週血白細胞減低者、蛋白尿暘性者Gas6的濃度低于白細胞、蛋白尿正常者(P＜0.05).結論 SLE患者血漿中蛋白S水平顯著降低,併與血液繫統損害、補體繫統侵及以及自身抗體產生相關;血漿Gas6水平也顯著降低,且與年齡、病程、皮膚、腎損害及炎癥等臨床特點相關.
목적 탐토단백S화생장조억특이성단백6(Gas6)재SLE중적림상의의.방법 응용ELISA검측103례SLE환자여40명건강대조조혈장중단백S화Gas6적농도,병분석비교기여SLE환자각충림상표현급실험실지표적관계.채용Mann-Whitney U검험、x2검험화Spearman상관성분석진행통계학분석.결과 SLE환자혈장중,단백S화Gas6적농도균명현저우건강대조조[단백S:30.6(27.2,33.6)여37.8 (35.4,46.7) μg/ml,Z =6.04,P ＜0.01;Gas6:402.6 (239.2,757.8)여913.6 (765.0,1290.6) pg/ml,Z=4.26,P＜0.01],단백S여Gas6농도정정상관(r=0.312,P=0.001).혈장단백S농도여SLE환자외주혈혈홍단백、백세포계수、혈소판계수、보체C3급보체C4수평정정상관(r치분별위:0.209,0.264,0.264,0.362,0.280,P＜0.01혹P＜0.05),여항dsDNA항체、항심린지항체、항핵소체항체이급IgG정부상관(r치분별위-0.197,-0.264,-0.226,-0.229,P＜0.01혹P＜0.05);혈장Gas6수평여SLE환자년령、병정이급CRP정정상관(r치분별위:0.229,0.198,0.263,P＜0.01혹P＜0.05);Gas6감저환자발열、피진급장막염발생솔현저고우Gas6정상자.SLE환자중,외주혈백세포감저자、단백뇨양성자Gas6적농도저우백세포、단백뇨정상자(P＜0.05).결론 SLE환자혈장중단백S수평현저강저,병여혈액계통손해、보체계통침급이급자신항체산생상관;혈장Gas6수평야현저강저,차여년령、병정、피부、신손해급염증등림상특점상관.
Objective To investigate the significance of plasma protein S and growth-arrest specific protein 6 (Gas6) in systemic lupus erythematosus (SLE).Methods Sandwich enzyme-linked immunosorbent assay (ELISA) was used to measure the protein S and Gas6 in the plasma of 103 SLE patients and 40 healthy controls.Mann-Whitney U-test,x2-test and Spearman's rank correlation were used for statistical analysis.Results The plasma concentrations of protein S and Gas6 were both significantly [protein S:30.6 (27.2,33.6) μg/ml,37.8(35.4,46.7) μg/ml,Z=6.04,P＜0.01; Gas6:402.6(239.2,757.8) pg/ml,913.6(765.0,1 290.6) pg/ml,Z=4.26,P＜0.01] decreased in plasma of SLE patients than that in healthy controls.There was a positive correlation between levels of protein S and Gas6 (r=0.312,P=0.001).The level of protein S in SLE patients was positively correlated with hemoglobin (Hb),WBC,platelet blood platelet (PLT),C3 and C4 (r=0.209,0.264,0.264,0.362,0.280,P＜0.01 or P＜0.05).Plasma protein S level was also found to be negatively correlated with auto-antibodies such as anti-double strand DNA (anti-dsDNA) antibody,anticardiolipin (ACL),anti-nucleosome antibody (AnuA) or IgG (r=-0.197,-0.264,-0.226,-0.229,P＜0.01or P＜0.05).The plasma Gas6 level was positively associated with age,disease duration and C-reaction protein (CRP) (r=0.229,0.198,0.263,P＜0.01 or P＜0.05).Patients with decreased Gas6 level showed higher incidence of fever,rash and serositis.Patients with decreased WBC or positive for proteinuria also showed decreased Gas6 (P＜0.05).Conclusion Protein S levels are significantly decreased in plasma of SLE patients and is associated with a series of severe disease manifestations such as hematological involvement,decreased complements and the presence of auto-antibodies.Decreased Gas6 levels in SLE patients are observed and they are correlated with age,disease duration and certain clinical characteristics such as rash,renal involvement and inflammatory response.
