CAJ | 학술논문

目的研究食管鳞癌细胞HIF-1α和COX-2的表达和肿瘤微血管密度(MVD)与食管癌放疗疗效的关系.方法应用免疫组织化学二步法对55例食管鳞癌食管镜活检组织中HIF-1α、COX-2的表达进行检测,并用同样方法检测癌组织中CD34的表达评估MVD.分析其与临床病理学参数及放疗疗效的关系.结果 HIF-1α和COX-2的阳性表达率分别为92.7%和69.1%,MVD为2～29条/高倍视野,平均为(11.2±4.1)条.HIF-1α和COX-2的表达水平与MVD数量无关.HIF-1α和COX-2的表达水平与肿瘤的细胞分化程度、肿瘤部位均无关.单因素分析示食管癌放疗后近期疗效中位生存期和2年生存率与HIF-1α、COX-2表达及MVD无关.结论食管鳞癌组织中HIF-1α和COX-2的表达情况和肿瘤组织中微血管密度无关.HIF-1α和COX-2的表达水平及MVD均不能作为食管鳞癌放射治疗的预后指标.
목적 연구식관린암세포HIF-1α화COX-2적표체화종류미혈관밀도(MVD)여식관암방료료효적관계.방법 응용면역조직화학이보법대55례식관린암식관경활검조직중HIF-1α、COX-2적표체진행검측,병용동양방법 검측암조직중CD34적표체평고MVD.분석기여림상병이학삼수급방료료효적관계.결과 HIF-1α화COX-2적양성표체솔분별위92.7%화69.1%,MVD위2～29조/고배시야,평균위(11.2±4.1)조.HIF-1α화COX-2적표체수평여MVD수량무관.HIF-1α화COX-2적표체수평여종류적세포분화정도、종류부위균무관.단인소분석시식관암방료후근기료효중위생존기화2년생존솔여HIF-1α、COX-2표체급MVD무관.결론 식관린암조직중HIF-1α화COX-2적표체정황화종류조직중미혈관밀도무관.HIF-1α화COX-2적표체수평급MVD균불능작위식관린암방사치료적예후지표.
Objective To investigate the expressions of HIF-1α,COX-2 and introtumoral microvascalar density(MVD) in esophageal aquamous cell carcinoma (ESCC),and their impacts on the efficacy of radiotherapy.Methods Expressions of HIF-1α and COX-2 were assessed by immunohistochemistry methods in fifty-five cases of ESCC.Microvessels were labelled in sections of same tissues by CD34 immunohistochemistry.The correlations with clinicopathological features and postradiotherapy survival rates were evaluated and analyzed.Results The MVD ranged from 2 to 29 ( mean of 11.2±4.1 ).The positive rates of HIF-1α and COX-2 protein expression were 92.7% and 69.1%,respectively,both of which were not correlated with intratumoral MVD.The expressions of HIF-1α and COX-2 were not correlated with histological grade or the location of the primary tumor.Univariate analysis revealed that 2-year survival rates and median survival time following radiotherapy were not assocaited with the expressions of HIF-1α,COX-2 or MVD.Conclusions MVD and the expressions of HIF-1α and COX-2 in ESCC are not efficient to predict the outcome of ESCC following radiotherapy.