CAJ | 학술논문

目的探讨超大剂量γ射线离体照射后人外周血淋巴细胞染色体畸变的量效关系,建立双+环(dic+r)大剂量-效应曲线.方法外周静脉血样采自3名健康男性,经60Co γ线(0～50 Gy,剂量率2.35 Gy/min)照射.采用即刻加秋水仙素的微量全血法,分别培养52、72及96 h.计数有丝分裂指数(MI)、双着丝粒体(dic)和环(r)畸变数,拟合dic+r剂量-效应曲线,对2例受大剂量照射的事故患者进行剂量估算.结果 MI随照射剂量的增加而逐渐减少.每细胞dic+r频率随照射剂量增加而增加直到23 Gy(5 Gy之后增加幅度较前变小),＞23 Gy后趋于饱和.对所获数据进行回归分析,拟合dic+r大剂量.效应曲线(5～23 Gy):每细胞dic+r频率γ=-1.608(±0.300)+0.830(±0.051)D-0.013(±0.002)D2(R2=0.998).用拟合曲线对2例受照患者剂量的估箅结果与用物理方法和电子自旋共振(ESR)法估箅的剂量及临床表现基本一致.结论本研究建立的dic+r大剂量-效应曲线,可估计的上限剂量达23 Gy,有可能提高常规染色体畸变分析用作生物剂量计的实用价值.
목적 탐토초대제량γ사선리체조사후인외주혈림파세포염색체기변적량효관계,건립쌍+배(dic+r)대제량-효응곡선.방법 외주정맥혈양채자3명건강남성,경60Co γ선(0～50 Gy,제량솔2.35 Gy/min)조사.채용즉각가추수선소적미량전혈법,분별배양52、72급96 h.계수유사분렬지수(MI)、쌍착사립체(dic)화배(r)기변수,의합dic+r제량-효응곡선,대2례수대제량조사적사고환자진행제량고산.결과 MI수조사제량적증가이축점감소.매세포dic+r빈솔수조사제량증가이증가직도23 Gy(5 Gy지후증가폭도교전변소),＞23 Gy후추우포화.대소획수거진행회귀분석,의합dic+r대제량.효응곡선(5～23 Gy):매세포dic+r빈솔γ=-1.608(±0.300)+0.830(±0.051)D-0.013(±0.002)D2(R2=0.998).용의합곡선대2례수조환자제량적고폐결과 여용물리방법 화전자자선공진(ESR)법고폐적제량급림상표현기본일치.결론 본연구건립적dic+r대제량-효응곡선,가고계적상한제량체23 Gy,유가능제고상규염색체기변분석용작생물제량계적실용개치.
Objective To explore the dose-effect of chromosome aberration,and establish a dose-effect curve of dicentrie and ring (die+r) in human peripheral blood lymphocytes irradiated with high dose of γ-rays.Methods Human peripheral blood was obtained from three healthy individuals,and exposed to 60Co γ-rays with doses between 0 and 50 Gy.Colchicine was added immediately and the cells were incubated at 37℃ for 52,72 and 96 h,respectively.Metaphase index (MI) and dic+r were counted,and a dose-effect curve was fitted.Dose estimation were performed according to the curve for the two patients who had accidentally received high doses of irradiation.Results MI was reduced with the dose increasing.Frequencies of dic+r were increased until 23 Gy,and then saturation was observed.A dose-effect curve was fitted during 5-23 Gy,and the doseeffect was as follows:γ=-1.608(±0.300)+0.830(±0.051) D-0.013(±0.002) D2(R2=0.998),where γ is the yield of dic+r,and D is the dose.The dose estimated for the two patients were in accordance with those by physical method,ESR method and clinical symptoms.Conclusions The highest dose that could be estimated is 23 Gy with the established dic+r dose-effect curve by using the eolchicine black method and prolonged culture time,which suggests the potential of chromosome aberration analysis as biological dosemeter.