CAJ | 학술논문

目的探讨RA患者PBMCs上HLA-G的表达及其与RA疾病活动度、病程、临床用药及各实验室指标的相关关系.方法采用流式细胞术检测68例RA患者和63名健康对照者PBMCs中淋巴细胞、CD3+T细胞和CD14+单核细胞上HLA-G的表达.采用非参数Mann-Whitney U检验、Spearman等级相关分析.结果 RA患者外周血HLA-G+淋巴细胞百分比显著低于健康对照组[1.98％(1.17％～3.66％与2.93％(1.70％～5.40％),U=1624.5,P＜0.05],而且HLA-G+淋巴细胞百分比与RA疾病活动度DAS28评分呈负相关(r=-0.288,P=0.017);CD3+HLA-G+T细胞百分比亦明显降低[1.35％(0.57％～2.79％)与2.16％(1.15％～4.45％),U=1387,P=0.000],且与DAS28评分呈负相关(r=-0.373,P=0.001);CD14+HLA-G+单核细胞百分比明显低于健康对照组[22.65％(10.59％～36.81％)与32.76％(20.73％～44.62％),U=1 526,P＜0.01],但未发现CD14+HLA-G+单核细胞百分比与DAS28评分存在显著相关性(P＞0.05).此外,上述各类细胞上HLA-G的表达与RA患者病程、临床用药及各实验室指标如RF、ESR、CRP、抗CCP抗体和ANA水平等均无显著相关性(均P＞0.05).结论 RA患者外周血淋巴细胞、CD3+T细胞和CD14+单核细胞上HLA-G的表达异常降低,HLA-G分子的异常降低可能导致机体免疫抑制功能低下,从而促进了RA的发生发展.
목적 탐토RA환자PBMCs상HLA-G적표체급기여RA질병활동도、병정、림상용약급각실험실지표적상관관계.방법 채용류식세포술검측68례RA환자화63명건강대조자PBMCs중림파세포、CD3+T세포화CD14+단핵세포상HLA-G적표체.채용비삼수Mann-Whitney U검험、Spearman등급상관분석.결과 RA환자외주혈HLA-G+림파세포백분비현저저우건강대조조[1.98％(1.17％～3.66％여2.93％(1.70％～5.40％),U=1624.5,P＜0.05],이차HLA-G+림파세포백분비여RA질병활동도DAS28평분정부상관(r=-0.288,P=0.017);CD3+HLA-G+T세포백분비역명현강저[1.35％(0.57％～2.79％)여2.16％(1.15％～4.45％),U=1387,P=0.000],차여DAS28평분정부상관(r=-0.373,P=0.001);CD14+HLA-G+단핵세포백분비명현저우건강대조조[22.65％(10.59％～36.81％)여32.76％(20.73％～44.62％),U=1 526,P＜0.01],단미발현CD14+HLA-G+단핵세포백분비여DAS28평분존재현저상관성(P＞0.05).차외,상술각류세포상HLA-G적표체여RA환자병정、림상용약급각실험실지표여RF、ESR、CRP、항CCP항체화ANA수평등균무현저상관성(균P＞0.05).결론 RA환자외주혈림파세포、CD3+T세포화CD14+단핵세포상HLA-G적표체이상강저,HLA-G분자적이상강저가능도치궤체면역억제공능저하,종이촉진료RA적발생발전.
Objective To investigate the expression of HLA-G in lymphocytes,CD3+ T cells,and CD14+ monocytes from patients with rheumatoid arthritis (RA) and their associations with disease activity,disease duration,medication used and clinical parameters.Methods We studied 68 RA patients as well as 63 healthy controls.HLA-G expression was analyzed by flow cytometry in peripheral blood mononuclear cells (PBMCs).Mann Whitney U tests and Spearman rank correlation coefficient test were used for statistical analysis.Results We found that lymphocytes as well as CD3+ T cells,and CD14+ monocytes in RA patients showed a diminished expression of HLA-G compared with healthy controls [1.98％(1.17％-3.66％) vs 2.93％ (1.70％-5.40％),U=1624.5,P＜0.05; 1.35％(0.57％-2.79％) vs 2.16％(1.15％-4.45％),U=1387,P=0.000; 22.65％ (10.59％-36.81％) vs 32.76％ (20.73％-44.62％),U=1526,P＜0.01].Spearman analysis showed that the levels of HLA-G+ lymphocytes as well as CD3+ HLA-G+ T cells was negatively associated with DAS28 score(r=-0.288,P=0.017; r=-0.373,P=0.001),but no significant correlation was detected between the levels of CD14+HLA-G+monocytes and DAS 28 score (P＞0.05).In addition,the levels of expression of HLA-G was not correlated to disease duration,medication used,ESR,CRP,RF,anti-CCP and ANA (P＞0.05).Conclusion Decreased expression of HLA-G are detected in lymphocytes,CD3+ T cells,and CD14+ monocytes from pa-tients with RA,and the abnormality of HLA-G may play an important role in the development of RA.