CAJ | 학술논문

目的研究低剂量照射(6 cGy)的骨髓细胞悬液,离心后得到的上层相(简称条件液)对正常或辐射损伤细胞能否产生刺激性辐射旁效应,并探讨其发生机制.方法条件液与受0、2或5 Gy照射的骨髓细胞悬液进行混合培养,使用MTT比色法观察各组细胞的增殖能力.同时采用细胞色素C还原法测定培养介质中O2-的浓度,以及运用免疫组织化学法检测细胞内c-fos的蛋白表达.结果受大剂量照射的细胞与条件液共培养后,其增殖能力与对照组比较,差异有统计学意义(P＜0.01),且伴随着O2-浓度升高和c-fos蛋白表达的上调(P＜0.05).结论低剂量辐射可对辐射损伤细胞产生促进其增殖的刺激性辐射旁效应,发生机制可能O2-浓度的提高与c-fos蛋白表达的上调有关.
목적 연구저제량조사(6 cGy)적골수세포현액,리심후득도적상층상(간칭조건액)대정상혹복사손상세포능부산생자격성복사방효응,병탐토기발생궤제.방법 조건액여수0、2혹5 Gy조사적골수세포현액진행혼합배양,사용MTT비색법관찰각조세포적증식능력.동시채용세포색소C환원법측정배양개질중O2-적농도,이급운용면역조직화학법검측세포내c-fos적단백표체.결과 수대제량조사적세포여조건액공배양후,기증식능력여대조조비교,차이유통계학의의(P＜0.01),차반수착O2-농도승고화c-fos단백표체적상조(P＜0.05).결론 저제량복사가대복사손상세포산생촉진기증식적자격성복사방효응,발생궤제가능O2-농도적제고여c-fos단백표체적상조유관.
Objective To investigate whether the supernatant (the conditioned fluid) of myeloid cells suspension after low dose radiation (6 cGy) in vitro could resuh in bormesis on the normal or radiation damage cells and its mechanism.Methods Mice myeloid cell suspension was irradiated by 0,2 and 5 Gy,respectively,and cultured in vitro.MTT method was used to measure the reproductive activity of cells.Cytochrome C reduction method was used to determine the concentration of O2-,the immunohistochemical method to test the protein expression of c-fos.Results Co-cultured with the conditioned fluid,the reproductive activity of the myeloid cells after high dose irradiation( P＜0.01 ),while both the concentration of O2- and the protein expression of c-fos were enhanced (P＜0.05 ).Conclusions The conditioned fluid could enhance the proliferation of the myeloid cells after radiation injury.The mechanism of the low dose radiation-induced bystander effect might be correlated with the increase of both the concentration of O2- and the protein expression of c-fos.